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SCREEN-MASTER PLUS Automatic analyser
USER'S GUIDE 610031_4.doc
Page 2 of 36
TABLE OF CONTENTS INTRODUCTION........................................................................................................................................................ 3 HOW TO USE THE MANUAL ................................................................................................................................... 4 1. INSTRUMENT INSTALLATION........................................................................................................................ 5 1.1 Unpacking the instrument.............................................................................................................................. 5 1.2 Instrument description................................................................................................................................... 5 1.3 First installation of the instrument................................................................................................................. 7 1.4 Language selection and printer setting .......................................................................................................... 8 1.5 Instrument maintenance ................................................................................................................................ 9 2 PRINTING......................................................................................................................................................... 10 2.1 Print and display of the analyses name list .................................................................................................. 10 2.2 Print of analytic parameters of one analysis ................................................................................................ 10 2.3 Print of results ............................................................................................................................................ 10 2.4 Print of all analyses parameters .................................................................................................................. 11 3 EXECUTION OF ANALYSES ................................................................................................................................ 12 3.1 Absorbance readings................................................................................................................................... 12 3.2 Selection of the analysis .............................................................................................................................. 12 3.3 Performance of the End-point type analysis with K...................................................................................... 13 3.4 Performance of the whole blood analysis with Haematocrit correction ........................................................ 13 3.5 Performance of the End-point type analysis with standard ........................................................................... 14 3.6 Performance of the Glycohaemoglobin test.................................................................................................. 15 3.7 Performance of the Multistandard type analysis .......................................................................................... 16 3.8 Performance of the Kinetic type analysis ..................................................................................................... 16 3.9 Performance of the Fixed-time type analysis with K..................................................................................... 17 3.10 Performance of the Fixed-time type analysis with standard.......................................................................... 17 3.11 Read with incubator temperature lower then 37 °C. ..................................................................................... 18 3.12 Modification of the progressive number....................................................................................................... 18 3.13 Waste processing ........................................................................................................................................ 19 4 MODIFICATION OF THE ANALYSES PARAMETERS (INCLUDING K AND STANDARD)................................. 20 4.1 Modification of the parameters of an End-point analysis with K................................................................... 22 4.2 Modification of the parameters of an End-point analysis with standard........................................................ 23 4.3 Modification of the parameters of a kinetic analysis..................................................................................... 25 4.4 Modification of the parameters of a Fixed-time analysis with K ................................................................... 27 4.5 Modification of the parameters of a Fixed-time analysis with standard ........................................................ 28 5 CALCULATION PROCEDURES TAKEN BY SCREEN-MASTER PLUS .......................................................... 31 6 TECHNICAL FEATURES................................................................................................................................. 32 7 MESSAGES AND ERROR SIGNALS ............................................................................................................... 33 8 APPENDIX........................................................................................................................................................ 34
EC STATEMENT OF COMPLIANCE
Screen-Master plus - User's guide
Biochemical Systems International s.r.l., via Galileo Ferraris, 220- Arezzo ITALY
Page 3 of 36
INTRODUCTION The Screen-Master plus is an interferential filter analyser, completely managed by microprocessors, which can take photometric measures and process them according to programs with parameters that can be entered by the operator. This instrument has been designed to perform spectroscopic measurements at predetermined wavelengths of analyte concentration and enzyme activity using various reagents. Particularly, the following determinations can be carried out: ABSORBANCE END-POINT (Concentration) KINETICS FIXED-TIME MULTISTANDARD The selection of the interferential filter is automatic, with powered handling managed by a microprocessor. 5 filters are included in the instrument, but it is possible to add a sixth one. This characteristic makes reading easier and eliminates filter selection errors that may occur in the instruments with manual selection. The instrument optics is very sophisticated: there is a high-power halogen lamp (5 W) and the light beam is centered by a quartz lens allowing a high measuring accuracy even when reduced volume cuvettes are used. A 10-place dry incubator is also installed in the instrument for square and round cuvettes. The temperature of the thermostatic unit is equal to the cuvette one, i.e. 37°C. The instrument is programmed by means of a keyboard for the entry of the parameters that are requested by a 32-character alphanumeric display. This display visualizes also the instrument states and error or malfunction signallings. The analyses results are directly displayed in the measuring units pre-selected when programming. The instructions on the display can be chosen in any of the following languages: English, Italian, German and French. The instrument is provided with a 20-column thermal printer that can print both the analyses results and their programming parameters. All printed information is sent also to a serial RS-232 standard output. The use of the instrument is assisted by an advanced software that guides and controls the operations made by the user. The instrument is equipped with an acoustic signal that further helps the user. A sound with a tone different from the usual one warns when you push the wrong key. Sixty programs can be stored. To make an analysis it is necessary to enter all its parameters correctly, including the K and standard values. The instrument is supplied already programmed. Anyhow, it is necessary to check for the pre-entered parameter values correspond to the required ones for the desired methods. A modification of the analytical parameters can be done by the operator before the analysis. The instrument is entirely reprogrammable: however the following programs WBCHOL, HCT, ERY, WBTRIG AND GLYC, reserved for whole blood Cholesterol, Haematocrit, Erythrocytes, whole blood Triglycerides and Glycohaemoglobin, must not be used for other methods. In fact these programs have a specific software for the correspondent Biochemical Systems International products.
Screen-Master plus - User's guide
Biochemical Systems International s.r.l., via Galileo Ferraris, 220- Arezzo ITALY
Page 4 of 36
HOW TO USE THE MANUAL In order to better understand this manual, the instrument functions are explained in a schematic way, referring to examples. These examples are explained as follows: a. On the left side of the page and in italics, you can read what appears on the instrument display during the different stages of the analysis. b. On the right side you find a list of possible keys that may be pressed on the instrument. In the following table, you find the various symbols and their meaning when using this manual:
SYMBOLS
MEANING Warning or Caution advice: they are related to user’s and/or instrument safety
Enter analysis Number < >
Main menu
q [ENTER]
The operator shall push the key in brackets (in this example the key ENTER)
q [1/YES] o [0/NO]
The operator must press one of the key in brackets (in this example the key 1/YES or the key 0/NO). The instrument passes to the following operation.
q [ENTER] q [LIST]
The operator may push either key. Pushing the key in bold type permits to pass to the following operation shown on the manual. Pushing the other keys selects alternative functions.
q [0] q [1] q [ENTER]
Sequence of keys to be pushed. In the example it is necessary to push key 0, key 1 and key ENTER one after the other.
q [0, ......., 9, CL] q [OTHER KEY]
The operator shall enter a number by using keys 0 to 9 and key CL. The latter will clear the number when entering. The operator can push any key different from the previous ones
q [READ]
Screen-Master plus - User's guide
Biochemical Systems International s.r.l., via Galileo Ferraris, 220- Arezzo ITALY
Page 5 of 36
1. INSTRUMENT INSTALLATION 1.1
Unpacking the instrument
Check if the package is in perfect condition and with the original seals intact. If the package shows any serious damage, it may have suffered from improper handling: contact your dealer for instructions. The box should contain, besides this manual, the following items: 1) 2) 3) 4) 5) 6) 7) 8) 9)
the Screen Master Plus instrument; the instrument dusty-cover; the power cable; 2 rolls of printing paper; a spare 5W/12V halogen lamp already cabled; 2 spare 500mA fast-type fuses; a box with 100 1cm optical-path cuvettes; the Quality Control report; the warranty document;
Do not lose the original envelope and package since they are required in case of moving or shipping the instrument.
1.2
Instrument description
Figure 1 shows the main parts of the instrument:
DISPLAY
KEYBOARD
PRINTER COVER
INCUBATOR 37°C
READING SECTION
Figure 1: Global view of the instrument
Screen-Master plus - User's guide
Biochemical Systems International s.r.l., via Galileo Ferraris, 220- Arezzo ITALY
Page 6 of 36 FRONT SIDE Display.
It is back-illuminated liquid crystal type, consisting of two 16-character lines. Usually, the first line displays the main parameters of the analysis in progress (analysis number, item and K). The second line guides the operator in the analysis performance in an interactive way.
Printer.
It is a 20-column thermal type. It can be operated or not by the operator and can print both analysis results and parameters. The paper feed button is located in the back panel.
Incubator.
It consists of one aluminum unit set at a temperature of 37°C with 10 housings for both cylindrical and prismatic cuvettes, arranged on two rows of 5. Thermostating is obtained by means of semiconductor devices assuring a high accuracy. The thermostated unit has a further housing, separated from the previous ones, that serves as a reading cell.
Keyboard.
The keyboard (figure 2) is the device that permits to send the instrument both digital and alphanumeric data. The data entry is made easier by the emission of a sound when pushing a key. It consists of 16 keys, 10 digital and 8 functional. Two keys (1/YES and 0/NO) are both digital (1 and 0) and function (YES and NO) keys. The following table shows the function obtained when pushing each of the function keys.
Figure 2: Detail of the keyboard
Function keys
Action
[ENTER]: [LIST]: [STOP]: [READ]: [CL]: [1/YES]: [0/NO]: [•]/[PG]*
Enter Display or print of a list of parameters The operation in progress is stopped. (Always active key) Execution of photometric readings or parameter storage Digital value cancel when entering Acknowledgement Cancel Modification of analytic parameters
*This manual will show only the [PG] key. In the instruments where this key is not present, the key [.] shall be used.
Screen-Master plus - User's guide
Biochemical Systems International s.r.l., via Galileo Ferraris, 220- Arezzo ITALY
Page 7 of 36 PRINTER COVER
ON/OFF SWITCH
2 AC FUSES
SERIAL NUMBER
RS-232 CONNECTOR Figure 3: Back view of the instrument BACK SIDE In the back side of the instrument (Figure 3) you can find the following items: •
RS-232 D-type 9-pin male connector for connection with an external serial printer or PC through the serial port. You can use Biochemical Systems International Data Handling Software to connect the instrument to a PC: ask your dealer for further details.
•
ON/OFF switch
•
Fuse holder, containing number 2 fuses. Refer to technical features paragraph for fuses values.
1.3
First installation of the instrument
The instrument is for 220 V - 50 Hz (117 - 60Hz version is available upon request) power supply.
WARNING: make sure the chosen supply socket has a suitable earth connection, since it is required to assure user’s safety during instrument usage. •
Place the instrument on a solid, stable and vibration-free support. Avoid its placing near heat sources (e.g. heaters, ovens, under high power lamps), under direct sunlight, near strong electromagnetic sources (e.g. motors). The operational temperature range is 15-30Cº and humidity must be under 80%.
•
Before connecting the instrument to the power supply, make sure that it is switched off. In this case, the switch on the polysnap module in the backside of the instrument must be in the 0 position.
IMPORTANT: WAIT AT LEAST 15 MINUTES BEFORE EXECUTING ANY ANALYSIS, THUS ALLOWING THE INCUBATOR TO REACH AN OPTIMAL THERMAL STATUS.
Screen-Master plus - User's guide
Biochemical Systems International s.r.l., via Galileo Ferraris, 220- Arezzo ITALY
Page 8 of 36
1.4
Language selection and printer setting
The printer can print the following information: a. analytic results b. parameters of one analysis c. list of analysis names d. list of all analyses parameters It is possible to connect or disconnect the printer, set the number of white rows required between a printed row and the next one and moreover, select language: English, Italian, German or French. When the printer is connected, any information (a-d) can be printed upon request. The information a e b can be printed also in automatic mode. When the automatic mode is selected, the list of parameters is printed as soon as an analysis is selected and the results are printed at the end of each analysis. For the connection or disconnection of the printer and for the automation of the print of information a and b operate as follows: DISPLAY:
KEYBOARD:
Enter analysis number < >
q
[9]
Program Setup Enable print YES
q
[READ] to store.
q Program Setup Print meth. YES
q
q Program Setup Print result YES
q
q Program Setup Number rows 00 Program Setup Language
q q q
q [8] q [ENTER]
q
[1/YES] or [0/NO] [ENTER] to connect or disconnect the printer [READ] to store.
q
[1/YES] or [0/NO] [ENTER] to connect or disconnect the automatic printing of name and limit values of analysis [ENTER] to go on.
q
[1/YES] or [0/NO] [READ] to connect or disconnect the automatic analytic result print
q
[0,...,9] [ENTER] to set the number of white rows between a printed row and the next one. [1/YES] or [0/NO] to select the language [READ] to store
Program Setup Save (Yes/No)
q
[0/NO] to continue the printer entry.
q
[1/YES] to store the new entry.
Enter analysis number < >
Screen-Master plus - User's guide
Biochemical Systems International s.r.l., via Galileo Ferraris, 220- Arezzo ITALY
Page 9 of 36
1.5
Instrument maintenance
It’s recommended by Biochemical Systems International to follow these operations for a correct maintenance of the instrument: •
Use a cloth dampened with alcohol or detergent to clean the cover
•
Avoid dropping moisture and water in the reading hole, in the incubator and on the printer
•
Avoid dust and cover the instrument with its plastic cover when not in use.
•
Always use Biochemical Systems International original package to pack/ship the instrument.
•
If the instrument has to face a long inactivity period, store it in its original envelope and package between 0°C to 50 °C, avoiding moisture and wet places.
CAUTION: If the instrument is used differently and not as described above
by the producer, its integrity could be compromised.
Screen-Master plus - User's guide
Biochemical Systems International s.r.l., via Galileo Ferraris, 220- Arezzo ITALY
Page 10 of 36
2 2.1
PRINTING Print and display of the analyses name list
DISPLAY:
KEYBOARD:
Enter analysis number < >
q
[LIST]
The list of names of the first four analyses appears on the display. Example:
01PROT 02AMYL 03PHOS 04ALP
q q
q
[STOP] to stop the operation [ANY OTHER] to display the 4 subsequent names [LIST] to print the list of analyses names.
The instrument prints the list of items of analysis names. During printing you can push the key [STOP] to stop the operation.
2.2
Print of analytic parameters of one analysis
The parameters of an analysis can be printed by pushing the key [LIST] when one of the following messages appears on the display:
Insert blank Insert sample Insert standard 2.3
Print of results
The results of an analysis can be printed in the automatic mode (if it is active) at the end of the analysis; otherwise, they can be printed by pushing the key [LIST] when the result is visualized on the display. The result that is printed contains the following information (common to all analyses):
•
- Progressive number (1 to 9999)
•
- Measuring unit
•
- Value
• •
- Indication of exceeding the preset limit values. In case of a lower result than the lower limit value the letter L appears on the result right. In case of a higher result than the higher limit value the letter H appears on the result right. - Indication of a Fixed-time or kinetic analysis carried out with an instrument at a temperature different from 37°C. In this case the symbol * appears on the result right.
Screen-Master plus - User's guide
Biochemical Systems International s.r.l., via Galileo Ferraris, 220- Arezzo ITALY
Page 11 of 36
2.4
Print of all analyses parameters
It is possible to print the list of all stored analyses parameters as follows: DISPLAY:
KEYBOARD:
Enter analysis number < >
q
[9]
q [7] q [ENTER]
Print analysis first analys.
q
q
[0, ......., 9] [ENTER] to select the number of first analysis to print
Print analysis last analys.
Screen-Master plus - User's guide
q
q
[0, ......., 9] [ENTER] to select the number of last analysis to print
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3
EXECUTION OF ANALYSES
At power on the instrument sets the temperature of the thermostat and of the cuvette-holder space at 37°C.
IMPORTANT NOTES: •
AFTER POWER ON, WAIT AL LEAST 15 MINUTES BEFORE EXECUTING ANALYSES.
•
IF CUVETTES HAVE GRAINED FACES, INSERT THEM IN THE ANALYSER WITH A GRAINED FACE TOWARDS THE OPERATOR
WARNING:
3.1
ALWAYS WEAR GLOVES WHEN HANDLING CUVETTES, REAGENTS AND ALSO WHEN CLEANING THE INSTRUMENT
Absorbance readings
Plain absorbance readings can be determined with program and since absorbance is an absolute measurement it does not need any particular programming or calculation. You only need to select the right wavelength value for the desired absorbance measure. Proceed as follows: DISPLAY :
KEYBOARD :
Enter analysis
q
number < >
[0]
q [0] q [ENTER]
00 ABS PROGRAM
q
Filter WL 340
[1/YES] o [0/NO] wavelength
q [ENTER] to select the
00 ABS PROGRAM Insert Blank
q
[READ] to zero the analyser
q
[STOP] to stop the operation
00 ABS PROGRAM Abs. value XXXX
The absorbance value is continuously shown until [STOP] key is pressed. This method, applied to sample control solutions with known absorbance value at a wavelength supported by the instrument, can be used to check filters and lamp integrity or verify the instrument general accuracy. NOTE: DEFECTIVE FILTERS OR LAMP WILL LEAD TO UNCORRECT MEASURES
3.2
Selection of the analysis
When switching on the instrument the display shows:
Enter analysis number < > Enter the number corresponding (1 to 60) to the desired analysis from the keyboard and push [ENTER]. If you do not remember the number of the desired analysis, pushing the key [LIST] will show the list of analyses names and relevant numbers on the display. After the selection, if the automatic print of the analysis parameters was entered, they are printed. After the selection, the first display line shows the number of the analysis, the analysis name, the symbol K and the constant K value. Screen-Master plus - User's guide
Biochemical Systems International s.r.l., via Galileo Ferraris, 220- Arezzo ITALY
Page 13 of 36 Example:
DISPLAY:
KEYBOARD:
Enter analysis number < >
q
01 TPROT K 335 Insert blank
................................
3.3
[0]
q [1] q [ENTER]
Performance of the End-point type analysis with K
Select the desired analysis and proceed as follows. DISPLAY:
01 TPROT K 335 Insert blank
KEYBOARD:
q q q
q 01 TPROT K 335 Insert sample
q q q
q
[ENTER] to modify the progressive number of analysis [PG] to modify the parameters of the analysis. [LIST] to print the parameters of the analysis [READ] to read the blank cuvette [ENTER] to modify the progressive number of analysis [PG] to modify the parameters of the analysis. [LIST] to print the parameters of the analysis [READ] to read the sample cuvette
01 TPROT K 335 Temp 37 XXXX The analyser will wait XXXX seconds before testing the sample. After reading the sample, the result of the analysis is shown on the display and, if the automatic print of the result is actuated, also printed. The progressive sample number, the measuring unit and the digital value appear on the lower display line.
01 TPROT K 335 0001 g/dL 007.1
3.4
q
[LIST] to print the analysis result
q
[ANY OTHER] to go on
Performance of the whole blood analysis with Haematocrit correction
Analyses with Haematocrit correction (WBCHOL and WBTRIG) are End-Point reactions using a K-factor. The instrument reads the absorbance value of the samples in comparison with the absorbance value of R1 and thus determines the Haematocrit of the sample: this allows to express the results of the analysis in terms of plasmatic concentration of the analyte in each sample.
Screen-Master plus - User's guide
Biochemical Systems International s.r.l., via Galileo Ferraris, 220- Arezzo ITALY
Page 14 of 36 DISPLAY:
KEYBOARD:
25 WBCHOL K 00720 Insert blank
q q q
q 25 WBCHOL K 00720 Insert sample
q q q
[ENTER] to modify the progressive number [PG] to modify the parameters of the analysis [LIST] to print the parameters of the analysis [READ] to read the blank cuvette containing the R1 reagent only [ENTER] to modify the progressive number of the sample [PG] to modify the parameters of the analysis [LIST] to print the parameters of the analysis
q
[READ] to read the cuvette containing the blood sample
q
[READ] to obtain the result of the analysis
25 WBCHOL K 00720 Insert test 25 WBCHOL K 00720 Temp. 37 XXXX The analyser will wait XXXX seconds before testing the sample. After reading the sample, the result of the analysis is shown on the display and, if the automatic print of the result is actuated, also printed. The progressive sample number, the measuring unit and the digital value appear on the lower display line.
25 WBCHOL K 00720 0001 mg/dL 0121
3.5
q
[LIST] to print the analysis result
q
[ANY OTHER] to go on
Performance of the End-point type analysis with standard
Select the desired analysis and proceed as follows. DISPLAY:
01 TPROT K 335 Insert blank
KEYBOARD:
q q q
q 01 TPROT K 335 Calibration Y/N
01 TPROT K 335 Insert standard Screen-Master plus - User's guide
q
[ENTER] to modify the progressive number [PG] to modify the parameters of the analysis [LIST] to print the parameters of the analysis [READ] to read the blank cuvette
[0/NO] not to make calibration and use the K calculated by the instrument from previous calibration
q
[1/YES] to make calibration
q
[ENTER] to modify the progressive number
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Page 15 of 36
q q
q
[PG] to modify the parameters of the analysis [LIST] to print the parameters of the analysis [READ] to read the standard cuvette
The instrument determines the new K and shows its value on the display:
01 TPROT K 347 Insert sample
q q q
q
[ENTER] to modify the progressive number [PG] to modify the parameters of the analysis [LIST] to print the parameters of the analysis [READ] to read the sample cuvette
After reading the sample, the result of the analysis is shown on the display and, if the automatic print of the result is actuated, also printed. The progressive sample number, the measuring unit and the digital value appear on the lower display line.
01 TPROT K 335 0001 g/dL 007.1
3.6
q
[LIST] to print the analysis result
q
[OTHER KEY] to go on
Performance of the Glycohaemoglobin test
This analysis is an End-Point reaction with standard. Select the GLYC method and proceed as follows. DISPLAY:
KEYBOARD:
27 GLYC K 0103 Calibration Y/N
q
q 27 GLYC Calibra Insert blank
q q
[0/NO] to omit the calibration and use the K calculated by the instrument in its previous calibration [1/YES] to make calibration [ENTER] to modify the progressive number [PG] to modify the parameters of the analysis, especially the standard value (if necessary)
q
[LIST] to print the parameters of the analysis
q
[READ] to read the blank cuvette
q
[READ] to start the first incubation
27 GLYC Calibra Start I incub. 27 GLYC Calibra Temp OK XXXX XXXX is a timer which indicates the residual time (in seconds) for a complete lysis of the blood sample.
27 GLYC Calibra Insert total HGB
Screen-Master plus - User's guide
q
[READ] to read the Total Haemoglobin cuvette of the standard
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27 GLYC Calibra
q
Start II incub.
[READ] to start the second incubation
27 GLYC Calibra Temp OK XXXX The timer now indicates the residual contact-time between lysate and resin.
27 GLYC Calibra
q
Insert Glyc-HGB
[READ] to read the Glycohaemoglobin cuvette of the standard
The instrument calculates the new K and visualizes it on the display. After calibrating, the analyses of blood samples are performed in the same way as that of the standard. The MGLY program is similar to GLYC but the waiting times are not inserted. It can be used by an operator who wants to perform several analyses at the same time; in this case, he must regulate by himself the lysis-time and contact-time between lysate and resin.
3.7
Performance of the Multistandard type analysis
The programs between 51 and 60 are open and can also be used for multistandard-type analyses. In this case, the operator must enter in the program mode, select the MSD method and set the concentration values of the standards (up to 7) in an increasing order. DISPLAY:
KEYBOARD:
55 OPEN 1 Insert blank
q q q
[ENTER] to modify the progressive number [PG] to modify the parameters of the analysis [LIST] to print the parameters of the analysis
q
[READ] to read the blank cuvette
q
[READ] to read the first standard cuvette
q
[READ] to read the second standard cuvette
q
[READ] to read the third standard cuvette
q
[READ] to read the sample cuvette
55 OPEN 1 Insert standard 1 55 OPEN 1 Insert standard 2 55 OPEN 1 Insert standard 3 Suppose the standards are only 3 in this method.
55 OPEN 1 Insert sample
The instrument executes a linear interpolation between each pair of concentration values of the various standards. If the concentration sample is less than that of the lowest standard, LLLL is shown on the display. On the contrary, if the concentration of the sample is greater than that of the highest standard, on the display is shown HHHH: in this case, dilute the sample and repeat the analysis.
3.8
Performance of the Kinetic type analysis
Select the desired analysis and proceed as follows.
Screen-Master plus - User's guide
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Page 17 of 36 DISPLAY:
KEYBOARD:
02 AMYL K 3953 Insert sample
q q q
q
[ENTER] to modify the progressive number [PG] to modify the parameters of the analysis [LIST] to print the parameters of the analysis [READ] to read the sample cuvette
02 AMYL K 3953 Temp 37 XXXX XXXX is a timer that continuously indicates the residual time (in seconds) to achieve the analysis. When the timer indicates 0000, the sample analysis is at the end and the result is visualized on the display; if the automatic result print was actuated, this result is also printed. The progressive sample number, the measuring unit and the digital value appear on the lower display line.
02 AMYL K 3953 0001 U/L 047.1
3.9
q
[LIST] to print the analysis result
q
[OTHER KEY] to go on
Performance of the Fixed-time type analysis with K
Select the desired analysis and proceed as in the following example. DISPLAY:
KEYBOARD:
18 CREAT K 523 Insert sample
q q q
q
[ENTER] to modify the progressive number [PG] to modify the parameters of the analysis [LIST] to print the parameters of the analysis [READ] to read the sample cuvette
18 CREAT K 523 Temp 37 XXXX XXXX is a timer that continuously indicates the residual time (in seconds) to achieve the analysis. When the timer indicates 0000, the sample analysis is at the end and the result is visualized on the display; if the automatic result print was actuated, this result is also printed. The progressive number, the measuring unit and the digital value appear on the lower display line.
18 CREAT K 523 0001 mg/dL 002.5
q
[LIST] to print the analysis result
q
[OTHER KEY] to go on
3.10 Performance of the Fixed-time type analysis with standard Select the desired analysis and proceed as follows.
Screen-Master plus - User's guide
Biochemical Systems International s.r.l., via Galileo Ferraris, 220- Arezzo ITALY
Page 18 of 36 DISPLAY:
KEYBOARD:
18 CREAT K 512 Calibration Y/N
q
q 18 CREAT K 512 Insert standard
q q q
q
[0/NO] not to make calibration and use the K calculated by the instrument from previous calibration [1/YES] to make calibration [ENTER] to modify the progressive number [PG] to modify the parameters of the analysis [LIST] to print the parameters of the analysis [READ] to read the standard cuvette
18 CREAT K 523 Temp 37 XXXX XXXX is a timer that continuously indicates the residual time (in seconds) to achieve the calibration. When the timer indicates 0000, the calibration is at the end and the instrument determines the new K and shows its value on the display.
18 CREAT K 523 Insert sample
q q q
q
[ENTER] to modify the progressive number [PG] to modify the parameters of the analysis [LIST] to print the parameters of the analysis [READ] to read the sample cuvette
18 CREAT K 523 Temp 37 XXXX XXXX is a timer that continuously indicates the residual time (in seconds) to achieve the analysis. When the timer indicates 0000, the sample analysis is at the end and the result is visualized on the display; if the automatic result print was activated, this result is also printed. The progressive sample number, the measuring unit and the digital value appear on the lower display line.
18 CREAT K 523 0001 mg/dL 002.5
q
[LIST] to print the analysis result
q
[OTHER KEY] to go on
3.11 Read with incubator temperature lower then 37 °C. The analyses can even be performed at a temperature below 37 °C. In this case the message " No temp" will appear instead of "Temp OK". A star will be printed near the result of Fixed-time e Kin type analyses .
3.12 Modification of the progressive number The progressive number is a four digit number that is incremented automatically by 1 unit whenever an analysis is carried out. This number is displayed and printed as the first datum before the result measuring unit. This value can be modified when one of the following data is present on the second display line:
Insert blank Insert sample Insert standard and the key [ENTER] is pushed.
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Page 19 of 36
3.13 Waste processing Always follow the usual clinical laboratory rules to process the waste bottle content, exhaust reagents, used cuvettes and any other object that may be contaminated with organic and/or chemical fluids.
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Page 20 of 36
4
MODIFICATION OF THE ANALYSES PARAMETERS (Including K and Standard)
For parameters of an analysis we mean all information required by the instrument to carry out that analysis according to the preselected methods. The following table shows the parameter list. parameter
abbreviations
values
Name
Item that identifies the analysis to be carried out. One of the 42 different items stored in the instrument can be selected.
Method
EP, KIN, FXT, MSD
K Standard
S
Filter
WL
Delay
Reaction time
Identifies the type of analytic method: EP = End-point method KIN = Kinetic method FXT = Fixed-time method MSD = Multistandard method
0-9999
Value of the analytic constant
0-9999
Value of standard concentration
340,405,505,546,630
Units Decimal point
description
Value of the wavelength used for analysis Measuring unit
DP, Decimal P. DLY
React t, Rt, R. t
Repeat blank
0-3 0-999
0-999
YES/NO
Number of decimal points Wait time between the key pushing [READ]and the first reading in KIN and FXT methods or wait time first reading sample in EP methods Time between the first and the last reading in KIN and FXT methods. In methods EP, when (YES) is enabled it requires the blank reading for each sample. Otherwise (NO), for the reading of a set of samples, the analyser requires only one blank.
Low limit
Lval, L
0-9999
Lower limit value. When (=0) is entered and the analytic result is lower than this limit, during printing the letter L appears near the result.
High limit
Hval, H
0-9999
Higher limit value. When (≠ 0) is entered and the analytic result is higher than this limit, during printing the letter H appears near the result.
Screen-Master plus - User's guide
Biochemical Systems International s.r.l., via Galileo Ferraris, 220- Arezzo ITALY
Page 21 of 36 The following table indicates which are the required parameters according to the type of analysis to be carried out (Endpoint, kinetic, Fixed-time).
End-point (EP) with K
End-point (EP) with standard
Kinetic (KIN)
Fixed-time (FXT) with K
Fixed-time (FXT) with standard
Method
‡
‡
‡
‡
‡
K
‡
‡
‡
Method
Parameter
‡
Standard
‡
Filter
‡
‡
‡
‡
‡
Units
‡
‡
‡
‡
‡
Decimal point
‡
‡
‡
‡
‡
Delay
‡
‡
‡
‡
‡
‡
‡
‡
Reaction time Repeat blank
‡
‡
Low limit
‡
‡
‡
‡
‡
High limit
‡
‡
‡
‡
‡
The parameters of an analysis can be modified when one of the following messages appears on the display:
Insert blank Insert sample Insert standard Insert test and the key [PG] is pushed. The operations required to modify one or more parameters of an End-point, kinetic and Fixedtime analysis are exemplified here below. During these operations the values to be modified will appear on the display in two different ways: 1) value with active cursor (underlining line). To modify the value it is necessary to use digital keys to enter the desired value. After entering the value, push the key [ENTER]. 2) value without cursor (not underlined). To modify the value it is sufficient to use the cursor keys [1/YES] and [0/NO] to select one of the possible preset values that appear on the display. When the desired message appears on the display, push the key [ENTER]. At any time, pushing the key [ENTER] causes the passage from the present parameter to the following one; pushing the key [READ] allows the storage of parameter values and pushing the key [STOP] causes the operation stop without storing the parameter values.
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4.1
Modification of the parameters of an End-point analysis with K
Proceed as indicated in the following example: DISPLAY:
KEYBOARD:
Enter analysis number < > 01 TPROT K 335 Insert blank
01 TPROT K 335 Method EP
q q q q
q
q
q
q
q 01 TPROT K 335 Units g/dL
q
q 01 TPROT K 335 Decimal p. 1
q
q Screen-Master plus - User's guide
[READ] to read the blank cuvette.
[READ] to store values
q 01 TPROT K 335 Filter WL 546
[LIST] to print the parameters of the analysis.
q
q 01 TPROT K 335 New name TPROT
[ENTER] to modify the progressive number
[PG] to modify the parameters of the analysis
q 01 TPROT K 335 New K
q [1] q [ENTER]
q q 01 TPROT K 335 Use standard YES/NO
[0]
q
[1/YES] o [0/NO] [ENTER] to select the type of methods (EP, KIN, FXT). Select type EP.
[READ] to store values
q
[1/YES] o [0/NO] [ENTER] to use or not the standard. Select NO. [READ] to store values
q
[0, ......., 9] [ENTER] to enter the value of the new K. This value shall be entered without any decimal point (ex.K=1.25; enter: K=1250). Enter 335
[READ] to store values [1/YES] o [0/NO] Select TPROT.
q [ENTER] to select the name.
[READ] to store values [1/YES] o [0/NO] Select 546.
q [ENTER] to select the wavelength.
[READ] to store values [1/YES] o [0/NO] unit. Select g/dL.
q [ENTER] to select the measuring
[READ] to store values
q
[1/YES] o [0/NO] [ENTER] to select the number of decimal points of the result. Select 1.
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01 TPROT K 335 Repeat blank YES
q
q 01 TPROT K 335 Low limit 40
q
q 01 TPROT K 335 High limit 95
q
q 01 TPROT K 335 Delay 300
q
q 01 TPROT K 335 Save (YES/NO)
q
q
[READ] to store values
q
[1/YES] o [0/NO] [ENTER]. Reply YES when the blank repetition for every sample is required. Select NO.
[READ] to store values
q
[0, ......., 9] [ENTER] to enter the lower threshold value. Enter the desired value (ex. 40). [READ] to store values
q
[0, ......., 9] [READ] to enter the higher threshold value. Enter the desired value (ex.95). [READ] to store values
q
[0, ......., 9] [ENTER] to enter the waiting time, in seconds . Enter the desired value (ex. 300). [0/NO] to make other value modifications, without storing the previous ones. [1/YES] to store values
Enter analysis number < > At this point it is possible to carry out the analysis with the new parameters. During any of above stages, pushing the key [STOP] stops the operation of parameter modification, without storing the new values.
4.2
Modification of the parameters of an End-point analysis with standard
To modify the parameters of an End-point analysis with the standard proceed as indicated in the following example:
DISPLAY:
KEYBOARD:
Enter analysis number < > 01 TPROT K 0000 Insert blank
q q q q
q
Screen-Master plus - User's guide
[0]
q [1] q [ENTER]
[ENTER] to modify the progressive number [LIST] to print the parameters of the analysis. [READ] to read the blank cuvette. [PG] to modify the parameters of the analysis
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Page 24 of 36
01 TPROT K 0000 Method EP
q
q 01 TPROT K 0000 Use standard YES/NO 01 TPROT K 0000 Standard val 705
q
[1/YES] o [0/NO] [ENTER] to select the type of methods (EP, KIN, FXT). Select type EP.
q
[READ] to store values
q
[1/YES]
q
[READ] to store values
q 01 TPROT K 0000 New name TPROT
[READ] to store values
q
q [ENTER] to use the standard. q
[0, ......., 9] [ENTER] to enter the concentration value of the new standard. This value shall be entered without any decimal point (ex. Std = 2.26; enter. val= 2260). Enter 705 [READ] to store values
q
[1/YES] o [0/NO] Select TPROT.
01 TPROT K 0000 Filter WL 546
q
q 01 TPROT K 0000 Units g/dL
q
q 01 TPROT K 0000 Decimal p. 1
q
q 01 TPROT K 0000 Repeat blank YES
q
q 01 TPROT K 0000 Low limit 40
q
q 01 TPROT K 0000 High limit 95
q
q
Screen-Master plus - User's guide
q [ENTER] to select the name.
[READ] to store values
q
[1/YES] o [0/NO] [ENTER] to select the wavelength. Select 546. [READ] to store values [1/YES] o [0/NO] unit. Select g/dL.
q [ENTER] to select the measuring
[READ] to store values
q
[1/YES] o [0/NO] [ENTER] to select the number of decimal points of the result. Select 1. [READ] to store values
q
[1/YES] o [0/NO] [ENTER]. Replay YES when the Blank repetition for every sample is required. Select NO. [READ] to store values
q
[0, ......., 9] [ENTER] to enter the lower threshold value. Enter the desired value (ex. 40). [READ] to store values
q
[0, ......., 9] [ENTER] to enter the higher threshold value. Enter the desired value (ex. 95).
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01 TPROT K 0000 Delay 300
q
q 01 TPROT K 0000 Save (YES/NO)
q
q
[READ] to store values
q
[0, ......., 9] [ENTER] To enter the waiting time, in seconds. Enter the desired value (ex. 300). [0/NO] . to make other value modification, without storing the previous one. [1/YES] to store values
Enter analysis number < > At this point it is possible to carry out the analysis with the new parameters. During any of above stages, pushing the key [STOP] stops the operation of parameter modification, without storing the new values.
4.3
Modification of the parameters of a kinetic analysis
To modify the parameters of a kinetic analysis proceed as follows: DISPLAY:
KEYBOARD:
Enter analysis number < > 02 ALP K 3027 Insert sample
02 ALP K 335 Method KIN
q [0] q [2] q [ENTER] q q q q
[READ] to store values
q
q
q
Screen-Master plus - User's guide
[READ] to read the blank cuvette. [PG] to modify the parameters of the analysis
q 02 ALP K 3027 New name ALP
[LIST] to print the parameters of the analysis.
q q 02 ALP K 3027 New K
[ENTER] to modify the progressive number
q
[1/YES] o [0/NO] [ENTER] to select the type of methods (EP, KIN, FXT). Select type KIN. [READ] to store values
q
[0, ......., 9] [ENTER] to enter the value of the new K. This value shall be entered without any decimal point (ex.K=1.25; enter: K=1250). Enter 3027
[READ] to store values [1/YES] o [0/NO] Select ALP.
q [ENTER] to select the name.
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Page 26 of 36
02 ALP K 3027 Filter WL 405
q
q 02 ALP K 3027 Units U/L
q
q 02 ALP K 3027 Decimal p. 0
q
q 02 ALP K 3027 Delay 30
q
q 02 ALP K 3027 React t 120
q
q 02 ALP K 3027 Low limit 40
q
q 02 ALP K 3027 High limit 95
q
q 02 ALP K 3027 Save (YES/NO)
q
q
[READ] to store values
q
[1/YES] o [0/NO] [ENTER] to select the wavelength. Select 405. [READ] to store values [1/YES] o [0/NO] unit. Select U/L.
q [ENTER] to select the measuring
[READ] to store values
q
[1/YES] o [0/NO] [ENTER] to select the number of decimal points of the result. Select 0. [READ] to store values
q
[0, ......., 9] [ENTER] to set the delay time in seconds. Enter the desired value (ex.30). [READ] to store values
q
[0, ......., 9] [ENTER] to set the kinetic reading time in seconds. Enter the desired value (ex.120). [READ] to store values
q
[0, ......., 9] [ENTER] to enter the lower threshold value. Enter the desired value (ex. 40). [READ] to store values
q
[0, ......., 9] [ENTER] to enter the higher threshold value. Enter the desired value (ex.95). [0/NO] to make other value modifications, without storing the previous ones. [1/YES] to store values
Enter analysis number < > At this point it is possible to carry out the analysis with the new parameters. During any of above stages, pushing the key [STOP] stops the operation of parameter modification, without storing the new values.
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Page 27 of 36
4.4
Modification of the parameters of a Fixed-time analysis with K
To modify the parameters of a Fixed-time analysis with K proceed as follows: DISPLAY:
KEYBOARD:
Enter analysis number < >
18 CREAT K 0000 Insert sample
18 CREAT K 0000 Method FXT
q [1] q [8] q [ENTER] q q q
18 CREAT K 0000 New K
q
[READ] to store values.
[READ] to store values
q
[0/NO]
q
[READ] to store values
q
q
q 18 CREAT K 0000 Units mg/dL
q
q 18 CREAT K 0000 Decimal p. 1
q
q
Screen-Master plus - User's guide
q
[1/YES] o [0/NO] [ENTER] to select the type of methods (EP, KIN, FXT). Select type FXT.
q
q 18 CREAT K 0000 Filter WL 505
[READ] to read the standard cuvette. [PG] to modify the parameters of the analysis
q 18 CREAT K 0000 New name ALP
[LIST] to print the parameters of the analysis.
q q 18 CREAT K 0000 Use standard YES/NO
[ENTER] to modify the progressive number
q [ENTER] to use K q
[0, ......., 9] [ENTER] to enter the value of the new K. This value shall be entered without any decimal point (ex.K=1.25; enter: K=1250). Enter 3300 [READ] to store values [1/YES] o [0/NO] Select CREAT
q [ENTER] to select the name.
[READ] to store values
q
[1/YES] o [0/NO] [ENTER] to select the wavelength. Select 505 [READ] to store values
q
[1/YES] o [0/NO] [ENTER] to select the measuring unit. Select mg/dL. [READ] to store values
q
[1/YES] o [0/NO] [ENTER] to select the number of decimal points of the result. Select 1.
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18 CREAT K 0000 Delay 10
q
q 18 CREAT K 0000 React t 120
q
q 18 CREAT K 0000 Low limit 05
q
q 18 CREAT K 0000 High limit 20
q
q 18 CREAT K 0000 Save (YES/NO)
q
q
[READ] to store values
q
[0, ......., 9] [ENTER] to set the delay time in seconds. Enter the desired value (ex.10). [READ] to store values
q
[0, ...., 9] [ENTER] to set the kinetic reading time in seconds. Enter the desired value (ex.120). [READ] to store values
q
[0, ......., 9] [ENTER] to enter the lower threshold value. Enter the desired value (ex. 05). [READ] to store values
q
[0, ......., 9] [ENTER] to enter the higher threshold value. Enter the desired value (ex. 20). [0/NO] to make other value modifications, without storing the previous ones. [1/YES] to store values
Enter analysis number < > At this point it is possible to carry out the analysis with the new parameters. During any of above stages, pushing the key [STOP] stops the operation of parameter modification, without storing the new values.
4.5
Modification of the parameters of a Fixed-time analysis with standard
To modify the parameters of a Fixed-time analysis with standard proceed as follows: DISPLAY:
KEYBOARD:
Enter analysis number < >
q [1] q [8] q [ENTER]
18 CREAT K 0000 Calibration Y/N
q
q
Screen-Master plus - User's guide
[0/NO] not to make the calibration and use the K calculated by the instrument from previous calibration [1/YES] to make calibration
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Page 29 of 36
18 CREAT K 0000 Insert standard
18 CREAT K 0000 Method FXT
q q q
18 CREAT K 0000 Standard val
q
[READ] to store values.
[READ] to store values
q
[1/YES]
q
[READ] to store values
q
q
q 18 CREAT K 0000 Units mg/dL
q
q 18 CREAT K 0000 Decimal p. 1
q
q 18 CREAT K 0000 Delay 10
q
q 18 CREAT K 0000 React t 120
q
q
Screen-Master plus - User's guide
q
[1/YES] o [0/NO] [ENTER] to select the type of methods (EP, KIN, FXT). Select type FXT.
q
q 18 CREAT K 0000 Filter WL 505
[READ] to read the standard cuvette. [PG] to modify the parameters of the analysis
q 18 CREAT K 0000 New name ALP
[LIST] to print the parameters of the analysis.
q q 18 CREAT K 0000 Use standard YES/NO
[ENTER] to modify the progressive number
q [ENTER] to use standard. q
[0, ..., 9] [ENTER] to enter the value of the new standard. This value shall be entered without any decimal point (ex.Std=1.25; enter:val=1250).Enter 2000. [READ] to store values [1/YES] o [0/NO] Select CREAT.
q [ENTER] to select the name.
[READ] to store values
q
[1/YES] o [0/NO] [ENTER] to select the wavelength. Select 505. [READ] to store values
q
[1/YES] o [0/NO] [ENTER] to select the measuring unit. Select mg/dL. [READ] to store values
q
[1/YES] o [0/NO] [ENTER] to select the number of decimal points of the result. Select 1. [READ] to store values
q
[0, ......., 9] [ENTER] to set the delay time in seconds. Enter the desired value (ex. 10). [READ] to store values
q
[0, ......., 9] [ENTER] to set the kinetic reading time in seconds. Enter the desired value (ex.120).
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Page 30 of 36
18 CREAT K 0000 Low limit 05
q
q 18 CREAT K 0000 High limit 20
q
q 18 CREAT K 0000 Save (YES/NO)
q
q
[READ] to store values
q
[0, ......., 9] [ENTER] to enter the lower threshold value. Enter the desired value (ex. 05). [READ] to store values
q
[0, ......., 9] [ENTER] to enter the higher threshold value. Enter the desired value (ex. 20). [0/NO] to make other value modifications, without storing the previous ones. [1/YES] to store values
Enter analysis number < > At this point it is possible to carry out the analysis with the new parameters. During any of above stages, pushing the key [STOP] stops the operation of parameter modification, without storing the new values.
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Page 31 of 36
5
CALCULATION PROCEDURES TAKEN BY SCREEN-MASTER PLUS
a) End-point analysis The instrument measures the sample ABSORBANCE, multiplies it by a factor (K) and displays the results directly in the programmed concentration units. The calculation factor can be entered directly or obtained from the instrument according to the standard. In this case the factor is obtained from the known relation: K = C std ABSstd
Cstd = known concentration value of the used standard ABSstd = measured standard absorbance
The factor value (entered or computed) is used for the calculation of sample concentration according to the relation: Csample = ABSsample x K
Csample = Sample concentration ABSsample = Sample Absorbance
b) Kinetic analysis In a kinetic analysis the reaction speed is reached by taking a set of measures of sample ABSORBANCE. The instrument takes the first reading after a preset wait time (delay). Then it takes four readings at intervals corresponding to 1/4 of the total preset analysis time (reaction time). Then, the instrument calculates the ABSORBANCE difference between one measure and the previous one ( ABS) and determines the average of the four differences, referred to 1 minute (∆ ABS/min.). Multiplying the ∆ ABS /min by the preset factor (K), the instrument calculates the sample activity. Therefore, the following reaction is valid: Enzymatic activity (U/L) = ∆ ABS/min. x K Remember K, usually indicated by the reagent manufacturer, can be obtained from the following formula: FACTOR = Vtot . 1000 Vsample . t.e.s
Vtot Vsample= t e s
= Total volume of the reaction mixture Sample volume = Time (in minutes) = Molar extinction coefficient of chromogen = Cuvette thickness (in cm.)
c) Fixed-time analysis In this operating mode the instrument takes 2 ABS measures on the sample being examined: - The first one after a delay time after pushing the key [READ] - The second one after a reaction time from the first one. The instrument calculates the Absorbance difference between both readings ( ABS). The concentration calculation is made by multiplying the ABS by a suitable K. This K can be: 1) entered directly by the operator (Fixed-time with K) 2) calculated by the instrument according to a standard (Fixed-time with standard) Therefore, the calculation procedure is similar to the one used for End-point analyses.
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Page 32 of 36
6
TECHNICAL FEATURES
Photometric system Light source: Spectral field: Filter change: Filters: Detector: Cuvettes type:
5 W long-life iodine incandescent lamp 320 to 690 nm. automatic 340, 405, 505, 546, 630 nm.; 8 nm. pass-band solid state device 1cm optical path, square or cylindrical
Thermostating Heating element: Temperature: Temperature accuracy: Stabilization period: Thermostatic unit:
semiconductor 37°C ±0.2°C 10-20 min. 10 places
Measuring system: Reset: Measuring range: Photometric linearity: Photometric accuracy: Photometric precision: Accuracy: Drift: Reagent volume: Methods:
automatic -0.200 to +2,000 OD 1% ±1% from 0 to 2,000 O. D. CV < 1% @ 2.0 OD ±1 digit lower than 0.005 O. D. per hour 1 ml (minimum) End Point, Kinetic, Fixed Time, Multistandard, Differential
Data display and programming: Keyboard: Display: Thermal printer: Printer paper roll: Serial output Power supply: Size: Weight:
with 11 digital keys + 5 function keys back-illuminated liquid crystal alpha-numeric, with 32 characters 20 columns thermal type, 57mm wide, 44mm roll diameter. RS-232 standard 220 V, 50 Hz; 117 V, 60 Hz; 40 VA cm. 36x33x18 8 kg.
Serial transmission of data: The serial output, standard type RS-232, uses the following transmission parameters: 9600, N, 8, 1. Serial connection: The output connector, 9-pole standard type, is located on the instrument back. The connections are as follows: pin 2: pin 3: pin 5:
input output reference signal
The pin number of 9-pole connector is the same of the personal computer IBM type or IBM compatible.
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Page 33 of 36
7
MESSAGES AND ERROR SIGNALS
The following table shows the error messages related to issues that the user can normally solve himself: if the problem persists, or a problem not listed below arise, contact your dealer. Excluding the main plug fuses, the instrument has no user serviceable parts: only trained technicians are allowed to service the instrument. An unauthorized action on the instrument may invalidate its safety and features, beside void the warranty. MESSAGE
DESCRIPTION
POSSIBLE OPERATIONS
Out of range
The sample ABSORBANCE is too high or the reading is impossible due to instrument malfunction Error when printing
Push [READ] the read can be repeated.
Printer error Temp 37 No temp Filter selection Filter error Wait time XXX ---
LLLL HHHH
The temperature switch is set at 37°C. The temperature switch did not yet reach the temperature of 37°C The instrument is positioning itself on the selected filter Error in the filter selection XXXX = time (in seconds) required to achieve the analysis. In a KIN or FXT analysis, the absorbancevariation of the sample is too high during the first time-interval (Delay). In an MSD analysis, the sample concentration is less than that of the lowest standard In an MSD analysis, the sample concentration is greater than that of the highest standard
Push [STOP] to disable the printer or any other key to try again Wait* Wait Push [STOP] to stop the filter selection, any other key to try again. Wait or push [STOP] to stop the analysis Dilute the sample.
The sample concentration is lower than the minimum range of the method. Dilute the sample.
*This message appears only during kinetic or Fixed-time analyses. The operator can go on; the analysis will be carried out, but when printing, an asterisk will appear on the result right.
NOTE: In case of suspect maflunctioning of the instrument, we recommend to check the instrument with coloured solution or control serum of known value.
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Page 34 of 36
8
APPENDIX CONVERSION FACTORS OF ENZYMATIC ACTIVITIES (I.U.) AT DIFFERENT TEMPERATURES ENZIMA from:
25°C
30°C
37°C
at:
25°C 30°C 37°C
1 1.39 2.01
0.72 1 1.45
0.50 0.69 1
at:
25°C 30°C 37°C
1 1.29 1.74
0.77 1 1.35
0.57 0.74 1
at:
25°C 30°C 37°C
1 1.23 1.53
0.81 1 1.24
0.65 0.81 1
at:
25°C 30°C 37°C
1 1.39 2.14
0.72 1 1.54
0.47 0.65 1
at:
25°C 30°C 37°C
1 1.44 2.40
0.69 1 1.67
0.42 0.60 1
at:
25°C 30°C 37°C
1 1.37 1.79
0.73 1 1.31
0.56 0.77 1
at:
25°C 30°C 37°C
1 1.44 2.40
0.69 1 1.67
0.42 0.60 1
ALAT (GPT)
ALP
a-AMYL
ASAT (GOT)
CK
g-GT
LDH
EXTINCTION COEFFICIENTS ( cm²/mole x 106 ) WAVELENGTH (nm)
334
340
365
NADH2
6.18
6.3
1.33
NADPH2
6.18
6.3
1.33
405
505
CHROMOGEN
p-nitrophenol quinone imine
Screen-Master plus - User's guide
18.8 6.89
Biochemical Systems International s.r.l., via Galileo Ferraris, 220- Arezzo ITALY
Page 35 of 36
DICHIARAZIONE DI CONFORMITÀ CE EC STATEMENT OF COMPLIANCE DECLARATION DE CONFORMITE CE EG-KONFORMITÄTSERKLÄRUNG
Fabbricante: (Producer/Producteur/Hersteller)
Indirizzo:
Biochemical Systems International S.r.l. Via G. Ferraris, 220 - 52100 Arezzo - ITALY
(Address/Adresse/Anschrift)
Dichiara che l’apparecchiatura: Hereby states that the device known as: Déclare que l’appareil : Erklärt, daß das nachfolgend aufgeführte Gerät :
MODELLO
: Screen Master Plus
(MODEL/MODEL/MODELL)
È conforme alle seguenti direttive CE: 73/23CE, 89/336CE, 92/31CE, 93/68CE, 98/79/CE, come modificate e recepite dalla legislazione italiana The machinery meets the requirements set by the following EEC Directives:
DIRECTIVES 73/23EC, 89/336EC, 92/31CE, 93/68CE, 98/79/CE AS AMENDED AND IMPLEMENTED UNDER ITALIAN LAW L’APPAREIL EST CONFORME AUX DIRECTIVES CE SUIVANTES: DIRECTIVE 73/23EC, 89/336EC, 92/31CE, 93/68CE, 98/79/CE TELLE QUE MODIFIEE ET ACCUEILLIE FORMELLEMENT PAR LA LEGISLATION ITALIENNE. IM ENTSPRICHT DAS GERÄT DEN FOLGENDEN EG-RICHTLINIEN: EG RICHTLINIE 73/23EC, 89/336EC, 92/31CE, 93/68CE, 98/79/CE WIE VON DER ITALIENISCHEN RECHTSPRECHUNG MODIFIZIERT UND AUFGEFAßT
Sono state applicate le seguenti Norme Nazionali, che traspongono le Norme Armonizzate CE: The following national standards and technical specifications, conforming to EEC Harmonized Regulations, were followed: Les normes nationales transposant les normes harmonisées CE qui ont été appliquées sont les suivantes: Folgende nationale Normen wurden angewandt, die den vereinheitlichten EG-Normen entsprechen:
EN 61000-6-3 (2002/10), EN 55011 (1999/05), EN 55022 (1999/06) Class B
CEI EN 61000-3-2 (2002/04), CEI EN 61000-3-3 (1977/06) EN 61010-2-101 (2002/01)
Arezzo, 31 Ottobre 2003
……………………………… Dr. Oliviero Giusti
Screen-Master plus - User's guide
Biochemical Systems International s.r.l., via Galileo Ferraris, 220- Arezzo ITALY
Page 36 of 36 Reviewed: 26/03/04 Author: Biochemical Systems International
Screen-Master plus - User's guide
Biochemical Systems International s.r.l., via Galileo Ferraris, 220- Arezzo ITALY
