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Fully Automated Urine Particle Analyzer
UF-4000 Troubleshooting
Read this manual when you encounter a problem, and to perform instrument maintenance. The explanations in this manual assume that you have already read "General Information".
The following manuals are provided as Instructions for Use: • General Information • Basic Operation • Troubleshooting
KOBE, JAPAN
© SYSMEX CORPORATION
Code No. AY565820 en-eu PRINTED IN JAPAN Date of issue or revision: 03/2021 Software Version: Ver.1 onwards
Revision History
Revision History 05/2016 Initial issue Software version: Ver.1 08/2016 Software version: Ver.1 06/2017 Software version: Ver.1 07/2017 Software version: Ver.1 12/2017 Software version: Ver.1 02/2018 Software version: Ver.1 05/2018 Software version: Ver.1 09/2018 Software version: Ver.1 02/2019 Software version: Ver.1 08/2019 Software version: Ver.1 03/2020 Software version: Ver.1 04/2020 Software version: Ver.1 03/2021 Software version: Ver.1 Updated manuals: Basic Operation, General Infomation
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Revision History
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Table of Contents
Table of Contents Revision History
3
Chapter 1
7
1.1 1.2 1.3 1.4
Error message list (in alphabetical order) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 [ErrorHelp] dialog box . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 Causes of errors and corrective actions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 Remedial actions against U-RDU failure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Chapter 2 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 2.9 2.10 2.11 2.12 2.13 2.14 2.15 2.16
Maintenance
63
List of maintenance tasks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [Maintenance Menu] screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Checking the status of the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Checking the operation count (counter) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Performing a background check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Performing cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Rinsing the flowcell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Performing instrument operation tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Replenishing rinse water . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cleaning the rinse bottle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Checking the volume of fluid and removing fluid in the trap chamber . . . . . . . Disposing of waste fluid in the waste tank . . . . . . . . . . . . . . . . . . . . . . . . . . . . Replacing reagents. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Rinsing/replacing the sample filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Replacing the fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Maintenance inspection checklist . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Chapter 3 3.1 3.2
Troubleshooting
Checking the Logs
63 64 65 67 68 69 70 72 77 78 79 81 82 87 91 95
97
Logs and how to use them . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97 Log screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100
Index
107
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Table of Contents
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Chapter 1
Troubleshooting
Chapter 1 Troubleshooting 1.1
Error message list (in alphabetical order)
The following is an alphabetical list of error messages displayed in the instrument.
Note: Contact your local Sysmex service representative in the following situations, as the instrument requires repair or replacement of components: • An error message is displayed that is marked with (Telephone mark) in the error message list • The error is not cleared or the error reoccurs even after taking the described remedial action.
☎
Error message Numerics
A
☎ B
☎
Action
0.05MPa Pressure Error
15
0.24MPa Pressure Error
14
Abnormaly high RBC
41
Abnormal Pressure Loss
13
Air Bubble in UF-Fluorocell CR Line
24
Air Bubble in UF-Fluorocell SF Line
24
Back Exhaust Fan Error
33
Background Error(Body Fluid)
41
Background Error(Urine)
41
BACT High
41
Barcode Reader Communication Error
39
Barcode Reader Position Error
39
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Error message
Action
Cannot recognize UF-Fluorocell CR information
28
Cannot recognize UF-Fluorocell SF information
28
Charging Pump Error
32
Conductivity Analysis Chamber Temp High
22
Conductivity Analysis Chamber Temp Low
22
Conductivity Analysis Chamber Thermistor Error
23
Control Material Expired Error
40
Conveyor Communication Error
40
CR Preheating Unit Temp High
22
CR Preheating Unit Temp Low
22
CR Preheating Unit Thermistor Error
23
CR Reaction Chamber Temp High
21
CR Reaction Chamber Temp Low
21
CR Reaction Chamber Thermistor Error
23
Detector Base Chamber Temp High
22
Detector Base Chamber Temp Low
22
Detector Base Chamber Thermistor Error
23
Detector cover is open
43
Dispensing Pump Error
32
Dispensing Unit Left/Right Mechanism Motor Error
32
Dispensing Unit Probe Crash
32
Dispensing Unit Up/Down Mechanism Motor Error
32
E
Execute the shutdown
49
F
Failed to read sample number
39
Feed-in Table Stopper Position Error 1
48
Feed-in Table Stopper Position Error 2
48
Front cover is open
43
Guide Liquid Chamber Thermistor Error
23
H
Host computer Communication Error
40
L
L-J Control Error
40
L-J Control Error(internal QC)
40
Laser Output Error
33
Laser Radiation Error
33
C
☎
☎ ☎ D
☎
G
☎
☎ ☎
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Error message
Troubleshooting
Action
Mixing Motor(CR) Speed Error
32
Mixing Motor(SF) Speed Error
32
Mixing/Aspiration Unit Forward/Back Mechanism Motor Error
32
Mixing/Aspiration Unit Probe Crash
32
Mixing/Aspiration Unit Up/Down Mechanism Motor Error
32
N
No Rinse Water
30
O
Out of UF-CELLPACK CR
25
Out of UF-CELLPACK SF
25
Out of UF-CELLSHEATH
25
Out of UF-Fluorocell CR
28
Out of UF-Fluorocell SF
28
Photomultiplier Sensor Chamber Temp High
21
Photomultiplier Sensor Chamber Temp Low
21
Photomultiplier Sensor Chamber Thermistor Error
23
Positive ID Check Error
39
Power Unit Fan Error
33
Power was turned OFF without executing shutdown
49
Rack Exclusion Error
48
Rack Feed-in Error
47
Rack Feed-in Home Position Error
47
Rack Feed-out Error
47
Rack Feed-out Position Error
47
Rack is full
48
Rack is not placed
48
Rack Move Error(Back Belt)
47
Rack Move Error(Front Belt)
47
Rack Move Mechanism Initialization Error(Back Belt)
47
Rack Move Mechanism Initialization Error(Front Belt)
47
Replace the dispensing pump
43
Replace the Seal of the charging pump
43
Replace the Seal of the urine pump
43
Replace the sheath syringe
43
M
P
☎ ☎ R
☎ ☎ ☎ ☎
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Error message R
☎ S
☎ ☎
☎ ☎
☎
T
☎ ☎
Action
RFID Communication Error
40
Room temperature is high
23
Room temperature is low
23
Room Temperature Thermistor Error
23
Sampler Belt Error
48
Sampler driver error
49
Sampler Unit Fan Error
33
Sampling Error
41
SF Preheating Unit Temp High
22
SF Preheating Unit Temp Low
22
SF Preheating Unit Thermistor Error
23
SF Reaction Chamber Temp High
21
SF Reaction Chamber Temp Low
21
SF Reaction Chamber Thermistor Error
23
Sheath Fluid Temp High
23
Sheath Fluid Temp Low
23
Sheath Pressure Error
13
Sheath Syringe Error
32
Sheath Temperature Chamber Temp High
21
Sheath Temperature Chamber Temp Low
21
Sheath Temperature Chamber Thermistor Error
23
Short Sample(Dispensing)
34
Short Sample(Mixing)
34
Substrate Cooling Fan Error
33
Temperature Stabilizing Error
23
The service life of the laser has nearly expired.
33
Tube remains in tube holder
39
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Error message
Troubleshooting
Action
UF-CELLSHEATH Aspiration Error(FCM)
24
UF-CELLSHEATH Aspiration Error(Rinse)
24
UF-CELLPACK CR has expired
25
UF-CELLPACK SF has expired
25
UF-CELLSHEATH has expired
25
UF-Fluorocell CR has already been used
44
UF-Fluorocell CR has expired
28
UF-Fluorocell CR is not installed
44
UF-Fluorocell CR reagent cover is open
43
UF-Fluorocell CR replaced during operation
44
UF-Fluorocell CR RFID tag error
44
UF-Fluorocell SF has already been used
44
UF-Fluorocell SF has expired
28
UF-Fluorocell SF is not installed
44
UF-Fluorocell SF reagent cover is open
43
UF-Fluorocell SF replaced during operation
44
UF-Fluorocell SF RFID tag error
44
Urine Pump Error
32
V
Vacuum Pressure Error
17
W
Waste Chamber 1 Drain Error
24
Waste Chamber 2 Drain Error
24
Waste container is full
31
Water Leak Detected
23
Water Leak Sensor Error
24
Wrong reagent installed in UF-Fluorocell CR holder
44
Wrong reagent installed in UF-Fluorocell SF holder
44
X-bar Control Error
40
X-bar Control Error(internal QC)
40
U
☎ ☎ X
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Troubleshooting
[ErrorHelp] dialog box
When an error occurs, an alarm sounds and the [ErrorHelp] dialog box is displayed. If an error occurs while another dialog box is displayed, the error message will appear on the status area at the bottom of the screen. Close the displayed dialog box and touch the error message on the status area to display the [ErrorHelp] dialog box.
Error message list
Action message
[ErrorHelp] dialog box
Error message list
Displays the list of current errors and the time they occurred. If multiple errors exist, the errors will be listed in order of priority, with the highest at the top.
Action message
Displays the details and remedial actions for the error selected in the error message list.
[Reset Alarm]
Touch to stop the alarm that sounds when an error occurs.
[Step Guide]
Touch to display the dialog box where the procedure to clear the error selected in the error message list can be viewed. This button cannot be selected if there are no corresponding procedures.
[Err Recovery]/ [Rinse]/ [Shutdown]/ [Status Disp.]/ [Reagent Replace]/ [Recover]
Different buttons will appear depending on the type of error selected in the error message list. A button may not appear for some errors. [Err Recovery]: Touch to clear the error. [Rinse]: Touch to display the cleaning confirmation dialog box. (➤P.34 "Performing cleaning") [Shutdown]: Touch to display the shutdown confirmation dialog box. [Status Disp.]: Touch to display the [Status Disp.] dialog box. (➤P.65 "Chapter 2: 2.3 Checking the status of the instrument") [Reagent Replace]: Touch to display the [Reagent] dialog box. (➤P.82 "Chapter 2: 2.13.1 [Reagent] dialog box") [Recover]: Touch to start the error recovery process.
[Close]
Touch to close the dialog box.
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Note: • You can stop the alarm that sounds when an error occurs by touching any part of the touch panel. • Even after you touch [Close] and close the [ErrorHelp] dialog box, you can display the [ErrorHelp] dialog box by touching the error message on the status area.
1.3
Causes of errors and corrective actions
When an error occurs in the analyzer, refer to the causes and actions below and take appropriate action. Take the remedial action in order from case 1 if there are multiple possible causes.
Risk of infection • Be sure to wear adequate personal protective equipment, such as protective gloves, a protective mask, protective eyewear, and a lab coat when taking remedial actions for errors. After completion of work, wash hands with disinfectant. There is a risk of infection by pathogens or other contaminates. • Take appropriate care in handling samples. In the unlikely event that some infectious material gets in the eyes or an open wound, rinse with large amounts of water and seek immediate medical attention.
Errors related to pressure Abnormal Pressure Loss Probable cause
Actions
The instrument has failed.
The instrument requires repair or components require replacement. Contact your local Sysmex service representative. Remove the racks and sample tubes from the instrument, shut down the instrument, and then turn OFF the main power switch on the back of the instrument.
Sheath Pressure Error Probable cause
Actions
The sheath pressure adjustment is incorrect.
Touch [Status Disp.] in the [ErrorHelp] dialog box. Check the pressure in the [Status Disp.] dialog box and wait until the pressure falls within the specified range (0.06 to 0.14 MPa). Adjustment is required when it does not fall within the specified range. Contact your local Sysmex service representative.
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0.24MPa Pressure Error Probable cause
Actions
The 0.24 MPa pressure is incorrect.
Touch [Status Disp.] in the [ErrorHelp] dialog box. Adjust the pressure while checking the displayed [Status Disp.] dialog box. For details, see the following. (➤P.14 "Adjusting the 0.24 MPa pressure")
Adjusting the 0.24 MPa pressure 1 Touch [Status Disp.] in the [ErrorHelp] dialog box.
3 Adjust the pressure while checking the [0.24MPa pressure] value in the [Status Disp.] dialog box.
The [Status Disp.] dialog box appears.
Caution! • Always adjust the pressure by increasing it. If the pressure is too high, first lower it to a value that is below the adjustment range, and then slowly increase the pressure. If you adjust the pressure by decreasing it, there is a risk that the pressure cannot be adjusted correctly. • When you adjust the pressure, the value of other pressures may change slightly. Adjust so that all pressure values are in the correct range.
[Status Disp.] dialog box
2 While holding the 0.24 MPa regulator adjustment knob on the front of the pneumatic unit, loosen the fastening screw.
Turn the adjustment knob to adjust the pressure to 0.24 MPa (adjustment range: 0.228 to 0.252 MPa). The pressure increases when you turn the adjustment knob clockwise.
Loosen the screw by turning it in the direction of the arrow with a Phillips-head screwdriver.
Low High
Adjustment knob
Fastening screw
4 While holding the 0.24 MPa regulator adjustment knob, tighten the fastening screw. Tighten the screw by turning it in the direction opposite to step 2.
5 Touch [Close]. The dialog box closes and the error is cleared.
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0.05MPa Pressure Error Probable cause
Actions
The 0.05 MPa pressure is incorrect.
After the instrument stops, touch [Status Disp.] in the [ErrorHelp] dialog box. Adjust the pressure while checking the displayed [Status Disp.] dialog box. For details, see the following. (➤P.15 "Adjusting the 0.05 MPa pressure")
Adjusting the 0.05 MPa pressure 1 Touch [Status Disp.] in the [ErrorHelp] dialog box. The [Status Disp.] dialog box appears.
3 Remove the front lower cover. 1 Press the lock buttons (x2) on the front lower cover. The front lower cover unlocks.
Lock buttons
[Status Disp.] dialog box
Front lower cover
e.g. Sampler (SA-51)
2 Open the front upper cover. Open up the cover until it is locked. Front upper cover
Caution! When the front lower cover unlocks, it falls forward. When unlocking the front lower cover, press the upper part of the cover with your hand to keep the cover from falling.
2 Lift up the front lower cover and remove. Lift up the front lower cover so that the tabs (x2) on the analyzer separate from the notches on the bottom of the cover.
Tabs
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4 Unlock the 0.05 MPa pressure adjustment knob. Pull the adjustment knob in the direction of the arrow.
7 Attach the front lower cover. 1 Attach the front lower cover to the analyzer. First insert the tabs on the analyzer into the notches (x2) on the bottom of the front lower cover.
Adjustment knob
5 Adjust the pressure while checking the [0.05MPa Positive Press] value in the [Status Disp.] dialog box.
Caution! • Always adjust the pressure by increasing it. If the pressure is too high, first lower it to a value that is below the adjustment range, and then slowly increase the pressure. If you adjust the pressure by decreasing it, there is a risk that the pressure cannot be adjusted correctly. • When you adjust the pressure, the value of other pressures may change slightly. Adjust so that all pressure values are in the correct range.
Tabs
Then align the upper part of the front lower cover with the analyzer, and insert the tabs (x2) on the inner side of the lock buttons into the holes on the upper part of the analyzer.
2 Press in the lock buttons (x2) on the front lower cover. The front lower cover locks.
Caution! When locking the front lower cover, press the upper part of the cover with your hand.
Turn the adjustment knob to adjust the pressure to 0.05 MPa (adjustment range: 0.042 to 0.058 MPa). The pressure increases when you turn the adjustment knob clockwise.
Lock buttons Low
High
Front lower cover
8 Close the front upper cover. Gently press down on the cover to release the lock, and close the cover.
6 Push in the 0.05 MPa pressure adjustment knob to lock it. Take care that the adjustment knob does not turn.
9 Touch [Close]. The dialog box closes and the error is cleared.
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Vacuum Pressure Error Probable cause
Actions
Case 1: Fluid has collected in the trap chamber.
Check the volume of fluid in the trap chamber, and remove fluid if collected. For details, see the following. (➤P.17 "Checking the volume of fluid and removing fluid in the trap chamber")
Case 2: The vacuum adjustment is incorrect.
After the instrument stops, touch [Status Disp.] in the [ErrorHelp] dialog box. Adjust the pressure while checking the displayed [Status Disp.] dialog box. For details, see the following. (➤P.19 "Adjusting the vacuum")
Checking the volume of fluid and removing fluid in the trap chamber Risk of infection When disposing of fluid in the trap chamber, be sure to wear adequate personal protective equipment, such as protective gloves, a protective mask, protective eyewear, and a lab coat. After completion of work, wash hands with disinfectant. There is a risk of infection by pathogens or other contaminates.
Lock buttons Front lower cover
e.g. Sampler (SA-51)
Caution! If the trap chamber collects fluid even after taking the remedial action, the analyzer may have malfunctioned. Contact your local Sysmex service representative.
1 Open the front upper cover. Open up the cover until it is locked. Front upper cover
Caution! When the front lower cover unlocks, it falls forward. When unlocking the front lower cover, press the upper part of the cover with your hand to keep the cover from falling.
2 Lift up the front lower cover and remove. Lift up the front lower cover so that the tabs (x2) on the analyzer separate from the notches on the bottom of the cover.
2 Remove the front lower cover. 1 Press the lock buttons (x2) on the front
Tabs
lower cover. The front lower cover unlocks.
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3 Check if any fluid has collected in the trap chamber.
6 Attach the front lower cover. 1 Attach the front lower cover to the analyzer. First insert the tabs on the analyzer into the notches (x2) on the bottom of the front lower cover.
Trap chamber
If fluid has collected, go to step 4. If no fluid has collected, the following steps are not necessary. Attach the front lower cover. For details on attaching the front lower cover, see step 6.
4 Turn the trap chamber in the direction of the arrow to remove it, and dispose of the fluid collected in the chamber.
Tabs
Then align the upper part of the front lower cover with the analyzer, and insert the tabs (x2) on the inner side of the lock buttons into the holes on the upper part of the analyzer.
2 Press in the lock buttons (x2) on the front lower cover. The front lower cover locks.
Caution! When locking the front lower cover, press the upper part of the cover with your hand.
Float
Caution! • Be careful not to dispose of the float. • Hold the removed float in the same orientation and insert it straight into the trap chamber.
Lock buttons Front lower cover
5 Install the trap chamber by turning it in the direction opposite to that of step 4.
7 Close the front upper cover. Gently press down on the cover to release the lock, and close the cover.
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Troubleshooting
Adjusting the vacuum 1 Touch [Status Disp.] in the [ErrorHelp] dialog box. The [Status Disp.] dialog box appears.
Caution! When the front lower cover unlocks, it falls forward. When unlocking the front lower cover, press the upper part of the cover with your hand to keep the cover from falling.
2 Lift up the front lower cover and remove. Lift up the front lower cover so that the tabs (x2) on the analyzer separate from the notches on the bottom of the cover.
[Status Disp.] dialog box
2 Open the front upper cover. Open up the cover until it is locked. Front upper cover
Tabs
4 Loosen the fixing nut for the vacuum adjustment valve. Loosen the fixing nut by turning it in the direction of the arrow. Fixing nut
3 Remove the front lower cover. 1 Press the lock buttons (x2) on the front lower cover. The front lower cover unlocks.
Vacuum adjustment valve
Lock buttons Front lower cover
e.g. Sampler (SA-51)
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5 Adjust the pressure while checking the [0.05MPa vacuum] value in the [Status Disp.] dialog box.
2 Press in the lock buttons (x2) on the front lower cover. The front lower cover locks.
Caution!
Caution! • Always adjust the pressure by increasing it. If the pressure is too high, first lower it to a value that is below the adjustment range, and then slowly increase the pressure. If you adjust the pressure by decreasing it, there is a risk that the pressure cannot be adjusted correctly. • When you adjust the pressure, the value of other pressures may change slightly. Adjust so that all pressure values are in the correct range.
When locking the front lower cover, press the upper part of the cover with your hand.
Turn the adjustment knob to adjust the pressure to -0.053 MPa (adjustment range: -0.057 to -0.049 MPa). The pressure increases when you turn the adjustment knob clockwise.
Lock buttons Front lower cover
Adjustment knob
High
Low
8 Close the front upper cover. Gently press down on the cover to release the lock, and close the cover.
9 Touch [Close]. 6 Tighten the fixing nut for the vacuum
The dialog box closes and the error is cleared.
adjustment valve. Tighten the fixing nut by turning it in the direction opposite to step 4 while being careful that the adjustment knob does not turn.
7 Attach the front lower cover. 1 Attach the front lower cover to the analyzer. First insert the tabs on the analyzer into the notches (x2) on the bottom of the front lower cover.
Tabs
Then align the upper part of the front lower cover with the analyzer, and insert the tabs (x2) on the inner side of the lock buttons into the holes on the upper part of the analyzer.
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Troubleshooting
Errors related to temperature control Photomultiplier Sensor Chamber Temp High, Photomultiplier Sensor Chamber Temp Low Probable cause
Actions
The photomultiplier sensor chamber temperature is outside the specified range (33 to 37 °C).
Touch [Status Disp.] in the [ErrorHelp] dialog box. Check the temperature in the [Status Disp.] dialog box and wait until the temperature falls within the specified range. If the error is not cleared within 30 minutes after the error occurred, the instrument requires repair or components require replacement. Contact your local Sysmex service representative.
SF Reaction Chamber Temp High, SF Reaction Chamber Temp Low Probable cause
Actions
The MEMBRANE ch reaction chamber temperature is outside the specified range (36 to 43 °C).
Touch [Status Disp.] in the [ErrorHelp] dialog box. Check the temperature in the [Status Disp.] dialog box and wait until the temperature falls within the specified range. If the error is not cleared within 30 minutes after the error occurred, the instrument requires repair or components require replacement. Contact your local Sysmex service representative.
CR Reaction Chamber Temp High, CR Reaction Chamber Temp Low Probable cause
Actions
The NUCLEUS ch reaction chamber temperature is outside the specified range (36 to 43 °C).
Touch [Status Disp.] in the [ErrorHelp] dialog box. Check the temperature in the [Status Disp.] dialog box and wait until the temperature falls within the specified range. If the error is not cleared within 30 minutes after the error occurred, the instrument requires repair or components require replacement. Contact your local Sysmex service representative.
Sheath Temperature Chamber Temp High, Sheath Temperature Chamber Temp Low Probable cause
Actions
The sheath temperature chamber temperature is outside the specified range (33 to 50 °C).
Touch [Status Disp.] in the [ErrorHelp] dialog box. Check the temperature in the [Status Disp.] dialog box and wait until the temperature falls within the specified range. If the error is not cleared within 30 minutes after the error occurred, the instrument requires repair or components require replacement. Contact your local Sysmex service representative.
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Troubleshooting
SF Preheating Unit Temp High, SF Preheating Unit Temp Low Probable cause
Actions
The SF preheating unit temperature is outside the specified range (38 to 50 °C).
Touch [Status Disp.] in the [ErrorHelp] dialog box. Check the temperature in the [Status Disp.] dialog box and wait until the temperature falls within the specified range. If the error is not cleared within 30 minutes after the error occurred, the instrument requires repair or components require replacement. Contact your local Sysmex service representative.
CR Preheating Unit Temp High, CR Preheating Unit Temp Low Probable cause
Actions
The CR preheating unit temperature is outside the specified range (38 to 50 °C).
Touch [Status Disp.] in the [ErrorHelp] dialog box. Check the temperature in the [Status Disp.] dialog box and wait until the temperature falls within the specified range. If the error is not cleared within 30 minutes after the error occurred, the instrument requires repair or components require replacement. Contact your local Sysmex service representative.
Conductivity Analysis Chamber Temp High, Conductivity Analysis Chamber Temp Low Probable cause
Actions
The conductivity analysis chamber temperature is outside the specified range (38 to 42 °C).
Touch [Status Disp.] in the [ErrorHelp] dialog box. Check the temperature in the [Status Disp.] dialog box and wait until the temperature falls within the specified range. If the error is not cleared within 30 minutes after the error occurred, the instrument requires repair or components require replacement. Contact your local Sysmex service representative.
Detector Base Chamber Temp High, Detector Base Chamber Temp Low Probable cause
Actions
The detector base chamber temperature is outside the specified range (33 to 37 °C).
Touch [Status Disp.] in the [ErrorHelp] dialog box. Check the temperature in the [Status Disp.] dialog box and wait until the temperature falls within the specified range. If the error is not cleared within 30 minutes after the error occurred, the instrument requires repair or components require replacement. Contact your local Sysmex service representative.
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Troubleshooting
Sheath Fluid Temp High, Sheath Fluid Temp Low Probable cause
Actions
The sheath fluid temperature is not within the specified temperature range (10 to 37 °C).
Replace with sheath fluid that is within the specified temperature range. For details, see the following. (➤P.25 "Replacing diluent and sheath fluid")
Room temperature is high, Room temperature is low Probable cause
Actions
The room temperature is outside the specified range (10 to 30 °C).
Touch [Status Disp.] in the [ErrorHelp] dialog box. Adjust the room temperature while checking the displayed [Status Disp.] dialog box.
Temperature Stabilizing Error Probable cause
Actions
The temperature does not stabilize within 20 minutes after the instrument is started.
The instrument requires repair or components require replacement. Contact your local Sysmex service representative. Remove the racks and sample tubes from the instrument, shut down the instrument, and then turn OFF the main power switch on the back of the instrument.
Sheath Temperature Chamber Thermistor Error, Guide Liquid Chamber Thermistor Error, SF Reaction Chamber Thermistor Error, SF Preheating Unit Thermistor Error, CR Reaction Chamber Thermistor Error, CR Preheating Unit Thermistor Error, Conductivity Analysis Chamber Thermistor Error, Photomultiplier Sensor Chamber Thermistor Error, Detector Base Chamber Thermistor Error, Room Temperature Thermistor Error Probable cause
Actions
The thermistor has failed or broken.
The instrument requires repair or components require replacement. Contact your local Sysmex service representative. Remove the racks and sample tubes from the instrument, shut down the instrument, and then turn OFF the main power switch on the back of the instrument.
Errors related to hydraulic system Water Leak Detected Probable cause
Actions
There is a water leak inside the instrument.
The instrument requires repair or components require replacement. Contact your local Sysmex service representative. Remove the racks and sample tubes from the instrument, shut down the instrument, and then turn OFF the main power switch on the back of the instrument.
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Water Leak Sensor Error Probable cause
Actions
The water leak sensor has failed or broken.
The instrument requires repair or components require replacement. Contact your local Sysmex service representative. Remove the racks and sample tubes from the instrument, shut down the instrument, and then turn OFF the main power switch on the back of the instrument.
Waste Chamber 1 Drain Error, Waste Chamber 2 Drain Error Probable cause
Actions
The drain tube is bent or clogged.
Straighten the drain tube and remove the clog in the tube, and touch [Recover] in the [ErrorHelp] dialog box to perform the recovery process.
UF-CELLSHEATH Aspiration Error(FCM), UF-CELLSHEATH Aspiration Error(Rinse) Probable cause
Actions
Case 1: The reagent has run out.
Replace the reagent with a new one. For details, see the following. (➤P.25 "Replacing diluent and sheath fluid")
Case 2: The nipple connected to the reagent container is loose or the tube is broken.
Check for loose nipples and tubes, and reconnect them if loose. For details on the position of nipples, see "General Information". (➤General Information "Chapter 3: 3.1 Analyzer") When a problem such as tube breakage occurs, the instrument requires repair or components require replacement. Contact your local Sysmex service representative.
Case 3: Float switch is broken or there is an abnormality in the hydraulic system.
The instrument requires repair or components require replacement. Contact your local Sysmex service representative. Remove the racks and sample tubes from the instrument, shut down the instrument, and then turn OFF the main power switch on the back of the instrument.
Air Bubble in UF-Fluorocell SF Line, Air Bubble in UF-Fluorocell CR Line Probable cause
Actions
Aspiration of the reagent has failed.
Touch [Recover] in the [ErrorHelp] dialog box. Replace the reagent with a new one if the error is not cleared. For details, see the following. (➤P.25 "Replacing diluent and sheath fluid")
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Errors related to reagent and rinse water Out of UF-CELLSHEATH, Out of UF-CELLPACK CR, Out of UF-CELLPACK SF Probable cause
Actions
Case 1: The reagent has run out.
Replace the reagent with a new one. For details, see the following. (➤P.25 "Replacing diluent and sheath fluid")
Case 2: There is a foreign object pressing on the tubing of the spout set connected to the reagent container. The tubing is broken or disconnected.
Remove the object that is pressing on the tubing, and straighten the tubing. Reconnect the tubing if it is disconnected.
UF-CELLSHEATH has expired, UF-CELLPACK CR has expired, UF-CELLPACK SF has expired Probable cause
Actions
The expiration date or shelf life after first opening of the reagent has passed.
Replace the reagent with a new one. For details, see the following. (➤P.25 "Replacing diluent and sheath fluid")
Replacing diluent and sheath fluid 2 Touch [Reagent Replace] in the Caution! • Do not add new reagent to existing reagent. • Place the reagent at a level no more than 1 meter above or below the bottom of the analyzer. Do not put reagents on top of the instrument. • The new reagent must be left undisturbed for over 24 hours at 10 to 30 °C before using. • Take care not to spill reagent. In case of spillage, wipe immediately with a damp cloth. Otherwise, remained reagent on the surface of instrument may cause staining or deterioration. • Read the indication of the reagent container or package box, Safety Data Sheet, and "General Information" before handling reagents. (➤General Information "Chapter 7 Reagents")
1 Prepare new reagent. Check that the reagent has not expired.
[ErrorHelp] dialog box. The [Reagent] dialog box appears. (➤P.82 "Chapter 2: 2.13.1
[Reagent] dialog box")
When [Close] in the [ErrorHelp] dialog box is touched Touch the [Reagents Replacement] icon on the [Menu] screen to display the [Reagent] dialog box.
3 Enter the Reagent Code of the new reagent. Enter by barcode reading Read the Reagent Code (barcode) of the new reagent with hand-held barcode reader. The Reagent Code is located on the outer box of sheath fluid and on the container of diluent. Reagent Code is as shown below.
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When the Reagent Code is entered, the reagent in the [Reagent] dialog box displays [Received]. The expiration date and the lot number of the new reagent also appear.
5 Remove the cap from the old reagent container. For UF-CELLSHEATH, turn lower part of the cap in the direction of the arrow to remove it.
Note: When entering the Reagent Code manually, touch the reagent to be replaced in the [Reagent] dialog box. The following dialog box appears. For UF-CELLSHEATH For UF-CELLPACK CR/UF-CELLPACK SF, turn the cap in the direction of the arrow to remove it.
Enter the [Reagent Code], and touch [Register]. If the dialog box for entering a lot number appears, follow the instructions in the dialog box to register the reagent information.
4 Remove the cap from the new reagent container.
For UF-CELLPACK CR/UF-CELLPACK SF
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6 Pull out the float switch/spout kit straight up.
Troubleshooting
8 Touch [Replace] in the [Reagent] dialog box. The progress dialog box appears, and reagent replacement starts. The progress dialog box closes when reagent replacement is complete, and the information displayed in the [Reagent] dialog box is updated. The time guidelines for reagent replacement are as shown below.
Reagent name UF-CELLSHEATH For UF-CELLSHEATH
Time Approx. 1 minute
UF-CELLPACK CR
Approx. 2 minutes
UF-CELLPACK SF
Approx. 2 minutes
For UF-CELLPACK CR/UF-CELLPACK SF
Caution! Do not touch the aspiration nozzle of the float switch/ spout kit. Keep dust, dirt, bacteria, and other contaminants away from the aspiration nozzle and the inside of the container.
7 Insert the float switch/spout kit straight into the new reagent container and close the cap. Close the cap by turning it in the opposite direction of step 5.
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Out of UF-Fluorocell CR, Out of UF-Fluorocell SF Probable cause
Actions
The reagent has run out.
Replace the reagent with a new one. For details, see the following. (➤P.28 "Replacing dye")
UF-Fluorocell CR has expired, UF-Fluorocell SF has expired Probable cause
Actions
The expiration date or shelf life after first opening of the reagent has passed.
Replace the reagent with a new one. For details, see the following. (➤P.28 "Replacing dye")
Cannot recognize UF-Fluorocell SF information, Cannot recognize UF-Fluorocell CR information Probable cause
Actions
The RFID tag on the reagent cartridge is damaged.
Replace the reagent with a new one. For details, see the following. (➤P.28 "Replacing dye")
Replacing dye 2 Touch [Reagent Replace] in the Caution! • Be sure to wear adequate personal protective equipment, such as protective gloves, a protective mask, protective eyewear, and a lab coat when replacing the reagent. • Do not add new reagent to existing reagent. • Read the labels on the reagent containers and/or boxes, the Instructions for Use and the Safety Data Sheet, and "General Information" before handling reagents. (➤General Information "Chapter 7 Reagents")
[ErrorHelp] dialog box. The [Reagent] dialog box appears. (➤P.82 "Chapter 2: 2.13.1
[Reagent] dialog box")
When [Close] in the [ErrorHelp] dialog box is touched Touch the [Reagents Replacement] icon on the [Menu] screen to display the [Reagent] dialog box.
3 Open the front upper cover. Open up the cover until it is locked.
Information
Front upper cover
Do not replace dye while the instrument is in operation. If you replace dye during operation, the RFID tag will be damaged, and the information will be unable to be read properly.
1 Prepare a new reagent cartridge. Check that the reagent has not expired.
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4 Pull up the cover from the dye that is to
Troubleshooting
6 Attach the new reagent cartridge into
be replaced.
the holder.
Pull up the cover until it clicks.
Analyzer beeps.
e.g. UF-Fluorocell CR
The position to attach dye is shown below.
Caution! SF
CR
5 Remove the old reagent cartridge from its holder.
• If you attach a wrong reagent, an error occurs and an alarm sounds. Check the error and attach the correct reagent. • Take care not to spill reagent. In case of spillage, wipe immediately with a damp cloth. Otherwise, the coated surface of the instrument could be stained or deteriorated.
7 Pull down the cover on the dye. Reagent replacement is executed. The expiration date and lot number of the new reagent appears in the [Reagent] dialog box.
8 Close the front upper cover. Reagent cartridge
Gently press down on the cover to release the lock, and close the cover.
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No Rinse Water Probable cause
Actions
The rinse water has run out.
Replenish the rinse water. For details, see the following. (➤P.30 "Replenishing rinse water")
Replenishing rinse water 3 Pull out the spout kit straight up. Caution! Do not use anything OTHER than purified water for the rinse water. Risk of instrument failure and inaccurate analysis results.
1 Touch [Reagent Replace] in the [ErrorHelp] dialog box. The [Reagent] dialog box appears. (➤P.82 "Chapter 2: 2.13.1
[Reagent] dialog box")
When [Close] in the [ErrorHelp] dialog box is touched Touch the [Reagents Replacement] icon on the [Menu] screen to display the [Reagent] dialog box.
2 Remove the cap from the rinse water
Caution! Do not touch the aspiration nozzle of the spout kit. Keep dust, dirt, bacteria, and other contaminants away from the aspiration nozzle and the inside of the container.
bottle. Turn the cap in the direction of the arrow to remove it.
4 Replenish the rinse water. Fill the rinse water bottle with rinse water until it is full.
5 Insert the spout kit straight into the rinse bottle.
6 Close the cap of the rinse bottle. Close the cap by turning it in the opposite direction of step 2.
7 Touch [Replace] in the [Reagent] dialog box. The progress dialog box appears and replenishment of rinse water starts. Replenishment takes approximately 2 minutes. The progress dialog box closes when replenishment is complete, and the information displayed in the [Reagent] dialog box is updated.
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Errors related to waste fluid Waste container is full Probable cause
Actions
The waste tank is full.
Dispose of the waste fluid in the tank. For details, see the following. (➤P.31 "Disposing of waste fluid in the waste tank")
Disposing of waste fluid in the waste tank Risk of infection
2 Remove the cap from the waste tank. Turn the cap in the direction of the arrow to remove it.
• Be sure to wear adequate personal protective equipment, such as protective gloves, a protective mask, protective eyewear, and a lab coat when disposing of waste fluid. After completion of work, wash hands with disinfectant. There is a risk of infection by pathogens or other contaminates. • Waste fluid and other potentially infected items must be separated from general waste and treated according to applicable local and national regulations.
1 Make sure the instrument is in the ready state for sampler analysis or STAT analysis. If the instrument is not in the ready state, wait until the instrument status becomes [Sampler Ready], [Sampler Int. Ready], or [Stat Ready].
Risk of infection Do not dispose of waste fluid while the instrument mechanisms are in motion, such as during analysis. Waste fluid could spray out of the tube and onto your skin or clothes, causing a risk of infection by pathogens or other contaminates.
3 Dispose of the waste fluid. 4 Rinse the waste tank. 5 Disinfect with alcohol the inside of the waste tank, the cap, and any parts that were in contact with waste fluid.
6 Close the cap of the waste tank. Caution! Place the waste tank below the bottom of the instrument.
7 Touch [Recover] in the [ErrorHelp] dialog box. The error is cleared.
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Errors related to mixing/aspiration/analysis Mixing/Aspiration Unit Up/Down Mechanism Motor Error, Mixing/Aspiration Unit Forward/Back Mechanism Motor Error, Mixing Motor(CR) Speed Error, Mixing Motor(SF) Speed Error, Dispensing Unit Up/Down Mechanism Motor Error, Dispensing Unit Left/Right Mechanism Motor Error Probable cause
Actions
The motor did not work properly.
Touch [Recover] in the [ErrorHelp] dialog box. The instrument requires repair or components require replacement if the error is not cleared. Contact your local Sysmex service representative.
Urine Pump Error, Charging Pump Error, Dispensing Pump Error Probable cause
Actions
The pump did not work properly.
Touch [Recover] in the [ErrorHelp] dialog box. The instrument requires repair or components require replacement if the error is not cleared. Contact your local Sysmex service representative.
Sheath Syringe Error Probable cause
Actions
The syringe did not work properly.
Touch [Recover] in the [ErrorHelp] dialog box. The instrument requires repair or components require replacement if the error is not cleared. Contact your local Sysmex service representative.
Mixing/Aspiration Unit Probe Crash, Dispensing Unit Probe Crash Probable cause
Actions
The probe did not work properly.
Touch [Recover] in the [ErrorHelp] dialog box. The instrument requires repair or components require replacement if the error is not cleared. Contact your local Sysmex service representative.
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Errors related to laser Laser Output Error Probable cause
Actions
The laser diode has deteriorated.
The instrument requires repair or components require replacement. Contact your local Sysmex service representative. Remove the racks and sample tubes from the instrument, shut down the instrument, and then turn OFF the main power switch on the back of the instrument.
Laser Radiation Error Probable cause
Actions
It is time to replace the laser diode.
The instrument requires repair or components require replacement. Contact your local Sysmex service representative. Remove the racks and sample tubes from the instrument, shut down the instrument, and then turn OFF the main power switch on the back of the instrument.
The service life of the laser has nearly expired. Probable cause
Actions
Case 1: Abnormal oscillation has occurred temporarily.
Touch [Err Recovery] in the [ErrorHelp] dialog box.
Case 2: It is nearly time to replace the laser diode.
Components require replacement. Contact your local Sysmex service representative. Touch [Err Recovery] in the [ErrorHelp] dialog box.
Errors related to fan Substrate Cooling Fan Error, Sampler Unit Fan Error, Power Unit Fan Error, Back Exhaust Fan Error Probable cause
Actions
The fan has failed.
The instrument requires repair or components require replacement. Contact your local Sysmex service representative.
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Errors related to sample aspiration Short Sample(Mixing), Short Sample(Dispensing)* Probable cause
Actions
Case 1: Sample volume is insufficient.
Touch [Err Recovery] in the [ErrorHelp] dialog box, and then place a sufficient volume of the sample.
Case 2: The tubing of the sample aspiration line is dirty or clogged.
Perform instrument cleaning. For details, see the following. (➤P.34 "Performing cleaning")
Case 3: Sample filter is clogged.
Rinse or replace the sample filter. For details, see the following. (➤P.35 "Rinsing/replacing the sample filter")
* The cause of a [Short Sample(Dispensing)] error can be viewed from the [Error Log] screen (➤P.103 "Chapter 3: 3.2.2 [Error Log] screen"). The cause of the error is displayed in the [Error Parameter 1] column as shown below: • [1]: Sample volume is insufficient. • [2]: Sample filter is clogged.
Performing cleaning 1 Make sure the instrument is in the ready state for sampler analysis or STAT analysis. If the instrument is not in the ready state, wait until the instrument status becomes [Sampler Ready], [Sampler Int. Ready], or [Stat Ready].
2 Touch [Rinse] in the [ErrorHelp] dialog box. The confirmation dialog box appears.
3 Touch [Yes]. The dialog box closes and cleaning starts. Progress is shown in a progress bar on the status area at the bottom of the screen. Cleaning takes about 10 minutes. Wait until it is complete.
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Rinsing/replacing the sample filter 6 Open the front upper cover. Risk of infection Be sure to wear adequate personal protective equipment, such as protective gloves, a protective mask, protective eyewear, and a lab coat when rinsing or replacing the sample filter. After completion of work, wash hands with disinfectant. There is a risk of infection by pathogens or other contaminates.
Open up the cover until it is locked. Front upper cover
Warning! Be sure to shut down the instrument before rinsing or replacing the sample filter. This is necessary to avoid the risk of electric shock.
1 Make sure the instrument is in the ready state for sampler analysis or STAT analysis.
7 Remove the front lower cover. 1 Press the lock buttons (x2) on the front lower cover. The front lower cover unlocks.
If the instrument is not in the ready state, wait until the instrument status becomes [Sampler Ready], [Sampler Int. Ready], or [Stat Ready].
2 Touch the [Maintenance] icon on the [Menu] screen. The [Maintenance Menu] screen appears. (➤P.64 "Chapter 2: 2.2 screen")
[Maintenance Menu]
3 Touch the [Sample filter] icon.
Lock buttons Front lower cover
e.g. Sampler (SA-51)
The confirmation dialog box appears.
4 Touch [Execute]. The sample filter starts moving, and the progress dialog box appears. When the sample filter has moved to the replacement position, the dialog box closes, and the power to the instrument automatically turns OFF.
Caution! When the front lower cover unlocks, it falls forward. When unlocking the front lower cover, press the upper part of the cover with your hand to keep the cover from falling.
5 Wait 1 minute to allow the pressure to fall inside the instrument.
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2 Lift up the front lower cover and remove. Lift up the front lower cover so that the tabs (x2) on the analyzer separate from the notches on the bottom of the cover.
9 Remove the tube adapters from the top and bottom of the sample filter. Turn the top and bottom tube adapters in the direction of the arrow to remove them. Tube adapter (top)
Tabs
8 Remove the sample filter case from the
Tube adapter (bottom)
holder.
Warning! Have a dry cloth ready in case fluid leaks from the tube when removing the sample filter. The risk of electrical shock is high when your hands are wet.
Caution! • Do not forcibly pull the tube when removing the tube adapter. • Do not bend the tube. This is necessary to avoid the damage to the tube.
10 Remove the cap from the sample filter Holder
case. Turn the cap in the direction of the arrow to remove it. Cap
Sample filter case
Caution! Be careful not to drop the filter inside the case when removing the cap from the sample filter case.
Filter
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11 Remove the filter from the sample filter
Troubleshooting
13 Install the tube adapters onto the top
case, and rinse it with purified water.
and bottom of the sample filter.
After rinsing, use a dry cloth to dry the filter gently. Check whether or not there is clogging in the hole, and rinse the filter again if there is clogging. If the clogging cannot be eliminated, replace the filter.
Install the top and bottom tube adapters by turning them in the opposite direction of step 9 with the cap up as shown. Tube adapter (top) Cap
Filter
Tube adapter (bottom)
14 Install the sample filter case into the
Caution! If there is a clog in the sample filter, this may have affected analysis count values obtained before the filter was rinsed or replaced. Check if there are any abnormalities in the analysis result data.
holder.
Sample filter case
12 Set the rinsed or new filter in the sample filter case, and attach the cap. Attach the cap by turning it in the opposite direction of step 10.
Holder
Close the cap firmly. Cap
Filter
15 Attach the front lower cover. 1 Attach the front lower cover to the analyzer. First insert the tabs on the analyzer into the notches (x2) on the bottom of the front lower cover.
Sample filter case
Tabs
Then align the upper part of the front lower cover with the analyzer, and insert the tabs (x2) on the inner side of the lock buttons into the holes on the upper part of the analyzer.
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2 Press in the lock buttons (x2) on the
● When the sampler (CV-11) is connected
front lower cover.
Press the startup switch on the sampler (CV-11).
The front lower cover locks.
Caution! When locking the front lower cover, press the upper part of the cover with your hand.
Startup switch
Log on to the instrument after startup.
Lock buttons
18 Perform QC analysis. For details on QC analysis, see "Basic Operation".
Front lower cover
(➤Basic Operation "Chapter 2: 2.6 Performing QC analysis")
16 Close the front upper cover.
Check the following points, and reassemble the sample filter if there is a problem. • A short sample error does not occur • There is no fluid leakage from the filter • There is no abnormality with the QC analysis results
Gently press down on the cover to release the lock, and close the cover.
17 Turning ON the power to the instrument. ● When the sampler (SA-51) is connected Press the power switch on the sampler (SA-51).
1 1
Power switch
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Errors related to sample holder Tube remains in tube holder Probable cause
Actions
The tube remains in the STAT sample tube holder.
Remove the sample tube from the STAT sample tube holder, and then touch [Err Recovery] in the [ErrorHelp] dialog box.
Errors related to barcode Failed to read sample number Probable cause
Actions
• The barcode label is dirty. • The print quality of the barcode label is poor. • The position and orientation of the barcode label is off.
Check the position, orientation, and dirt of the barcode label, and then touch [Err Recovery] in the [ErrorHelp] dialog box.
Barcode Reader Communication Error Probable cause
Actions
There was a communication error with the barcode reader.
The instrument requires repair or components require replacement. Contact your local Sysmex service representative. Remove the racks and sample tubes from the instrument, shut down the instrument, turn OFF and then ON the main power switch on the back of the instrument to restart the instrument.
Barcode Reader Position Error Probable cause
Actions
The barcode reader did not work properly.
Remove the racks and sample tubes from the instrument, and then touch [Recover] in the [ErrorHelp] dialog box.
Positive ID Check Error Probable cause
Actions
The 1st barcode reading result did not match the 2nd barcode reading result.
Check the instrument for foreign objects, and touch [Err Recovery] in the [ErrorHelp] dialog box.
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Errors related to system Conveyor Communication Error Probable cause
Actions
There was a communication error with the sampler (CV-11).
Check the connection with the sampler (CV-11). If an error occurs even though there is no problem in the connection, contact your local Sysmex service representative.
RFID Communication Error Probable cause
Actions
There was a communication error with the RFID device.
The instrument requires repair or components require replacement. Contact your local Sysmex service representative. Remove the racks and sample tubes from the instrument, shut down the instrument, turn OFF and then ON the main power switch on the back of the instrument to restart the instrument.
Host computer Communication Error Probable cause
Actions
There was a communication error with the host computer.
Check the connection with the host computer. If an error occurs even though there is no problem in the connection, contact your local Sysmex service representative.
Errors related to quality control L-J Control Error, L-J Control Error(internal QC), X-bar Control Error, X-bar Control Error(internal QC) Probable cause
Actions
An error (a value outside the QC limits) has occurred in quality control.
Touch [Err Recovery] in the [ErrorHelp] dialog box, and then check the result of the QC analysis on the [QC Chart] screen. For details on checking results of the QC analysis, see "Basic Operation". (➤Basic Operation "Chapter 2: 2.7 Checking QC analysis results")
Control Material Expired Error Probable cause
Actions
The control material has expired.
Touch [Err Recovery] in the [ErrorHelp] dialog box, and perform QC analysis using a new control material within the expiration date. For details on performing the QC analysis, see "Basic Operation". (➤Basic Operation "Chapter 2 Performing Quality Control")
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Errors related to analysis Background Error(Urine), Background Error(Body Fluid) Probable cause
Actions
Air bubbles have formed in the detector. The detector is dirty or clogged.
Perform instrument cleaning. For details, see the following. (➤P.42 "Performing cleaning")
BACT High Probable cause
Actions
Analyzed samples with high values that may affect the next analysis results.
Perform instrument cleaning. For details, see the following. (➤P.42 "Performing cleaning")
Sampling Error Probable cause
Actions
The concentration of the sample is inconsistent.
Touch [Err Recovery] in the [ErrorHelp] dialog box.
Abnormaly high RBC Probable cause
Actions
Analyzed samples with high values that may affect the analysis results.
Touch [Err Recovery] in the [ErrorHelp] dialog box. Perform instrument cleaning if the anti-carryover function (auto rinse) is disabled. For details, see the following. (➤P.42 "Performing cleaning")
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Performing cleaning 1 Make sure the instrument is in the ready state for sampler analysis or STAT analysis. If the instrument is not in the ready state, wait until the instrument status becomes [Sampler Ready], [Sampler Int. Ready], or [Stat Ready].
2 Touch [Rinse] in the [ErrorHelp] dialog box. The confirmation dialog box appears.
3 Touch [Yes]. The dialog box closes and cleaning starts. Progress is shown in a progress bar on the status area at the bottom of the screen. Cleaning takes about 10 minutes. Wait until it is complete.
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Errors related to covers Front cover is open Probable cause
Actions
Front lower cover is removed.
Attach the cover, and then touch [Recover] in the [ErrorHelp] dialog box.
Detector cover is open Probable cause
Actions
Detector cover is open.
Touch [Recover] in the [ErrorHelp] dialog box.
UF-Fluorocell CR reagent cover is open, UF-Fluorocell SF reagent cover is open Probable cause
Actions
Dye cover is open.
Close the cover.
Errors related to replacement parts Replace the sheath syringe, Replace the Seal of the urine pump, Replace the Seal of the charging pump, Replace the dispensing pump Probable cause
Actions
It is time to replace the syringe/pump/pump seal.
The instrument requires repair or components require replacement. Contact your local Sysmex service representative.
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Errors related to dye holder Wrong reagent installed in UF-Fluorocell CR holder, Wrong reagent installed in UF-Fluorocell SF holder Probable cause
Actions
• Placement positions of UF-Fluorocell CR and UF-Fluorocell SF are wrong. • The wrong type of cartridge is attached.
Remove the reagent that was attached in the wrong position, and then attach new reagent in the correct position. For details, see the following. (➤P.45 "Replacing dye") If you closed the cover on the dye with the wrong reagent attached, the wrong reagent may have mixed in. Discard without using.
UF-Fluorocell CR is not installed, UF-Fluorocell SF is not installed Probable cause
Actions
Dye is not attached.
Attach the reagent. For details, see the following. (➤P.45 "Replacing dye")
UF-Fluorocell CR has already been used, UF-Fluorocell SF has already been used Probable cause
Actions
A used dye is attached.
Replace the reagent with a new one. For details, see the following. (➤P.45 "Replacing dye")
UF-Fluorocell CR RFID tag error, UF-Fluorocell SF RFID tag error Probable cause
Actions
Cannot write any data to the RFID tag of the reagent cartridge.
Replace with new reagent (with normal RFID). For details, see the following. (➤P.45 "Replacing dye")
UF-Fluorocell SF replaced during operation, UF-Fluorocell CR replaced during operation Probable cause
Actions
Dye has been replaced while the instrument is in operation.
Remove the reagent after the instrument is completely stopped, and set the reagent again. For details, see the following. (➤P.45 "Replacing dye")
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Replacing dye 4 Pull up the cover from the dye that is to Caution! • Be sure to wear adequate personal protective equipment, such as protective gloves, a protective mask, protective eyewear, and a lab coat when replacing the reagent. • Do not add new reagent to existing reagent. • Read the labels on the reagent containers and/or boxes, the Instructions for Use and the Safety Data Sheet, and "General Information" before handling reagents. (➤General Information "Chapter 7 Reagents")
be replaced. Pull up the cover until it clicks.
e.g. UF-Fluorocell CR
Information Do not replace dye while the instrument is in operation. If you replace dye during operation, the RFID tag will be damaged, and the information will be unable to be read properly.
The position to attach dye is shown below.
SF
CR
1 Prepare a new reagent cartridge. Check that the reagent has not expired.
5 Remove the old reagent cartridge from its holder.
2 Touch the [Reagents Replacement] icon on the [Menu] screen.
Reagent cartridge
The [Reagent] dialog box appears. (➤P.82 "Chapter 2: 2.13.1 [Reagent] dialog box")
3 Open the front upper cover. Open up the cover until it is locked. Front upper cover
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6 Attach the new reagent cartridge into the holder. Analyzer beeps.
Caution! • If you attach a wrong reagent, an error occurs and an alarm sounds. Check the error and attach the correct reagent. • Take care not to spill reagent. In case of spillage, wipe immediately with a damp cloth. Otherwise, the coated surface of the instrument could be stained or deteriorated.
7 Pull down the cover on the dye. Reagent replacement is executed. The expiration date and lot number of the new reagent appears in the [Reagent] dialog box.
8 Close the front upper cover. Gently press down on the cover to release the lock, and close the cover.
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Errors related to sampler analysis operation Rack Feed-in Home Position Error, Rack Feed-in Error Probable cause
Actions
Case 1: There is a foreign object or dirt in the right sampler pool.
Remove the foreign object and clean the right sampler pool, and then touch [Recover] in the [ErrorHelp] dialog box.
Case 2: The rack is not set correctly.
Set the rack in the correct position, and then touch [Recover] in the [ErrorHelp] dialog box.
Case 3: The rack sensor is dirty or broken.
The instrument requires repair or components require replacement. Contact your local Sysmex service representative. Remove the racks and sample tubes from the instrument, shut down the instrument, and then turn OFF the main power switch on the back of the instrument.
Rack Move Mechanism Initialization Error(Front Belt), Rack Move Mechanism Initialization Error(Back Belt), Rack Move Error(Front Belt), Rack Move Error(Back Belt) Probable cause
Actions
There is a foreign object on the sampler's analysis line. The rack is not set correctly.
Remove the racks and sample tubes from the instrument, and then touch [Recover] in the [ErrorHelp] dialog box.
Rack Feed-out Position Error, Rack Feed-out Error Probable cause
Actions
Case 1: There is a foreign object near the rack feed-out lever.
Remove the foreign object away from the lever, and then touch [Recover] in the [ErrorHelp] dialog box.
Case 2: There is a foreign object or dirt in the left sampler pool.
Remove the foreign object and clean the left sample pool, and then touch [Recover] in the [ErrorHelp] dialog box.
Case 3: Rack feed-out operation is obstructed.
Set the rack in the correct position, and then touch [Recover] in the [ErrorHelp] dialog box.
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Rack is full Probable cause
Actions
Case 1: The left sampler pool is full with racks.
Remove racks from the left sampler pool.
Case 2: There is a foreign object or dirt in the left sampler pool.
Remove the foreign object and clean the left sample pool.
Case 3: The rack sensor is dirty or broken.
The instrument requires repair or components require replacement. Contact your local Sysmex service representative. Remove the racks and sample tubes from the instrument, shut down the instrument, and then turn OFF the main power switch on the back of the instrument.
Rack is not placed Probable cause
Actions
Racks are not placed in the right sampler pool.
Touch [Err Recovery] in the [ErrorHelp] dialog box, and then place racks.
Feed-in Table Stopper Position Error 1, Feed-in Table Stopper Position Error 2 Probable cause
Actions
There is a foreign object near the feed-in table stopper.
Remove the foreign object away from the feed-in table stopper. Remove the racks and sample tubes from the instrument, and then touch [Recover] in the [ErrorHelp] dialog box.
Sampler Belt Error Probable cause
Actions
Rack on the analysis line prior to analysis.
Remove the rack on the analysis line, and then touch [Recover] in the [ErrorHelp] dialog box.
Rack Exclusion Error Probable cause
Actions
Rack exclusion is obstructed.
Touch [Err Recovery] in the [ErrorHelp] dialog box, and then remove foreign objects from the analysis line. Remove the rack on the analysis line, and then press the start switch of the sampler (CV-11).
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Sampler driver error Probable cause
Actions
The sampler (SA-51) driver version is not compatible with the application version of the instrument.
The sampler driver must be reinstalled. Contact your local Sysmex service representative. Remove the racks and sample tubes from the instrument, shut down the instrument, and then turn OFF the main power switch on the back of the instrument.
Other Power was turned OFF without executing shutdown Probable cause
Actions
The power was turned OFF without shutting down the instrument.
Touch [Err Recovery] in the [ErrorHelp] dialog box. Check if there are any abnormalities in the analysis result data stored in the instrument.
Execute the shutdown Probable cause
Actions
24 hours has elapsed since the instrument started.
Touch [Shutdown] in the [ErrorHelp] dialog box. Touch [Yes] in the displayed confirmation dialog box to shut down the instrument. To continue using the instrument, restart it. For details, see "Basic Operation". (➤Basic Operation "Chapter 1: 1.4 Restarting")
Caution! • If the following dialog box appears, touch [Shutdown] to shutdown the instrument. When shutdown is finished, contact your local Sysmex service representative. • Do not turn ON the instrument until Sysmex technical representative arrives. When the sampler (CV-11) is connected, do not press the startup switch on the sampler (CV-11).
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Troubleshooting
Remedial actions against U-RDU failure
Even when it is impossible to deliver reagent to the instrument due to U-RDU failure, you can still use the instrument by switching the reagent tube connection to directly deliver the reagent to the instrument. Refer to the procedure below and take appropriate action. Note that this section describes the procedure for when a maximum of 6 instruments and a maximum of 4 reagent boxes are connected to the U-RDU. Perform the relevant procedure according to your environment.
1.4.1 Preparing for switching of reagent tube connections Prepare the following items before switching tube connections. • Protective gloves • Paper or cloth to wipe reagent • Scissors for cutting reagent tube • Changeover wiring cord (Wiring cord_Assy No. 9695)
At the time of U-RDU installation, we provide you with a set of parts to be used in the event of a U-RDU failure. Prepare sufficient wiring cord for the number of instruments connected to the U-RDU. If you are connecting 6 instruments, prepare 6 changeover wiring cords. • Spout kit for UF-CELLSHEATH
At the time of U-RDU installation, we provide you with a set of parts to be used in the event of a U-RDU failure. Prepare 1 set if 5 instruments are connected to the U-RDU. Prepare 2 sets if 6 instruments are connected to the U-RDU.
1 Wear the protective gloves. 2 Turn OFF the power to the U-RDU and unplug the power cable from the U-RDU.
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3 Spread paper or a cloth on the floor behind the back of the U-RDU. Spread paper or a cloth on the floor behind the back of the U-RDU in case leakage occurs while you are working.
1.4.2
Changing the instrument settings
1 Touch the [Settings] icon on the [Menu] screen. The [Setting Menu] screen appears.
[Setting Menu] screen
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2 Touch the [System] icon. The [System Settings] screen appears.
[System Settings] screen
3 Touch the [U-RDU] icon. The [U-RDU Connection Settings] dialog box appears.
[U-RDU Connection Settings] dialog box
4 Uncheck the [Connect to U-RDU] checkbox. 5 Touch [Register]. The dialog box closes and the instruments shut down.
6 Ensure the instrument(s) has powered down before continuing.
Information After verifying that the power of the instruments is OFF, change the reagent tube connection. Do not turn on the power of any instruments while performing this work. Because reagent cannot be supplied to the instruments, an out-of-reagent error will occur.
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1.4.3
Troubleshooting
Closing the reagent tubes on the U-RDU side
Use the roller clamps to close the reagent tubes.
1 Close all roller clamps on the reagent tubes connected to "ANALYZER 1" to "ANALYZER 6" and "REAG #1" to "REAG #4" on the back of the U-RDU.
Note: To open/close the reagent tube, roll the wheel on the roller clamp. Open
Close
Roll the wheel until the yellow tube is completely closed. The figure below shows the connection positions of the reagent tubes.
(1) (3) (5)
(2) (4) (7) (6)
Yellow tube
(9)
(8)
(10)
(1) ANALYZER 1 (2) ANALYZER 2 (3) ANALYZER 3 (4) ANALYZER 4 (5) ANALYZER 5 (6) ANALYZER 6 (7) REAG #1 (8) REAG #2 (9) REAG #3 (10) REAG #4
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1.4.4 Changing "ANALYZER 1" to "ANALYZER 4" tube connections Remove the reagent tube that is connected to the U-RDU, and connect the tubing directly to the instrument.
Caution! Be careful not to spill reagent. If reagent is spilled, wipe up immediately with paper or a cloth. Risk of staining and deterioration of the instrument surface.
1 Remove the "REAG #1" yellow tube from the back of the U-RDU.
Yellow tube
2 Disconnect the white connector from the "ANALYZER 1" yellow tube. The yellow tube and roller clamp will remain attached to the U-RDU.
Yellow tube
White connector
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3 Connect the white connector you removed in step 2 to the yellow "REAG #1" tube you removed in step 1. Insert the yellow tube all the way in.
4 Repeat steps 1 to 3 to change the connections of the "ANALYZER 2" to "ANALYZER 4" tubes. To ensure that you do not mix up the tubes, repeat steps 1 to 3 individually for each ANALYZER 2 to 4. The ANALYZER and REAG combinations whose connections are changed are as follows. • ANALYZER 2 - REAG #2 • ANALYZER 3 - REAG #3 • ANALYZER 4 - REAG #4
5 Prepare the "A1" air bubble release line of the instrument. Prepare the "A1" air bubble release line that was connected in advance to the instrument during U-RDU installation.
6 Remove the black rubber joint connected to "AIR-REAG #1" from the U-RDU, and promptly connect to the "A1" air bubble release line of the instrument.
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7 Repeat steps 5 to 6 to change the connections of the "AIR-REAG #2" to "AIR-REAG #4" tubes. To ensure that you do not mix up the air bubble release lines, perform the changeover work one AIR-REAG #2 to 4 at a time. The AIR-REAG and air bubble release line combinations whose connections are changed are as follows. • AIR-REAG #2 - Air bubble release line A2 • AIR-REAG #3 - Air bubble release line A3 • AIR-REAG #4 - Air bubble release line A4
1.4.5 Changing "ANALYZER 5" and "ANALYZER 6" tube connections Prepare new reagent, and connect the tubing to the remaining instruments.
1 Attach the spout kit to the new reagent container.
2 Cut the transparent tube at a point about 3 cm from the white connector that is connected to the "ANALYZER 5" yellow tube with scissors.
About 3 cm
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3 Connect the cut transparent tube to the lower opening on the spout kit you prepared.
4 Prepare the instrument's "A5" air bubble release line. Prepare the "A5" air bubble release line that was connected in advance to the instrument during U-RDU installation.
5 Connect the "A5" air bubble release line of the instrument to the upper opening on the spout kit.
Instrument
6 Repeat steps 1 to 5 to change the connection of the "ANALYZER 6" tube. Use air bubble release line "A6" for "ANALYZER 6".
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1.4.6 Changing the wiring cord of "ANALYZER 1" to "ANALYZER 4" Remove the reagent wiring cord that is connected to the U-RDU, and connect the wiring cord directly to the instrument.
1 Prepare changeover wiring cord (Wiring cord_Assy No. 9695). Prepare a sufficient count for the number of instruments to be changed.
Small connector
Large connector
2 Remove the "ANALYZER 1" connector on the back of the U-RDU, and connect to the small connector on the changeover wiring cord.
Press in until you hear a "click" sound
3 Remove the "REAG #1" connector on the back of the U-RDU, and connect to the large connector on the changeover wiring cord.
Press in until you hear a "click" sound
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4 Repeat steps 1 to 3 to change the wiring cord of "ANALYZER 2" to "ANALYZER 4". To ensure that you do not mix up the wiring cord, repeat steps 1 to 3 individually for each ANALYZER 2 to 4. The ANALYZER and REAG combinations whose connections are changed are as follows. • ANALYZER 2 - REAG #2 • ANALYZER 3 - REAG #3 • ANALYZER 4 - REAG #4
1.4.7
Changing "ANALYZER 5" and "ANALYZER 6" wiring cord
Prepare new reagent, and connect the wiring cord to the remaining instruments.
1 Prepare the wiring cord of the "ANALYZER 5" reagent spout kit.
2 Prepare changeover wiring cord (Wiring cord_Assy No. 9695). Prepare sufficient wiring for the number of instruments connected to the U-RDU.
Small connector
Large connector
3 Remove the "ANALYZER 5" connector on the back of the U-RDU, and connect to the small connector on the changeover wiring cord.
Press in until you hear a "click" sound
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4 Connect the connector you readied in step 1 to the large connector on the changeover wiring cord.
Press in until you hear a "click" sound
5 Repeat steps 1 to 4 to change the wiring cord of "ANALYZER 6".
1.4.8 Opening the roller clamps on "REAG #1" to "REAG #4" When you have finished changing all tubes and wiring cord, open the roller clamps connected to the "REAG #1" to "REAG #4" spout kits to restore reagent flow.
Information Do not loosen the roller clamps of "ANALYZER 1" to "ANALYZER 6" connected to the U-RDU. Risk of reagent leakage.
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1.4.9
Troubleshooting
Starting the instruments
1 Before starting each instrument, check the analysis line. If a sample rack remains on the analysis line, remove the rack.
2 Turn on the instrument power. If an error occurs on an instrument after you start the instrument, touch the screen to stop the alarm.
1.4.10
Registering instrument reagent information
1 Register the reagent information of the UF-CELLSHEATH connected to each instrument. (➤P.82 "Chapter 2: 2.13 Replacing reagents") As an example, the reagent connected to "ANALYZER 1" is indicated on the spout kit tag as A1 or #1. In the same way, register reagent information for the other instruments so that the combination of ANALYZER and spout kit tag numbers is the same. If the hand-held barcode reader does not extend to the reagent box, enter the Reagent Code manually. When you have completed replacement of all reagents, the instrument is ready for use.
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Chapter 2
Maintenance
Chapter 2 Maintenance This chapter explains an overview of instrument maintenance and those procedures, replacing and replenishing supplies, and checking instrument operation.
2.1
List of maintenance tasks
Regular maintenance is necessary to keep the instrument in optimal condition. When the instrument is in continuous use, perform "Daily maintenance" once every 24 hours. Perform the maintenance on the instrument according to the following schedule. Record the performed maintenance tasks on the instrument using the maintenance inspection checklist. (➤P.95 "2.16 Maintenance inspection checklist")
Risk of infection Be sure to wear adequate personal protective equipment, such as protective gloves, a protective mask, protective eyewear, and a lab coat when working. After completion of work, wash hands with disinfectant. There is a risk of infection by pathogens or other contaminates.
Maintenance tasks Daily maintenance
Shutting down or restarting the instrument
Pages ➤Basic Operation P.12
Checking the volume of fluid in the trap chamber
➤P.79
Weekly maintenance
Cleaning the rinse bottle
➤P.78
Maintenance as needed
Background check
➤P.68
Cleaning
➤P.69
Rinsing the flowcell
➤P.70
Replenishing rinse water
➤P.77
Removing fluid from the trap chamber
➤P.79
Disposing of waste fluid*
➤P.81
Replacing the reagents
➤P.82
Rinsing/replacing the sample filter
➤P.87
Replacing the fuses
➤P.91
* Only when using the waste tank (optional).
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Maintenance
[Maintenance Menu] screen
Touch the [Maintenance] icon on the [Menu] screen to display the [Maintenance Menu] screen. Maintenance tasks and operation tests of the instrument can be performed from the [Maintenance Menu] screen.
[Maintenance Menu] screen [Status Disp.]
Touch to check the pressure, temperature, and other statuses of each unit in the instrument. (➤P.65 "2.3 Checking the status of the instrument")
[Counter]
Touch to check the analysis count in each analysis mode, and the operation count for each unit. (➤P.67 "2.4 Checking the operation count (counter)")
[Background Check] Touch to perform a background check. (➤P.68 "2.5 Performing a background check") [Rinse]
Touch to perform instrument cleaning. (➤P.69 "2.6 Performing cleaning")
[Rinse Flowcell]
Touch to rinse the flowcell. (➤P.70 "2.7 Rinsing the flowcell")
[Operation Test]
Touch to perform operation tests of each unit in the instrument. (➤P.72 "2.8 Performing instrument operation tests")
[Sample filter]
Touch to rinse or replace the sample filter. (➤P.87 "2.14 Rinsing/replacing the sample filter")
[Back]
Touch to return to the [Menu] screen.
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Maintenance
Checking the status of the instrument
You can check the status of instrument such as temperature and pressure of each unit. The information displayed is updated every second.
1 Touch the [Status Disp.] icon on the [Maintenance Menu] screen. The [Status Disp.] dialog box appears. Tabs
2 Touch the tab for the instrument status that you want to check. [Pressure/Temp. Laser Current] tab The following information is displayed on the [Pressure/ Temp. Laser Current] tab. Any values outside the specified range are displayed in white characters on a red background.
[Status Disp.] dialog box
Note: When an error related to pressure or temperature control occurs, the [Status Disp.] dialog box can also be displayed by the steps below. (1) Select the error related to pressure or temperature control in the error message list in the [ErrorHelp] dialog box. (2) Touch [Status Disp.].
[Pressure]
Displays the pressure of each unit in the instrument.
[Laser Current]
Displays the output current of the laser.
[Temperature]
Displays the temperature of each unit in the instrument and the ambient temperature.
[SV (Analyzer)]/[Sensor (Analyzer)] tab The operational status of solenoid valves and sensors in the analyzer is displayed. Sensors that are ON are shown in red. Sensors that are OFF are shown in white.
e.g. [SV (Analyzer)] tab
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[Sensor (Sampler)] tab The operating state of sensors in the sampler is displayed. This does not appear when the sampler (CV-11) is connected. Sensors that are ON are shown in red. Sensors that are OFF are shown in white.
3 Check the details in the dialog box, and touch [Close]. The dialog box closes and return to the [Maintenance Menu] screen.
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Maintenance
Checking the operation count (counter)
You can check the analysis count in each analysis mode, and the operation count for each unit.
1 Touch the [Counter] icon on the [Maintenance Menu] screen. The [Counter] dialog box appears.
[Counter] dialog box [Test Counts]
Displays the analysis count in each analysis mode. In [Maintenance Measurement], the background check count (including the background check for body fluid analysis) is displayed.
[Operation Counts]
Displays the operation count by unit.
2 Check the details in the dialog box, and touch [Close]. The dialog box closes and return to the [Maintenance Menu] screen.
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2.5
Maintenance
Performing a background check
A background check is the process of rinsing hydraulic parts in the instrument and performing analysis without sample aspiration to verify the effectiveness of rinsing. While a background check is performed automatically at the instrument startup, perform a background check manually when the accuracy of analysis results is suspected, or after replacing a reagent.
1 Make sure the instrument is in the ready state for sampler analysis or STAT analysis. If the instrument is not in the ready state, wait until the instrument status becomes [Sampler Ready], [Sampler Int. Ready], or [Stat Ready].
2 Touch the [Background Check] icon on the [Maintenance Menu] screen. The confirmation dialog box appears.
3 Touch [Yes]. The [Background Check] dialog box appears, and the background check starts. Progress is shown in a progress bar at the bottom of the dialog box. The background check may takes up to 4 minutes. Wait until it is complete.
[Background Check] dialog box
4 Check the results. Check the result of the background check in the [Background Check] dialog box. Any values out of the acceptable range are displayed in white characters on a red background. If there is an abnormality in the result, perform cleaning. For details, see the following. (➤P.69 "2.6
Performing cleaning")
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Maintenance
Performing cleaning
Cleaning is the process of rinsing the hydraulic parts and pipette. Cleaning is automatically performed when shutdown is executed. You can also perform cleaning without shutdown to restore the instrument to the same condition as after startup.
1 Make sure the instrument is in the ready state for sampler analysis or STAT analysis. If the instrument is not in the ready state, wait until the instrument status becomes [Sampler Ready], [Sampler Int. Ready], or [Stat Ready].
2 Touch the [Rinse] icon on the [Maintenance Menu] screen. The confirmation dialog box appears.
3 Touch [Yes]. The dialog box closes and cleaning starts. Progress is shown in a progress bar on the status area at the bottom of the screen. Cleaning takes about 10 minutes. Wait until it is complete.
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Maintenance
Rinsing the flowcell
If the flowcell clogs or is dirty, perform flowcell rinsing.
Warning! CELLCLEAN U is a strong alkaline detergent. Be sure to wear adequate personal protective equipment, such as protective gloves, a protective mask, protective eyewear, and a lab coat when handling the reagent. In the event that CELLCLEAN U comes into contact with your body or clothes, rinse immediately with copious amounts of running water. Risk of skin injury and damage to your clothes.
1 Make sure the instrument is in the ready state for sampler analysis or STAT analysis. If the instrument is not in the ready state, wait until the instrument status becomes [Sampler Ready], [Sampler Int. Ready], or [Stat Ready].
2 Touch the [Rinse Flowcell] icon on the [Maintenance Menu] screen.
3 Dispense CELLCLEAN U into a sample tube or sample cup. The amount of CELLCLEAN U needed to rinse the flowcell is about 2.2 mL.
Note: When using a sample cup, set the sample cup in adapter No. 368.
The [Rinse Flowcell] dialog box appears. Sample cup
Adapter No. 368
4 Set the sample tube or sample cup into [Rinse Flowcell] dialog box
the STAT sample holder.
When the sampler (SA-51) is connected, the STAT sample tube holder automatically slides out.
STAT sample holder
e.g. Sampler (SA-51) (Regular sample tube)
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5 Push in the STAT sample tube holder. Be sure to push in until the STAT sample tube holder is locked.
Maintenance
8 Remove the CELLCLEAN U from the STAT sample holder.
e.g. Regular sample tube
6 Touch [Execute]. CELLCLEAN U is aspirated and rinsing starts. When rinsing starts, the progress dialog box appears. Progress is indicated by a progress bar.
9 When the sampler (SA-51) is connected, push in the STAT sample tube holder. Be sure to push in the STAT sample tube holder until it is locked.
When the sampler (SA-51) is connected, once aspiration of the CELLCLEAN U is completed, the STAT sample tube holder automatically slides out. When the sampler (CV-11) is connected, the STAT sample tube holder automatically unlocks.
Note: If the STAT sample tube holder is not locked, [Execute] cannot be touched in the [Rinse Flowcell] dialog box. Push in the STAT sample tube holder again until it is locked.
7 When the sampler (CV-11) is connected, press the release button on the sampler (CV-11).
Caution! Do not push the sample tube or sample cup when pushing in the STAT sample tube holder. It may cause a malfunction of the instrument.
The STAT sample tube holder slides out.
10 Wait until the process finishes. Rinsing of the flowcell takes about 8 minutes.
Release button
When flowcell rinsing finishes, the progress dialog box closes.
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2.8
Maintenance
Performing instrument operation tests
The operation of each unit can be tested. These procedures can be used to identify the cause of an error. Analysis is not possible while an operation test is in progress. To perform an operation test, the instrument must be in the ready state for sampler analysis. Follow the steps below to switch the instrument to the ready state for sampler analysis.
1 Make sure the instrument is in the ready state for sampler analysis. If the instrument is not in the ready state, wait until the instrument status becomes [Sampler Ready] or [Sampler Int. Ready].
● If the sampler analysis button appears as shown on the right Touch the sampler analysis button and touch [Yes] in the dialog box that appears. Sampler analysis button
2.8.1 [Operation Test menu] screen
2.8.2
Sampler operation test
Touch the [Operation Test] icon on the [Maintenance
When an error related to sampler operation occurs,
Menu] screen to display the [Operation Test menu]
perform a sampler operation test to identify the cause of
screen. To perform an operation test, select the unit on
the error.
the [Operation Test menu] screen.
The sampler operation test can only be performed when the sampler (SA-51) is connected.
1 Make sure the instrument is in the ready state for sampler analysis.
2 Touch the [Sampler] icon on the [Operation Test menu] screen. The following dialog box appears.
[Operation Test menu] screen
Note: When the sampler (CV-11) is connected, the icons below do not appear. • [Sampler] • [Barcode Reader] [Front Belt Sampler Operation Test]
Perform a front sampler belt operation test.
[Back Belt Sampler Operation Test]
Perform a back sampler belt operation test.
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3 Set a sample rack in the right sampler pool. Slide the groove on the rack onto the protrusion on the right side, as viewed from the front of the analyzer.
Maintenance
7 Remove the sample rack. When the operation test finishes, the rack is conveyed to the left sampler pool. When removing a rack, check that the protrusion has cleared the groove, and then remove the rack.
e.g. Sampler (SA-51)
Caution! • Load the rack horizontally with respect to the instrument. If loaded at an angle, correct operation cannot be assured. • Check that no dirt or foreign objects are on the left and right sampler pools and the analysis line. Also check that no dirt or foreign objects are stuck to the bottom of the rack and that the rack is not damaged or deformed. • Ensure there are no obstacles impeding rack movement. For Example, the cable from a handheld barcode reader. • Do not bubble the sample. Incorrect analysis results may be obtained if the sample has bubbles.
4 Select the belt to be tested and touch [Execute]. The operation test starts and the progress dialog box appears. Wait until it is complete. When the operation test finishes, the progress dialog box closes and the finish dialog box appears. If an error occurred during the test, an error message appears. (➤P.7 "Chapter 1: 1.1 alphabetical order)")
Error message list (in
5 Touch [OK]. The finish dialog box closes.
6 Touch [Cancel]. The dialog box closes.
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2.8.3 Barcode reading test When an error related to the barcode reader occurs, perform a barcode reading test to identify the cause of the
4 Set a sample rack in the right sampler pool.
error. The barcode reading test can only be performed when the sampler (SA-51) is connected.
1 Make sure the instrument is in the ready
• Slide the groove on the rack onto the protrusion on the right side, as viewed from the front of the analyzer. • The barcode on the sample tubes should face the inside of the instrument.
state for sampler analysis.
2 Touch the [Barcode Reader] icon on the [Operation Test menu] screen. The following dialog box appears.
e.g. Sampler (SA-51)
Caution! [Tube Pos.]
Displays the tube position.
[Sample No.] Displays the sample number that was read from the barcode. [Check Digit] Displays the check digit of the barcode. [Barcode type]
Displays the barcode type.
[Results]
Displays the reading result. One of the following symbols appears depending on the result. If there were no reading problems, nothing appears. [E]: Barcode reader read error or incorrect check digit. [+]: The value that was read is longer than the specified number of digits. [-]: The value that was read is shorter than the specified number of digits.
• Load the rack horizontally with respect to the instrument. If loaded at an angle, correct operation cannot be assured. • Check that no dirt or foreign objects are on the left and right sampler pools and the analysis line. Also check that no dirt or foreign objects are stuck to the bottom of the rack and that the rack is not damaged or deformed. • Ensure there are no obstacles impeding rack movement. For Example, the cable from a handheld barcode reader. • Do not bubble the sample. Incorrect analysis results may be obtained if the sample has bubbles.
3 Insert sample tubes with barcodes into the sample rack.
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5 Touch [Execute]. The rack is conveyed and the operation test begins. As each barcode is read, the result appears in the dialog box.
Warning! Do not insert your hand in the analysis line while the instrument is in operation. Doing so may result in injury.
Maintenance
2.8.4 Sheath syringe operation test When an error related to sheath syringe operation occurs, perform a sheath syringe operation test to identify the cause of the error.
1 Make sure the instrument is in the ready state for sampler analysis.
2 Touch the [Sheath Syringe] icon on the [Operation Test menu] screen. The confirmation dialog box appears.
Note: If you want to add a sample rack while the operation test is in progress, insert sample tubes in the rack and place the rack in the right sampler pool.
6 Touch [Cancel]. The dialog box closes.
7 Remove the sample rack. When the operation test finishes, the rack is conveyed to the left sampler pool. When removing a rack, check that the protrusion has cleared the groove, and then remove the rack.
3 Touch [Yes]. The operation test starts and the progress dialog box appears. Wait until it is complete. When the operation test finishes, the progress dialog box closes and the finish dialog box appears. If an error occurred during the test, an error message appears. (➤P.7 "Chapter 1: 1.1 alphabetical order)")
Error message list (in
4 Touch [OK]. The finish dialog box closes.
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2.8.5 Pump operation test
2.8.6
Arm operation test
When an error related to pump operation occurs, perform
When an error related to arm operation occurs, perform
a pump operation test to identify the cause of the error.
an arm operation test to identify the cause of the error.
1 Make sure the instrument is in the ready
1 Make sure the instrument is in the ready
state for sampler analysis.
2 Touch the [Charging Pump]/[Urine
state for sampler analysis.
2 Touch the [Sample Arm]/[Dispensing
Pump]/[Dispensing Pump] icon on the [Operation Test menu] screen.
Arm] icon on the [Operation Test menu] screen.
The confirmation dialog box appears.
The confirmation dialog box appears.
3 Touch [Yes].
3 Touch [Yes]. The operation test starts and the progress dialog box appears. Wait until it is complete.
The operation test starts and the progress dialog box appears. Wait until it is complete.
When the operation test finishes, the progress dialog box closes and the finish dialog box appears.
When the operation test finishes, the progress dialog box closes and the finish dialog box appears.
If an error occurred during the test, an error message appears.
If an error occurred during the test, an error message appears.
(➤P.7 "Chapter 1: 1.1 alphabetical order)")
(➤P.7 "Chapter 1: 1.1 alphabetical order)")
Error message list (in
4 Touch [OK]. The finish dialog box closes.
Error message list (in
4 Touch [OK]. The finish dialog box closes.
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2.9
Maintenance
Replenishing rinse water
Check the rinse water in the bottle and replenish if low.
Caution! Do not use anything OTHER than purified water for the rinse water. Risk of instrument failure and inaccurate analysis results.
1 Remove the cap from the rinse water bottle.
3 Replenish the rinse water. Fill the rinse water bottle with rinse water until it is full.
Turn the cap in the direction of the arrow to remove it.
4 Insert the spout kit straight into the rinse bottle.
5 Close the cap of the rinse bottle. Close the cap by turning it in the opposite direction of step 1.
6 Touch the [Reagents Replacement] icon on the [Menu] screen.
2 Pull out the spout kit straight up.
The [Reagent] dialog box appears. (➤P.82 "2.13.1 [Reagent] dialog box")
7 Touch [Rinse Water]. 8 Touch [Register]. The progress dialog box appears and replenishment of rinse water starts. Replenishment takes approximately 2 minutes. The progress dialog box closes when replenishment is complete, and the information displayed in the [Reagent] dialog box is updated.
Caution! Do not touch the aspiration nozzle of the spout kit. Keep dust, dirt, bacteria, and other contaminants away from the aspiration nozzle and the inside of the container.
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2.10
Maintenance
Cleaning the rinse bottle
Wash the rinse bottle once a week. Wash as needed whenever the rinse bottle becomes dirty.
1 Remove the cap from the rinse water bottle.
5 Replenish the rinse water. Fill the rinse water bottle with rinse water until it is full.
Turn the cap in the direction of the arrow to remove it.
Caution! Do not use anything OTHER than purified water for the rinse water. Risk of instrument failure and inaccurate analysis results.
6 Insert the spout kit straight into the rinse bottle.
7 Close the cap of the rinse bottle. 2 Pull out the spout kit straight up.
Close the cap by turning it in the opposite direction of step 1.
Caution! Do not touch the aspiration nozzle of the spout kit. Keep dust, dirt, bacteria, and other contaminants away from the aspiration nozzle and the inside of the container.
3 Discard the rinse water in the bottle. 4 Use tap water to wash the inside of the bottle and the spout kit, and then rinse them with rinse water.
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2.11
Maintenance
Checking the volume of fluid and removing fluid in the trap chamber
When finishing analysis for the day, check the volume of fluid in the trap chamber, and remove any that has collected.
Risk of infection When disposing of fluid in the trap chamber, be sure to wear adequate personal protective equipment, such as protective gloves, a protective mask, protective eyewear, and a lab coat. After completion of work, wash hands with disinfectant. There is a risk of infection by pathogens or other contaminates.
Caution! If the trap chamber collects fluid even after taking the remedial action, the analyzer may have malfunctioned. Contact your local Sysmex service representative.
1 Open the front upper cover. Open up the cover until it is locked.
Caution! When the front lower cover unlocks, it falls forward. When unlocking the front lower cover, press the upper part of the cover with your hand to keep the cover from falling.
Front upper cover
2 Lift up the front lower cover and remove. Lift up the front lower cover so that the tabs (x2) on the analyzer separate from the notches on the bottom of the cover.
2 Remove the front lower cover. 1 Press the lock buttons (x2) on the front lower cover. The front lower cover unlocks.
Tabs
Lock buttons Front lower cover
e.g. Sampler (SA-51)
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3 Check if any fluid has collected in the trap chamber.
6 Attach the front lower cover. 1 Attach the front lower cover to the analyzer. First insert the tabs on the analyzer into the notches (x2) on the bottom of the front lower cover.
Trap chamber
If fluid has collected, go to step 4. If no fluid has collected, the following steps are not necessary. Attach the front lower cover. For details on attaching the front lower cover, see step 6.
4 Turn the trap chamber in the direction of the arrow to remove it, and dispose of the fluid collected in the chamber.
Tabs
Then align the upper part of the front lower cover with the analyzer, and insert the tabs (x2) on the inner side of the lock buttons into the holes on the upper part of the analyzer.
2 Press in the lock buttons (x2) on the front lower cover. The front lower cover locks.
Caution! When locking the front lower cover, press the upper part of the cover with your hand.
Float
Caution! • Be careful not to dispose of the float. • Hold the removed float in the same orientation and insert it straight into the trap chamber.
Lock buttons Front lower cover
5 Install the trap chamber by turning it in the direction opposite to that of step 4.
7 Close the front upper cover. Gently press down on the cover to release the lock, and close the cover.
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2.12
Maintenance
Disposing of waste fluid in the waste tank
When using the waste tank (optional), an error occurs when the tank is full. Dispose of the waste fluid in the tank immediately.
Risk of infection • Be sure to wear adequate personal protective equipment, such as protective gloves, a protective mask, protective eyewear, and a lab coat when disposing of waste fluid. After completion of work, wash hands with disinfectant. There is a risk of infection by pathogens or other contaminates. • Waste fluid and other potentially infected items must be separated from general waste and treated according to applicable local and national regulations.
1 Make sure the instrument is in the ready state for sampler analysis or STAT analysis. If the instrument is not in the ready state, wait until the instrument status becomes [Sampler Ready], [Sampler Int. Ready], or [Stat Ready].
Risk of infection Do not dispose of waste fluid while the instrument mechanisms are in motion, such as during analysis. Waste fluid could spray out of the tube and onto your skin or clothes, causing a risk of infection by pathogens or other contaminates.
4 Rinse the waste tank. 5 Disinfect with alcohol the inside of the waste tank, the cap, and any parts that were in contact with waste fluid.
6 Close the cap of the waste tank. Caution! Place the waste tank below the bottom of the instrument.
2 Remove the cap from the waste tank. Turn the cap in the direction of the arrow to remove it.
3 Dispose of the waste fluid.
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2.13
Maintenance
Replacing reagents
When a reagent runs out or expires, an error message appears and analysis is suspended. Replace the reagent immediately.
2.13.1 [Reagent] dialog box Touch the [Reagents Replacement] icon on the [Menu]
The [Reagent] dialog box shows information on each
screen to display the [Reagent] dialog box. The [Reagent]
reagent.
dialog box allows you to check the status of reagents and
Expiration date
to replace reagents.
Lot number Reagent status Remaining volume graph Reagent name
[Reagent] dialog box
Expiration date*
Displays the expiration date of the reagent. This is not displayed unless the reagent information is registered. If expired, the reagent is displayed in white characters on a red background.
Lot number*
Displays the lot number of the reagent.
Reagent status
Displays the reagent status. Some status may not appear depending on the reagent type.
Note: • The [Reagent] dialog box can also be displayed by touching the indicator for reagent status on the status area at the bottom of the screen. Reagent status
• When an error related to reagents occurs, the [Reagent] dialog box can also be displayed by the steps below. (1) Select the error related to reagents in the error message list in the [ErrorHelp] dialog box. (2) Touch [Reagent Replace].
[residue < (remaining count)] / [residue < (remaining volume)]
The reagent is low.
[Replace]/ [Empty]
The reagent has expired or run out. [Empty] is displayed for rinse water.
[Unregistered] The information of the set reagent is not registered. [Received]
Registration of the reagent information is complete.
Remaining volume graph
Volume of reagent is displayed as a graph. This is not displayed when the reagent is not registered or runs out.
Reagent name
Displays the reagent name.
* Does not appear for rinse water.
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2.13.2
Maintenance
Replacing diluent and sheath fluid
When replacing UF-CELLSHEATH, UF-CELLPACK CR, or UF-CELLPACK SF, follow the steps below.
Caution! • Do not add new reagent to existing reagent. • Place the reagent at a level no more than 1 meter above or below the bottom of the analyzer. Do not put reagents on top of the instrument. • The new reagent must be left undisturbed for over 24 hours at 10 to 30 °C before using. • Take care not to spill reagent. In case of spillage, wipe immediately with a damp cloth. Otherwise, remained reagent on the surface of instrument may cause staining or deterioration. • Read the indication of the reagent container or package box, Safety Data Sheet, and "General Information" before handling reagents. (➤General Information "Chapter 7 Reagents")
1 Prepare new reagent. Check that the reagent has not expired.
Note: When entering the Reagent Code manually, touch the reagent to be replaced in the [Reagent] dialog box. The following dialog box appears.
Enter the [Reagent Code], and touch [Register]. If the dialog box for entering a lot number appears, follow the instructions in the dialog box to register the reagent information.
4 Remove the cap from the new reagent container.
2 Display the [Reagent] dialog box. (➤P.82 "2.13.1
[Reagent] dialog box")
3 Enter the Reagent Code of the new reagent. Enter by barcode reading Read the Reagent Code (barcode) of the new reagent with hand-held barcode reader. The Reagent Code is located on the outer box of sheath fluid and on the container of diluent. Reagent Code is as shown below.
When the Reagent Code is entered, the reagent in the [Reagent] dialog box displays [Received]. The expiration date and the lot number of the new reagent also appear.
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5 Remove the cap from the old reagent container.
6 Pull out the float switch/spout kit straight up.
For UF-CELLSHEATH, turn lower part of the cap in the direction of the arrow to remove it.
For UF-CELLSHEATH
For UF-CELLSHEATH For UF-CELLPACK CR/UF-CELLPACK SF, turn the cap in the direction of the arrow to remove it.
For UF-CELLPACK CR/UF-CELLPACK SF
Caution! For UF-CELLPACK CR/UF-CELLPACK SF
Do not touch the aspiration nozzle of the float switch/ spout kit. Keep dust, dirt, bacteria, and other contaminants away from the aspiration nozzle and the inside of the container.
7 Insert the float switch/spout kit straight into the new reagent container and close the cap. Close the cap by turning it in the opposite direction of step 5.
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8 Touch [Replace] in the [Reagent] dialog box.
Maintenance
3 Open the front upper cover. Open up the cover until it is locked.
The progress dialog box appears, and reagent replacement starts. The progress dialog box closes when reagent replacement is complete, and the information displayed in the [Reagent] dialog box is updated.
Front upper cover
The time guidelines for reagent replacement are as shown below.
Reagent name UF-CELLSHEATH
Time Approx. 1 minute
UF-CELLPACK CR
Approx. 2 minutes
UF-CELLPACK SF
Approx. 2 minutes
4 Pull up the cover from the dye that is to be replaced. Pull up the cover until it clicks.
2.13.3
Replacing dye
When replacing UF-Fluorocell CR or UF-Fluorocell SF, follow the steps below.
Caution! • Be sure to wear adequate personal protective equipment, such as protective gloves, a protective mask, protective eyewear, and a lab coat when replacing the reagent. • Do not add new reagent to existing reagent. • Read the labels on the reagent containers and/or boxes, the Instructions for Use and the Safety Data Sheet, and "General Information" before handling reagents. (➤General Information "Chapter 7 Reagents")
e.g. UF-Fluorocell CR
The position to attach dye is shown below.
SF
CR
Information Do not replace dye while the instrument is in operation. If you replace dye during operation, the RFID tag will be damaged, and the information will be unable to be read properly.
5 Remove the old reagent cartridge from 1 Prepare a new reagent cartridge.
its holder.
Check that the reagent has not expired.
Reagent cartridge
2 Display the [Reagent] dialog box. (➤P.82 "2.13.1
[Reagent] dialog box")
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6 Attach the new reagent cartridge into the holder. Analyzer beeps.
Caution! • If you attach a wrong reagent, an error occurs and an alarm sounds. Check the error and attach the correct reagent. • Take care not to spill reagent. In case of spillage, wipe immediately with a damp cloth. Otherwise, the coated surface of the instrument could be stained or deteriorated.
7 Pull down the cover on the dye. Reagent replacement is executed. The expiration date and lot number of the new reagent appears in the [Reagent] dialog box.
8 Close the front upper cover. Gently press down on the cover to release the lock, and close the cover.
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2.14
Maintenance
Rinsing/replacing the sample filter
If the sample filter is clogged or dirty, the specified amount of sample cannot be aspirated and an error occurs. When an error occurs, rinse or replace the sample filter.
Risk of infection Be sure to wear adequate personal protective equipment, such as protective gloves, a protective mask, protective eyewear, and a lab coat when rinsing or replacing the sample filter. After completion of work, wash hands with disinfectant. There is a risk of infection by pathogens or other contaminates.
Warning! Be sure to shut down the instrument before rinsing or replacing the sample filter. Otherwise, there is a risk of electrical shock.
1 Make sure the instrument is in the ready state for sampler analysis or STAT analysis. If the instrument is not in the ready state, wait until the instrument status becomes [Sampler Ready], [Sampler Int. Ready], or [Stat Ready].
6 Open the front upper cover. Open up the cover until it is locked. Front upper cover
2 Touch the [Maintenance] icon on the [Menu] screen. The [Maintenance Menu] screen appears. (➤P.64 "Chapter 2: 2.2 screen")
[Maintenance Menu]
3 Touch the [Sample filter] icon. The confirmation dialog box appears.
4 Touch [Execute]. The sample filter starts moving, and the progress dialog box appears.
7 Remove the front lower cover. 1 Press the lock buttons (x2) on the front lower cover. The front lower cover unlocks.
When the sample filter has moved to the replacement position, the dialog box closes, and the power to the instrument automatically turns OFF.
5 Wait 1 minute to allow the pressure to fall inside the instrument. Lock buttons Front lower cover
e.g. Sampler (SA-51)
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9 Remove the tube adapters from the top Caution! When the front lower cover unlocks, it falls forward. When unlocking the front lower cover, press the upper part of the cover with your hand to keep the cover from falling.
and bottom of the sample filter. Turn the top and bottom tube adapters in the direction of the arrow to remove them. Tube adapter (top)
2 Lift up the front lower cover and remove. Lift up the front lower cover so that the tabs (x2) on the analyzer separate from the notches on the bottom of the cover.
Tube adapter (bottom)
Caution! Tabs
• Do not forcibly pull the tube when removing the tube adapter. • Do not bend the tube. This is necessary to avoid the damage to the tube.
8 Remove the sample filter case from the holder.
10 Remove the cap from the sample filter case.
Warning!
Turn the cap in the direction of the arrow to remove it. Cap
Have a dry cloth ready in case fluid leaks from the tube when removing the sample filter. The risk of electrical shock is high when your hands are wet.
Holder
Caution! Be careful not to drop the filter inside the case when removing the cap from the sample filter case.
Sample filter case
Filter
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11 Remove the filter from the sample filter
Maintenance
13 Install the tube adapters onto the top
case, and rinse it with purified water.
and bottom of the sample filter.
After rinsing, use a dry cloth to dry the filter gently. Check whether or not there is clogging in the hole, and rinse the filter again if there is clogging. If the clogging cannot be eliminated, replace the filter.
Install the top and bottom tube adapters by turning them in the opposite direction of step 9 with the cap up as shown. Tube adapter (top) Cap
Filter
Tube adapter (bottom)
14 Install the sample filter case into the
Caution! If there is a clog in the sample filter, this may have affected analysis count values obtained before the filter was rinsed or replaced. Check if there are any abnormalities in the analysis result data.
holder.
Sample filter case
12 Set the rinsed or new filter in the sample filter case, and attach the cap. Attach the cap by turning it in the opposite direction of step 10.
Holder
Close the cap firmly. Cap
Filter
15 Attach the front lower cover. 1 Attach the front lower cover to the analyzer. First insert the tabs on the analyzer into the notches (x2) on the bottom of the front lower cover.
Sample filter case
Tabs
Then align the upper part of the front lower cover with the analyzer, and insert the tabs (x2) on the inner side of the lock buttons into the holes on the upper part of the analyzer.
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2 Press in the lock buttons (x2) on the front lower cover.
● When the sampler (CV-11) is connected Press the startup switch on the sampler (CV-11).
The front lower cover locks.
Caution! When locking the front lower cover, press the upper part of the cover with your hand.
Startup switch
Log on to the instrument after startup.
Lock buttons Front lower cover
18 Perform QC analysis. For details on QC analysis, see "Basic Operation". (➤Basic Operation "Chapter 2: 2.6 Performing QC analysis")
16 Close the front upper cover. Gently press down on the cover to release the lock, and close the cover.
17 Turning ON the power to the instrument. ● When the sampler (SA-51) is connected
Check the following points, and reassemble the sample filter if there is a problem. • A short sample error does not occur • There is no fluid leakage from the filter • There is no abnormality with the QC analysis results
Press the power switch on the sampler (SA-51).
Power switch
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2.15
Maintenance
Replacing the fuses
Over-current protection fuses are used in the instrument, pneumatic unit and sampler (CV-11). If a fuse blows, replace the fuse immediately.
Warning! • Make sure to unplug the power cable before replacing a fuse. Otherwise, there is a risk of electrical shock. • Use a fuse of the specified type and rating. Otherwise, an electrical malfunction could result causing smoke or a fire.
Information If the fuse blows again immediately after it is replaced, there may be a fault in the instrument. Contact your local Sysmex service representative.
Note: • The fuse that is used in the pneumatic unit varies by region/country. Contact your local Sysmex service representative. • For details about replacing the fuse on the sampler (CV-11), refer to the Instructions for Use for CV-11. Fuse used in the instrument Product number 26677681
2.15.1
Product name Fuse 50T100H
Rating 250 V 10 A
Type Time lag
Quantity 2
Replacing the fuse in the instrument
1 Shut down the instrument. 1 Make sure the instrument is in the
3 Touch the [Shutdown] icon. The confirmation dialog box appears.
4 Touch [Yes].
ready state for sampler analysis or STAT analysis.
Shutdown takes place and the instrument power automatically turns OFF.
If the instrument is not in the ready state, wait until the instrument status becomes [Sampler Ready], [Sampler Int. Ready], or [Stat Ready].
Shutdown takes approximately 10 minutes.
2 Touch the [Menu] button on the toolbar. The [Menu] screen appears.
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2 Turn OFF the main power switch on the back of the instrument.
2 Remove the old fuse from the fuse holder. Fuses
Fuse holder
6 Set the new fuse into the fuse holder, and insert it into the back of the instrument.
e.g. Sampler (SA-51)
3 Unplug the power cable from the power outlet.
4 Unplug the power cable from the back of the instrument.
7 Plug in the power cable on the back of the instrument.
8 Connect the power cable to the power outlet, and turn ON the main power switch on the back of the instrument.
9 Turning ON the power to the instrument. ● When the sampler (SA-51) is connected Press the power switch on the sampler (SA-51).
Plug Power switch
5 Remove the old fuses. 1 Use a flathead screwdriver to push up on the hook part of the fuse holder, and withdraw the fuse holder.
Fuse holder
Hook
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Maintenance
3 Unplug the power cable from the back bottom of the pneumatic unit.
Startup switch Plug
Log on to the instrument after startup.
2.15.2
Replacing the fuses in pneumatic unit
4 Remove the old fuses. 1 Use a flathead screwdriver to push up on the hook part of the fuse holder, and withdraw the fuse holder. Fuse holder
1 Shut down the instrument.
Hook
1 Make sure the instrument is in the ready state for sampler analysis or STAT analysis. If the instrument is not in the ready state, wait until the instrument status becomes [Sampler Ready], [Sampler Int. Ready], or [Stat Ready].
2 Touch the [Menu] button on the toolbar. The [Menu] screen appears.
3 Touch the [Shutdown] icon.
2 Remove the old fuses from the fuse holder.
The confirmation dialog box appears.
Fuses
4 Touch [Yes]. Shutdown takes place and the instrument power automatically turns OFF. Shutdown takes approximately 10 minutes.
Fuse holder
2 Unplug the power cable of the pneumatic unit from the power outlet.
5 Set new fuses into the fuse holder, and insert it into the pneumatic unit.
6 Plug in the power cable on the rear bottom of the pneumatic unit.
7 Connect the power cable of the pneumatic unit to the power outlet.
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8 Turning ON the power to the instrument. ● When the sampler (SA-51) is connected Press the power switch on the sampler (SA-51).
Power switch
● When the sampler (CV-11) is connected Press the startup switch on the sampler (CV-11).
Startup switch
Log on to the instrument after startup.
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UF-4000 Troubleshooting
1
4
8
Replacing the fuses (Instrument/pneumatic unit)
Replacing the reagent (UF-CELLPACK CR)
Rinsing the flowcell
M/D Signed
Replacing the reagent (UF-CELLPACK SF)
Replacing the reagent (UF-CELLSHEATH)
Maintenance task
* We recommend that our customers prepare a checklist that suits their operating environment.
Rinsing/replacing the sample filter
Disposing of waste fluid
Removing fluid from the trap chamber
M/D Signed
Reagents and supply parts replacement
Replacing the reagent (UF-Fluorocell CR)
Replenishing rinse water
Yr.
M/D Signed
9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
M/D Signed
7
Cleaning
6
Replacing the reagent (UF-Fluorocell SF)
M/D Signed
5
Background check
Maintenance task
3
M/D Signed
2
Maintenance as needed
Cleaning the rinse bottle
Maintenance task
Weekly maintenance
Signed
Checking the volume of fluid in the trap chamber
Shutting down or restarting the instrument
Day
Month
2.16
Maintenance task
Daily maintenance tasks
Example:
Chapter 2 Maintenance
Maintenance inspection checklist
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Chapter 3
Checking the Logs
Chapter 3 Checking the Logs 3.1
Logs and how to use them
You can check the following logs saved on the instrument. • Operation log: Displays the log of operations. • Error log: Displays the log of errors that have occurred. • Maintenance log: Displays the log of performed maintenance. • Reagent replacement log: Displays the log of reagent replacement.
Note: A maximum of 5,000 entries are saved in the operation log and a maximum of 1,000 entries are saved in the other logs; when the maximum number of entries is exceeded, each new entry deletes the oldest entry.
3.1.1
Checking a log
1 Touch the [Log] icon on the [Menu] screen. The [Log Menu] screen appears.
3.1.2 Adding comments to a log
Information An entered comment cannot be deleted. If there is an existing comment, any new comments entered are appended after the previously entered comment.
1 Display the screen for the log that you want to add a comment to. (➤P.97 "3.1.1
Checking a log")
2 Select the log that you want to add a comment to. [Log Menu] screen
2 Touch the icon for the desired log. The screen that shows the touched log appears.
3 Check the log. For details, see the following. (➤P.100 "3.2
Log screen") e.g. [Operation Log] screen
3 Touch the [Comments] button on the toolbar. The software keyboard appears.
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4 Enter the comment. Up to 100 characters can be entered.
Note: The following characters cannot be used for comments.
|\^&
5 Touch [OK]. The comment is added to the selected log.
3.1.3 Outputting the log in CSV format (USB flash drive) The log list can be output as a CSV file to a USB flash drive.
1 Display the screen to save the log. (➤P.97 "3.1.1
Checking a log")
3 Select the [File] button on the toolbar, and then touch [CSV Output(Removable Media)]. Saving starts and the [Outputting] dialog box appears. Saving is complete when the [Outputting] dialog box closes. When you save a log, the log is saved in a designated folder.
Note: Save location: \[Model name^Instrument ID]\OUTPUT File name: • Operation log: [Model name^Instrument ID][Software version] [Date of saving_Time of saving][OperationLog].csv • Error log: [Model name^Instrument ID][Software version] [Date of saving_Time of saving][ErrorLog].csv • Maintenance log: [Model name^Instrument ID][Software version] [Date of saving_Time of saving][MaintenanceLog].csv • Reagent replacement log: [Model name^Instrument ID][Software version] [Date of saving_Time of saving] [ReagentReplacementLog].csv
2 Insert the USB flash drive. Insert the USB flash drive into an available port on the back of the instrument.
4 Remove the USB flash drive.
A USB flash drive with a password lock function cannot be used. USB ports
USB flash drive
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3.1.4
Checking the Logs
Outputting the log in CSV format (host computer)
The log list can be output as a CSV file to the host computer*. * Output is enabled only when the instrument is connected to the host computer.
1 Display the screen to save the log. (➤P.97 "3.1.1
Checking a log")
2 Select the [File] button on the toolbar, and then touch [CSV Output(Network Drive)]. Saving starts and the [Outputting] dialog box appears. Saving is complete when the [Outputting] dialog box closes. When you save a log, the log is saved in a designated folder.
Note: Save location: \[Model name^Instrument ID]\OUTPUT File name: • Operation log: [Model name^Instrument ID][Software version] [Date of saving_Time of saving][OperationLog].csv • Error log: [Model name^Instrument ID][Software version] [Date of saving_Time of saving][ErrorLog].csv • Maintenance log: [Model name^Instrument ID][Software version] [Date of saving_Time of saving][MaintenanceLog].csv • Reagent replacement log: [Model name^Instrument ID][Software version] [Date of saving_Time of saving] [ReagentReplacementLog].csv
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Checking the Logs
Log screen Toolbar
Detailed display button
Log list
e.g. [Operation Log] screen Toolbar
Displays the function buttons.
[Option]
Touch to display the submenu and set the method of displaying the log list. [▲]: Sorts the log list by date and time in ascending order. [▼]: Sorts the log list by date and time in descending order. In the default setting, the list is in descending order.
[Comments]
Touch to display the software keyboard and enter a comment. (➤P.97 "3.1.2 Adding comments to a log")
[File]
Touch to display the submenu and save the log list. (➤P.98 "3.1.3 Outputting the log in CSV format (USB flash drive)")
Log list
Displays the list of logs.
Detailed display button Touch to display detailed information of a log that is selected in the log list. This information is displayed below the log list. Touch again to hide the detailed information.
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Checking the Logs
[Operation Log] screen
Log list
Detailed information
[Operation Log] screen Log list
Displays the list of logs.
[Operation Date]
Displays the date and time at which the log data was registered.
[Logon User Name]
Displays the [Operator Name] that was logged in when the log data was registered.
[Operation Name]
Displays the name of the performed operation.
Detailed information
Displays the detailed information of a log that is selected in the log list.
[Detailed Information:]
Displays the details of the performed operation. The information that appear vary depending on the operation. For details, see the following. (➤P.101 "●Operation names and display conditions/detailed information")
[Comments:]
Displays the entered comment.
● Operation names and display conditions/detailed information An operation log is registered when the following operations are performed. The followings are operation names and display conditions/detailed information. Operation name
Display condition
Detailed information
[Logon]
When the user logs on.
None
[Logoff]
When the user logs off or when the instrument is shut down.
None
[Validate Analysis Results]
When an analysis result is validated or cleared. Validation is not logged if data was automatically validated.
• Sample number • Validation status • Analysis date and time
[Modify Sample No. for analysis results]
When the sample number is modified.
• Sample number (before the change after the change) • Analysis date and time
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Operation name
Display condition
Detailed information
[Delete analysis results]
When an analysis result is deleted. A deletion is not logged if data was automatically deleted because the maximum number of registered data was exceeded.
When the number of deletions is 10 or less: • Sample number • Analysis date and time When the number of deletions exceeds 10: Number of deletions
[Register QC Lot]
When the lot information of a control material is registered.
• • • •
[Change bottle]
When the bottle of control material is changed.
• Control material name • Lot number • Expiration date (before the change after the change)
[Modify QC Lot]
When the lot information of a control material is modified.
• Control material name • Lot number • Expiration date (before the change after the change) • Setting of [Set additional standards for internal quality control] (enabled/disabled)*1 (before the change → after the change)
[Modify QC Target and Limit]
When the setting of the target and limit values for quality control is changed.
• • • •
[Delete QC Lot]
When a QC lot is deleted.
• Control material name • Lot number
[Delete QC Plot]
When a QC chart plot is deleted manually.
When the number of deletions is 10 or less: • Sample number • Analysis date and time When the number of deletions exceeds 10: Number of deletions
Control material name Lot number Expiration date Setting of [Set additional standards for internal quality control] (enabled/disabled)*1
Control material name Lot number Variable target computation*2 Calculation method*2
[Modify Settings]
When the instrument settings are changed.
Setting name
[Restore Settings]
When the instrument settings are restored.
None
[Initialize Settings]
When the instrument settings are initialized.
None
*1 For details, see "Basic Operation". (➤Basic Operation "Chapter 2: 2.5 Registering the lot information manually") *2 Displayed only when the setting of [Set additional standards for internal quality control] is enabled.
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3.2.2
Checking the Logs
[Error Log] screen
Log list
Detailed information
[Error Log] screen Log list
Displays the list of logs.
[Date]
Displays the date and time at which the log data was registered.
[Logon User Name]
Displays the user name that was logged in when the log data was registered.
[Status]
Displays the status of the error that occurred. [Error]: Error [Normal]: Error cleared
[Error Message]
Displays the message of the error that occurred.
Detailed information
Displays the detailed information of a log that is selected in the log list.
[Error Message:]
Displays the message of the error that occurred.
[Error Code:]
Displays the error code of the error that occurred.
[Error Level:]
Displays the level of the error that occurred.
[Error Parameter 1:]/ [Error Parameter 2:]
Displays the parameter 1 and parameter 2 of the error that occurred. Depending on the type of error, this field may be blank.
[Comments:]
Displays the entered comment. If the following errors occur, the rack ID and the tube position of the sample in which the error occurred are displayed in [Rack No.-Tube Pos.] (Example: [000001-01]). • [Positive ID Check Error] • [Failed to read sample number]
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3.2.3 [Maintenance Log] screen
Log list
Detailed information
[Maintenance Log] screen Log list
Displays the list of logs.
[Operation Date]
Displays the date and time at which the log data was registered.
[Logon User Name]
Displays the user name that was logged in when the log data was registered.
[Maintenance Items Name]
Displays the name of the maintenance task executed.
Detailed information [Comments:]
Displays the detailed information of a log that is selected in the list. Displays the entered comment.
● Maintenance tasks and display conditions A maintenance log is registered when the following maintenance tasks are performed. The following are the maintenance tasks and display conditions. Maintenance item
Display condition
[Cleaning]
When cleaning is performed.
[Rinse Flowcell]
When flowcell is rinsed.
[Background Check]
When a background check is performed.
[Sampler]*
When an operation test of the sampler is performed.
[Barcode Reader]*
When an operation test of the barcode reader is performed.
[Sheath Syringe]
When an operation test of the sheath syringe is performed.
[Charging Pump]
When an operation test of the charging pump is performed.
[Urine Pump]
When an operation test of the urine pump is performed.
[Dispensing Pump]
When an operation test of the dispensing pump is performed.
[Sample Arm]
When an operation test of the sample arm is performed.
[Dispensing Arm]
When an operation test of the dispensing arm is performed.
[Sample Filter]
When the sample filter is moved to the replacement position.
[Shutdown]
When shutdown is performed.
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Maintenance item
Checking the Logs
Display condition
[Restart] When restart is performed. * Maintenance can be performed only when the sampler (SA-51) is connected.
3.2.4
[Reagent Replacement Log] screen
Log list
Detailed information
[Reagent Replacement Log] screen Log list
Displays the list of logs.
[Operation Date]
Displays the date and time at which the log data was registered.
[Logon User Name]
Displays the user name that was logged in when the log data was registered.
[Reagent Name]
Displays the name of the replaced reagent.
[Lot]
Displays the lot number of the replaced reagent.
[Exp. Date]
Displays the expiration date of the replaced reagent.
Detailed information
Displays the detailed information of a log that is selected in the list.
[Serial No.:]
Displays the serial number within the lot of the replaced reagent.
[Shelf life after opening:]
Displays the shelf life of the replaced reagent after it has been opened.
[Exp. Date:]*
Displays the expiration date of the replaced reagent.
[Volume:]
Displays the amount of replaced reagent for diluent and sheath fluid. Displays the number of tests for the replaced reagent for dye.
[Entry Type:]
Displays the method used to enter the replaced reagent.
[Product Code:]
Displays the entered product code.
[Manufacturer:]
Displays the entered manufacture.
[Address:]
Displays the entered manufacturer address.
[Comments:]
Displays the entered comment.
* [Exp. Date:] is the opening date + shelf life after first opening, or the expiration date for the unopened product, whichever is earlier.
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Index
Index A Adjusting the 0.05 MPa pressure.......................15 Adjusting the 0.24 MPa pressure.......................14 Adjusting the vacuum.........................................19
B Background check .............................................68
C Causes of errors and corrective actions ............13 Analysis .........................................................41 Barcode .........................................................39 Covers ...........................................................43 Dye holder .....................................................44 Fan ................................................................33 Hydraulic system ...........................................23 Laser..............................................................33 Mixing/aspiration/analysis operation..............32 Other..............................................................49 Pressure ........................................................13 Quality control................................................40 Reagent and rinse water ...............................25 Replacement parts ........................................43 Sample aspiration..........................................34 Sample holder ...............................................39 Sampler analysis ...........................................47 System...........................................................40 Temperature control ......................................21 Waste fluid.....................................................31 Checking the status of instrument......................65 Pressure/temperature/laser current...............65 Sensor (sampler) ...........................................66 SV/sensor (analyzer) .....................................65 Cleaning................................................34, 42, 69 Counter ..............................................................67
D Disposing of waste fluid in the waste tank ...................................... 31, 81 Dye holder..........................................................44
E Error log ...........................................................103 Error message list (in alphabetical order) ............7 [ErrorHelp] dialog box ........................................12 Errors related to analysis ...................................41 Errors related to barcode ...................................39 Errors related to covers......................................43 Errors related to fan ...........................................33 Errors related to hydraulic system .....................23 Errors related to laser ........................................33
Errors related to mixing/aspiration/analysis .............................. 32 Errors related to pressure ................................. 13 Errors related to quality control ......................... 40 Errors related to reagent and rinse water.......... 25 Errors related to replacement parts................... 43 Errors related to sample aspiration ................... 34 Errors related to sample holder......................... 39 Errors related to sampler analysis operation ............................ 47 Errors related to system .................................... 40 Errors related to temperature control ................ 21 Errors related to waste fluid .............................. 31
F Fuse replacement ............................................. 91 Instrument..................................................... 91 Pneumatic unit .............................................. 93
L Logs .................................................................. 97 Checking the logs ......................................... 97 Comments .................................................... 97 CSV output (host computer) ......................... 99 CSV output (USB flash drive) ....................... 98 [Error Log] screen ....................................... 103 [Maintenance Log] screen .......................... 104 [Operation Log] screen ............................... 101 [Reagent Replacement Log] screen ........... 105
M Maintenance...................................................... 63 Task list......................................................... 63 Maintenance inspection checklist...................... 95 Maintenance log.............................................. 104 [Maintenance Menu] screen.............................. 64
O Operation log................................................... 101 Operation tests.................................................. 72 Arm ............................................................... 76 Barcode reader ............................................. 74 Menu screen ................................................. 72 Pump ............................................................ 76 Sampler ........................................................ 72 Syringe.......................................................... 75
P Pressure adjustment 0.05 MPa pressure ....................................... 15 0.24 MPa pressure ....................................... 14 Vacuum......................................................... 19
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R Reagent replacement........................................ 82 Dialog box..................................................... 82 Diluent and sheath fluid .......................... 25, 83 Dye ...................................................28, 45, 85 Reagent replacement log ................................ 105 Remedial actions against U-RDU failure........... 50 Replenishing Rinse water................................................... 30 Rinse water Cleaning the bottle........................................ 78 Replenishing ........................................... 30, 77 Rinsing the flowcell ........................................... 70 Rinsing/replacing the sample filter .............. 35, 87
S [Status Disp.] dialog box ................................... 65
T Trap chamber Checking the volume of fluid and removing fluid ..................... 17, 79
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