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CELL-DYN Emerald System Service and Support Manual



CELL-DYN Emerald System Service and Support Manual



Manual Revision Number 202661-106 Front Matter Document Control Number 202662-106 © 2007, 2016 Abbott Laboratories, Abbott Park, IL. All rights reserved.



Revision Log The Revision Status of the manual is indicated below. Be sure the manual contains the latest revision number of all pages. Click for Chapter 4 Removal and Replacement and Chapter 5 Verification Procedure Revision History All other Revision History is located in the table below: Revision



Date



Sections Revised/added



TSBs Incorporated ISAs Incorporated



Revision 202661-106 Change Listing N/A



N/A



202661-105 November 2010 Revision 202661-105 Change Listing N/A



N/A



202661-104 October 2009



Revision 202661-104 Change Listing N/A



N/A



202661-103 December 2008 Revision 202661-103 Change Listing N/A



N/A



202661-106 February 2016



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3H_titl.htm[4/4/2016 10:17:41 AM]



CELL-DYN Emerald System Service and Support Manual



202661-102 March 2008



Revision 202661-102 Change Listing N/A



202661-101 November 2007 NEW



N/A



N/A N/A



CELL-DYN Emerald Service and Support Manual (Version 202661-106) • © 2007, 2016 • CELL-DYN, CELL-DYN Emerald and CELL-DYN Emerald 22 are trademarks of Abbott Laboratories in various jurisdictions. • Abbott Park, IL 60064 USA • All rights reserved.



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3H_titl.htm[4/4/2016 10:17:41 AM]



Proprietary Information (Document Control Number 204681-102) The information, documents and related graphics published herein (the "Information") are the sole property of Abbott Laboratories. Permission to use the Information is granted, provided that the copyright notice appears on all copies; use of the Information is for operation of ABBOTT products by Abbott trained personnel or informational use only; the Information is not modified in any way; and no graphics are used separate from accompanying text. Qualifications: All samples (printouts, graphics, displays, screens, etc.) are for information and illustration purposes only and shall not be used for clinical or maintenance evaluations. Data shown in sample printouts and screens do not reflect actual patient names or test results. The information was developed to be used by Abbott Laboratories-trained personnel, by other persons knowledgeable or experienced with the operation and service of the product identified, under the supervision and with cooperation from Abbott Laboratories technical support or service representatives. In no event shall Abbott Laboratories or its affiliates be liable for any damages or losses incurred in connection with or arising from the use of the information by persons not fully trained by Abbott Laboratories. This limitation shall not apply to those persons knowledgeable or experienced with the operation and service of the product identified, under the supervision and with cooperation from Abbott Laboratories technical sales or service representatives. No part of this media may be reproduced, stored, retrieved, or transmitted in any form or by any means without the prior written permission of Abbott Laboratories. No confidential relationship shall be established in the event that any user of the Information should make any oral, written or electronic response to Abbott Laboratories (such as feedback, questions, comments, suggestions, ideas, etc.). Such response and any information submitted therewith shall be considered non-confidential, and Abbott shall be free to reproduce, publish or otherwise use such information for any purposes whatsoever including, without limitation, the research, development, manufacture, service, use, or sale of products incorporating such information. The sender of any information to Abbott is fully responsible for its content, including its truthfulness and accuracy and its non-infringement of any other person's proprietary rights. Abbott Laboratories is not engaged in rendering medical advice or services. Updates to the Information may be provided in either paper or electronic format. Always refer to the latest documents for the most current information. All Abbott Laboratories product names and trademarks are owned by or licensed to Abbott Laboratories, its subsidiaries or affiliates. No use of any Abbott trademark, trade name, trade dress, or product name may be made without the prior written authorization of Abbott Laboratories, except to identify the product or services of Abbott Laboratories. All other trademarks, brands, product names, and trade names are the property of their respective companies. All rights reserved. Except as permitted above, no license or right, express or implied, is granted to any person under any patent, trademark, or other proprietary right of Abbott Laboratories. ABBOTT LABORATORIES MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND OR NATURE WITH RESPECT TO THE INFORMATION. ABBOTT LABORATORIES HEREBY DISCLAIMS ALL REPRESENTATIONS AND WARRANTIES, WHETHER EXPRESS OR IMPLIED, CREATED BY LAW, CONTRACT OR OTHERWISE, INCLUDING, WITHOUT LIMITATION, ANY WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, TITLE OR NON-INFRINGEMENT. IN NO EVENT SHALL ABBOTT LABORATORIES BE LIABLE FOR ANY DAMAGES OF ANY KIND OR NATURE, INCLUDING, WITHOUT LIMITATION, DIRECT, INDIRECT, SPECIAL (INCLUDING LOSS OF PROFIT) CONSEQUENTIAL OR INCIDENTAL DAMAGES ARISING FROM OR IN CONNECTION WITH THE EXISTENCE OR USE OF THE INFORMATION, REGARDLESS OF WHETHER ABBOTT LABORATORIES HAS BEEN ADVISED AS TO THE POSSIBILITY OF SUCH DAMAGES. CELL-DYN and CELL-DYN Emerald are trademarks of Abbott Laboratories in various jurisdictions.



CELL-DYN Emerald Service and Support Manual (Version 202661-105) • © 2007, 2010 • CELL-DYN and CELL-DYN Emerald are trademarks of Abbott Laboratories in various jurisdictions. • Abbott Park, IL 60064 USA • All rights reserved.



Revision 202661-102 Change Listing This page lists the changes from 202661-101 to Revision 202661-102.



Section Sections Numbers Revised/Added



Revision



Front



Title



Manual Revision Number; change to "202661-102"



Front



Title



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Update publication date and Revision Status for 202661-102



Front



Prop Info



Change Control Number 204681-101.



Chap 4



Title



Document Control Number, change to "202666-102"



Chap 4



Locator table



Parts and RR names will be pulled directly from approved records in FRU.



Chap 4



All RR procedures



Parts and verification information will be pulled directly from approved records in FRU. A note dynamically sourced from FRU, "Inspect tools for damage, ensure calibration is not expired and replace if necessary.", has been added at the beginning of the RR. An additional verification procedure, G110, has been added as the final verification of each RR.



Chap 5



Sect title pg



Change to "Document Control Number 202667-102".



Chap 5



All VPs



Two notes dynamically sourced from FRU, "Inspect tools for damage, ensure calibration is not expired and replace if necessary." and "At the completion of this VP:  After repair is complete, verify per released Operation and Service Procedures. If the system/instrument produces results, ENSURE appropriate Quality Control is in specification and calibrate as necessary.", have been added at the beginning of the procedure.



CELL-DYN Emerald Service and Support Manual (Version 202661-102) • © 2007, 2008 • CELL-DYN is a registered trademark of Abbott Laboratories. Emerald is a trademark of Abbott Laboratories. • Abbott Park, IL 60064 • All rights reserved.



Revision 202661-103 Change Listing This page lists the changes from 202661-102 to Revision 202661-103.



Section Numbers



Sections Revised/Added



Revision



Front



Title



Manual Revision Number; change to 202661-103.



 



 



Front Matter Document Control Number; change to 202662-103.



Front



Rev Status



Update Revision Status for 202661-103.



 



 



 



Chap 1



General Data



Document Control Number, change to 202663-102.



 



 



Added Radio Frequency Hazard.



 



 



Updated Fluidics Diagram graphic 3H_9005b.



 



 



Changed “Needle” to “Probe” global update in entire service manual.



 



 



Under User Access Menus deleted graphics 3H_6004, 3H_6005, and 3H_6006 and replaced with 3H_6009.



 



 



Service Password changed SERVICE to MAINTENANCE as a Global update in entire service manual.



 



 



Service Screen rearranged order of bullet points and deleted one bullet point.



 



 



Bar Code Reader, Pin Functions deleted a graphic 3H_1001 and replaced it with a table.



 



 



 



Chap 2



Troubleshooting



Document Control Number, change to 202664-102.



 



 



Update Wiring Diagram – General View 3H_9003b.



 



 



 



Chap 5



Verification Procedures



Updated Verification Procedure Table adding new VP.



CELL-DYN Emerald Service and Support Manual (Version 202661-103) • © 2007, 2008 • CELL-DYN is a registered trademark of Abbott Laboratories. Emerald is a trademark of Abbott Laboratories. • Abbott Park, IL 60064 • All rights reserved.



Revision 202661-104 Change Listing This page lists the changes from 202661-103 to Revision 202661-104.



Section Numbers



Sections Revised/Added



Front



Title



Manual Revision Number; change to 202661-104.



 



 



Front Matter Document Control Number; change to 202662-104.



Front



Rev Status



Update Revision Status for 202661-104.



 



 



 



Chap 1



General Data



Document Control Number, change to 202663-103.



 



 



Update 3H_6009b..



Revision



CELL-DYN Emerald Service and Support Manual (Version 202661-104) • © 2007, 2009 • CELL-DYN is a registered trademark of Abbott Laboratories. Emerald is a trademark of Abbott Laboratories. • Abbott Park, IL 60064 • All rights reserved.



Revision 202661-105 Change Listing This page lists the changes from 202661-104 to Revision 202661-105.



Sections Revised/Added



Section Numbers



Revision



Front



Title



Manual Revision Number; change to 202661-105.



 



 



Front Matter Document Control Number; change to 202662-105.



Front



Proprietary Information Change Control Number 204681-102.



 



 



Updated trademark information.



 



 



 



Chap 1



General Data



Document Control Number, change to 202663-104.



 



 



Updated dilution rate in Fluidics and added a graphic 3H_9023a.



 



 



Added new text to Counting Module area.



CELL-DYN Emerald Service and Support Manual (Version 202661-105) • © 2007, 2010 • CELL-DYN and CELL-DYN Emerald are trademarks of Abbott Laboratories in various jurisdictions. • Abbott Park, IL 60064 USA • All rights reserved.



Revision 202661-105 Change Listing



Revision 202661-106 Change Listing This page lists the changes from 202661-105 to Revision 202661-106.



Section Numbers Front



Sections Revised/Added



Revision



Title



Manual Revision Number; change to 202661-106.



Front Matter



Document Control Number; change to 202662-106.



 



 



 



Chapter 1



General Data



Document Control Number; change to 202663-105.



 



 



Update Electrical System, Fluidics, Instrument Alarms, Operational Alerts, Data Flags and Software with Emerald 22 content.



 



 



 



Chapter 2



Troubleshooting Document Control Number; change to 202664-103.



 



 



Add CELL-DYN Emerald 22 Drawing/Schematics and links.



 



 



Add CELL-DYN Emerald 22 Troubleshooting.



 



 



 



Chapter 6



Maintenance



Document Control Number; change to 202668-102.



 



 



Add CELL-DYN Emerald 22 Preventive Maintenance checklist and ISA link.



 



 



 



Chapter 8



Installation



Add CELL-DYN Emerald 22 Installation, Integration & Preventive Maintenance checklist and links.



 



 



 



Chapter 9



Printer Compatibility



Add Active Printer Compatibility & Validated document control 201729-113.



CELL-DYN Emerald Service and Support Manual (Version 202661-106) • © 2007, 2016 • CELL-DYN, CELL-DYN Emerald and CELL-DYN Emerald 22 are trademarks of Abbott Laboratories in various jurisdictions. • Abbott Park, IL 60064 USA • All rights reserved.



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General Data (Document Control Number 202663-105)



CELL-DYN Emerald Service and Support Manual (Version 202661-106) • © 2007, 2016 • CELL-DYN, CELL-DYN Emerald and CELL-DYN Emerald 22 are trademarks of Abbott Laboratories in various jurisdictions. • Abbott Park, IL 60064 USA • All rights reserved.



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How to Use This Manual



Links



Biological Hazards Chemical Hazards Electrical Safety Electrostatic Discharge (ESD) Hazards Overview Physical Hazards Safety Icons and Hazard Symbols



Note Before installing or maintaining the CELL-DYN Emerald, read the CELL-DYN Emerald System Operations Manual. Overview The purpose of this manual is to provide information useful for servicing the CELL-DYN Emerald system and is composed of the following sections:



Section



Topic



General Data



This section contains a product overview, information on manual usage, accident prevention symbols, and system specifications.



Troubleshooting



This section contains reference and normal operation information for the system.



Removal & Replacement



This section contains Removal & Replacement procedures which are indexed by number to the FRU (Field Replaceable Unit) database.



Procedures



This section contains configuration, adjustment, calibration, checks, and test procedures used to setup or verify instrument operation. Procedures are also used to assist in troubleshooting.



Planned Maintenance



This section contains preventative maintenance (PM) recommendation checklists. These recommendations may be subject to change due to local business or regulatory needs.



Pre-Site Specification & Checklist



This section includes the site specifications and a checklist that outlines the activities of the Field Service Engineer/ Representative (FSE/FSR). An open action item summary is included in the checklist.



Installation



This section outlines the activities required to install or relocate a system.



Hazards



Introduction Operation, maintenance and servicing of CELL-DYN Emerald system may expose individuals to potential safety and health hazards. All work must be performed in accordance with procedures described in the Abbott operations or service manuals. This section describes the types and locations of potential hazards that could cause physical harm to service personnel. Warnings are inserted throughout this manual to alert service personnel to potential hazards. Standard warning conventions including hazard signal words (example, Danger) and symbols are described below. Definitions Hazard signal word definitions are described below.



Signal Word



Definition



DANGER Denotes an immediate hazard which, if not avoided, could result in serious injury or death. This signal word represents the highest level of any hazardous situation.



WARNING



Denotes a hazard which could result in moderate to serious personal injury.



Caution



Denotes potential hazards that could result in minor injury. Also used for conditions or activities that could interfere with proper functioning or performance of the instrument.



Note



Denotes operator or service information.



Safety Icons and Hazard Symbols Safety hazard symbols are used in this manual and on instrument labels to identify potentially dangerous conditions or situations. In this manual and on some instrument labels, text accompanies the safety symbol to describe the hazard, or symbols may be used in lieu of text. For other instrument labels, the operator is to refer to the manual for specific information, or you must recognize the symbols and understand the type and degree of potential hazard.



Symbol



Hazard



Description



WARNING Electrical Shock Hazard



Identifies the possibility of electrical shock if procedural or engineering controls are not observed.



WARNING Potential Biohazard



Identifies an activity or area where potentially infectious materials may be present. Follow procedures as outlined below in Biological Hazards Section.



Caution Chemical Hazard



Identifies an activity or an area where hazardous chemicals are present. Refer to the Material Safety Data Sheet (MSDS) or package insert for specific safety information.



Caution Radio Frequency



Do not use mobile telephones, wireless telephones, mobile radios, or any other radio frequency (RF) transmitting devices in the same room as the instrument.



Hazard Caution Identifies an area where electrostatic discharge may be present. A ground strap must be worn Electrostatic while servicing the system. Discharge



The labeling of CELL-DYN Emerald system reagents/calibrators/controls or liquid consumables may include one or more of the following hazard symbols. The symbols and/or other country specific warnings are used to convey properties of the chemical or chemical mixture, and to notify the user that precautions should be taken when handling the material. Always consult the specific package insert or Material Safety Data Sheet for further information.



Hazard Symbol Definition / Description (with Standard Abbreviation) Indicates that the material is Harmful (Xn) or Irritant (Xi).



Electrical Safety The CELL-DYN Emerald system does not pose uncommon electrical hazards to operators if it is installed and operated without alteration, and is connected to a power source that meets required specifications. Basic electrical hazard awareness is essential to the safe operation of any system. Only qualified field service personnel should perform electrical servicing. Elements of electrical safety include, but are not limited to the following: Periodically inspect electrical cabling into and on the system for signs of wear and damage. Turn the instrument OFF before disconnecting the power cord and before cleaning, servicing, or performing maintenance on any electrical or internal component. In the event of a blown fuse or thrown circuit breaker, determine the cause and correct the problem before attempting to resume operation of the equipment. Replace only the fuses that are externally accessible and labeled. Only use replacement fuses of the specified type and electrical rating. Assure the power to the instrument is turned OFF. A high voltage charge may remain on the power supply with the power OFF. Use an electrically insulated tool to disconnect the power supply and short (both male pins) to the instrument chassis. Keep liquids away from all connectors of electrical or communication components. Unplug the instrument before clean-up of major liquid spills. Clean spilled fluids immediately. Do not touch any switches or outlets with wet hands. Keep the floor dry and clean under and around the system. Use only approved power cords and electrical accessories, such as those supplied with the instrument, to protect against electric shock. Connect power cords only to properly grounded outlets. It is recommended that a ground fault circuit interrupter be used when working in a wet environment. Mechanical Hazards The CELL-DYN Emerald system is an automated system that operates under computer control. As with most automated equipment, there is potential for injury and bodily harm from moving mechanical components whenever the instrument is in operation. The system minimizes mechanical hazards by providing guards to protect against accidental contact with moving components, and encoding the software with safety features.



Operators of the CELL-DYN Emerald system are potentially exposed to moving mechanical components such as the syringe panel. Use caution when performing any maintenance procedure on the syringe panel as moving parts can pinch. Basic elements of mechanical equipment safety include but are not limited to: Never bypass or override a safety device. Keep all protective covers and barriers in place. Never perform manual tasks on the work surface of the System. Never allow any part of your body to enter a range of mechanical movement during System operation. Do not wear articles of clothing or accessories that could catch on the System. Keep pockets free of items that could fall into the System. Be especially cautious when performing adjustment, maintenance, cleaning, or repair procedures. Use caution when loading reagents. In the event of an instrument malfunction or an unexpected sequence of movements, be aware that unexpected field service personnel reflex actions could occur, causing injury. Biological Hazards The following activities may involve the presence of biological materials: Handling samples, reagents, calibrators, and controls Cleaning spills Handling and disposing of waste Moving the System Performing maintenance procedures Performing decontamination procedures Performing component replacement procedures Precautions Consider all clinical specimens, reagents, controls, and calibrators that contain human sourced material and instrument surfaces or components that have come in contact with human sourced material as potentially infectious. No known test method can offer complete assurance that products derived from human sourced material or instrument components exposed to human sourced material will not transmit infection. Therefore, all products derived from human sourced materials and instrument components exposed to human sourced material should be considered potentially infectious. It is recommended that all potentially infectious materials be handled in accordance with the OSHA Bloodborne Pathogens Rule (29 CFR Part 1910.1030) or other equivalent biosafety procedures. Precautions include, but are not limited to the following: Wear gloves, lab coats, and protective eye wear when handling human sourced material or contaminated instrument components. Do not pipet by mouth. Do not eat, drink, smoke, apply cosmetics, or handle contact lenses when handling human sourced material or contaminated instrument components. Clean spills of potentially infectious materials and contaminated instrument components with an appropriate tuberculocidal disinfectant, such as 0.5% sodium hypochlorite or other suitable disinfectant. Decontaminate and dispose of all specimens, reagents, and other potentially contaminated materials in accordance with local, state, and federal regulations. If you are exposed to biohazardous or potentially infectious materials you should seek medical attention immediately and take the following steps to cleanse the affected area: Eyes-rinse with water for 15 minutes. Mouth-rinse with water. Skin-wash the affected area with soap and water. Puncture wound-allow to bleed freely. Wash the affected area with soap and water.



Sharps Probes, needles, aspiration probes are sharp and potentially contaminated with infectious materials. Avoid contact with the tips of these parts. Handling Spills Clean spills in accordance with established biosafety practices. In general, safe work practices for cleaning spills include:



1. 2. 3. 4. 5.



Wear appropriate personal protective equipment. Absorb the spill with absorbent material. Wipe the spill area with detergent solution. Wipe the area with an appropriate tuberculocidal disinfectant such as a 0.5% sodium hypochlorite solution. Dispose of spilled and contaminated material in accordance with local, state, and federal regulations.



Instrument or Part Decontamination Always wear appropriate personal protective equipment (protective eye wear, gloves, lab coat) while performing decontamination activities. Prior to service or maintenance, the instrument should be decontaminated in accordance with the following:



1. Remove and dispose of contaminated disposables in a regulated medical waste container. 2. Clean and decontaminate the exterior of the instrument using a detergent solution followed by a 0.5% sodium hypochlorite



solution or other tuberculocidal disinfectant. Flush the fluid pathway as specified in the CELL-DYN Emerald System Operations Manual. For information on preparing the proper concentration of sodium hypochlorite solution, refer to Appendix C in CELL-DYN Emerald Operator's Manual. Caution Under normal circumstances, printed circuit boards do not require decontamination. Field Replaceable Units (FRUs) enclosed inside the skins of computer and peripheral equipment are not considered to be contaminated. Decontamination may affect the performance of a printed circuit board or internal computer component.



Handling Waste Dispose of all potentially infectious materials (clinical specimens, reagents, controls, calibrators, standards, cuvettes, liquid consumables, and contaminated gloves, wipes, swabs, and other disposables that may be contaminated) in accordance with local, state, and federal regulations. Sharps, such as probes, needles, broken glass, slides and other sharps that are contaminated with potentially infectious substances, should be placed in an appropriately labeled, puncture resistant and leak proof container before treatment and disposal. Chemical Hazards You may be exposed to hazardous chemicals when handling reagents, calibrators, controls, or liquid consumables. Exposure to hazardous chemicals is minimized by following instructions provided in the assay-specific Package Inserts and Material Safety Data Sheets (MSDS). Exposure levels are further reduced by the design features of the instrument when it is used properly. Precautions In general, observe the following precautions when handling chemicals: Consult Material Safety Data Sheets for safe use instructions and precautions. Avoid contact with skin and eyes. If contact with material is anticipated, wear impervious gloves and protective eye wear and clothing. Always maintain good housekeeping. Do not eat, drink, or store food and beverages in areas where chemicals are used. If irritation or signs of toxicity occur after exposure, seek medical attention. Hazard symbols that display on CELL-DYN Emerald system product labeling are accompanied by Risk (R) and Safety (S) numbers



and represent specific risk and safety phrases as defined by European Community Directives. The risk and safety phrases describe precautions to be used when working with a particular chemical or chemical mixture. For all (R) and (S) numbers that display on product labeling, refer to the corresponding phrases indicated in the Package Insert or similar document. Physical Hazards Sharps and Probes The probe, vent needle, and aspiration probe are sharp and potentially contaminated with infectious materials. Avoid contact and handle cautiously to prevent injury. Never reach into the instrument while it is in operation. In general, use of sharps and glassware should be minimized. Use mechanical means to remove contaminated broken glassware. Dispose of sharps in an appropriately marked, puncture-resistant, and leakproof container before treatment and disposal. Heavy Objects The waste container is heavy when full. Use care when handling the container to reduce the risk of injury. The system is heavy and has unsupported sections of the shell. Ensure that you have adequate help before attempting to move the system. Push only on solid sections of the housing; do not exert pressure on unsupported sections of the shell. Use proper lifting techniques when moving the System. Trip Hazard The System is equipped with a power cord and various computer connectors. To avoid a tripping hazard, ensure cords in high traffic areas are properly stowed. Radio Frequency Hazard Do not use mobile telephones, wireless telephones, mobile radios, or any other radio frequency (RF) transmitting devices in the same room as the instrument. Electrostatic Discharge (ESD) Many of the electronic components on the System circuit boards are susceptible to electrostatic discharge (ESD). Static discharge of as little as 100 - 200 volts can damage or destroy a component. To put that in perspective, depending on the floor covering, relative humidity and other factors, walking across a floor generates between 250 - 35,000 electrostatic volts. Attempts to ground oneself and remove the static charge by grasping the instrument chassis provides only momentary resolution. Static Hazard Static protective procedures are used during the manufacture of PC boards. Replacement PC board assemblies are also protected by use of static protective packaging as well as boxed to prevent physical damage. Assemblies that have failed and are returned for repair are also handled at the repair shop under static protection procedures. Handling Guidelines - PC Subassemblies These guidelines assure protection against failures created by static. Retain spare PC board subassemblies in the static-protective bags. Use an approved static-protective field service kit, or the ground strap shipped with the board, whenever a board is removed from an instrument or protective bag. Replace the defective PC board in the same protective bag to return for repair. Continued use of the protective shipping boxes, both during shipping and storage, eliminates most failures caused by physical damage.



ESD Procedure Note Use where ESD symbol is present and static protective equipment is not shipped with replacement part.



1. Place the work mat on a solid surface close to the instrument, allow the ground strap to reach the instrument. 2. Attach the ground clip to the instrument chassis. 3. Attach the other end of the ground clip cable and the connector from the wrist strap to the work mat. (Exception: Some wrist 4. 5. 6. 7.



strap cables provide a clip to connect to the same ground source as the mat cable). Attach wrist strap to your wrist, make sure the metallic button on the inside of the wrist strap is in direct contact with your skin. Place PC boards, removed from the instrument, on the work mat. Replacement PC boards should be placed on the work mat before removing from the protective bag and remain on the mat until installation. Defective PC boards should be replaced in the static-protective bag before removal from the work mat area.



CELL-DYN Emerald Service and Support Manual (Version 202661-103) • © 2007, 2008 • CELL-DYN is a registered trademark of Abbott Laboratories. Emerald is a trademark of Abbott Laboratories. • Abbott Park, IL 60064 • All rights reserved.



Bar Code Reader The CELL-DYN Emerald accepts any brand of bar code reader that conforms to the following specifications. RS232 Specification Pin Functions On the Emerald side: DB9 male: Pin



Description



(2) RXD Receives data from scanner to CELL-DYN Emerald (mandatory). (3) TXD Transmitted data from CELL-DYN Emerald to scanner (optional). (5) GND Signal ground (mandatory). (7) CTS Clear to send (output). (8) RTS Request to send (input). (9)



+5V



RS232 Settings The bar code reader should be set to: Baud Rate: 9600 Data: 8 bits Parity: none Stop bits: 1 Code Options The CELL-DYN Emerald is able to read UPCA, EAN 13, EAN 128, Code 39, Code 93, Code 128, Codabar, Industrial 20f5, Interleaved 20f5, UPC E, Chinese Post, EAN 8, IATA, MARTRIX 20f5, ITALIAN Pharmaceutical, Telepen, and TRI Optic.



CELL-DYN Emerald Service and Support Manual (Version 202661-103) • © 2007, 2008 • CELL-DYN is a registered trademark of Abbott Laboratories. Emerald is a trademark of Abbott Laboratories. • Abbott Park, IL 60064 • All rights reserved.



Electrical System



Electrical System



Overview The content of this section applies to CELL DYN Emerald and CELL-DYN Emerald 22. The CELL-DYN Emerald and CELL-DYN Emerald 22 consist of three electronic boards: Main CPU board Preamplifier board IHM board Caution In the case of replacement of the main board or preamplifier board, be aware of the sensitivity of this board to the electrical static discharge (ESD). Caution Before operating check the earth connection and use the antistatic bag supply with the board. Caution Be careful with the edge of the metal sheets after having dismantled the covers. Electric Parts View CELL-DYN Emerald CPU Board:



CELL-DYN Emerald 22 CPU Board:



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Electrical System



The Main CPU board is located between the hydraulic part and the reagent tray. The board, driven by a 32-bit processor, manages the following parts: Sample needle, rocker, syringe block motors Display and keyboard Connection mode (RS232, Ethernet, WB) Printer Measurement (Counting, hemoglobin measurement and optical) Data processing External barcode reader Two fuses, which protect the heater circuit Pre-Amplifier Board and Cover



CELL-DYN Emerald IHM Printed Circuit Board



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Electrical System



CELL-DYN Emerald 22 IHM Printed Circuit Board



CELL-DYN Emerald Power Supply Assembly



CELL-DYN Emerald 22 Power Supply Assembly



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Electrical System



  CELL-DYN Emerald is supplied with an external power supply block. If the main power wire supplied with the CELL-DYN Emerald is replaced, the new one must comply with the local regulation. The CELL-DYN Emerald has been certified with the power supply box provided with the instrument. The use of another external power supply box is not guaranteed.



CELL-DYN Emerald Wiring Diagram General View



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Electrical System



CELL-DYN Emerald 22 Wiring Diagram



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Electrical System



CELL-DYN Emerald Service and Support Manual (Version 202661-106) • © 2007, 2016 • CELL-DYN, CELL-DYN Emerald and CELL-DYN Emerald 22 are trademarks of Abbott Laboratories in various jurisdictions. • Abbott Park, IL 60064 USA • All rights reserved.



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Fluidics



Fluidics GENERALITIES A volume of insufficient blood for the quantity of anticoagulant or a bad mixing may involve an erroneous result.



Two dilution rates are performed: 1/170 for WBC/HGB 1/10,000 for RBC/PLT Emerald 22 fluidics is based on stepping motors, and therefore, precise volumes are easily controlled by a number of steps.



FLUIDICS SEQUENCES DESCRIPTION Note Every time the counting chamber is drained (valve 1 or 2), we check for negative pressure (vacuum) to determine if there is an obstruction. We're looking for atmospheric pressure.



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Fluidics



Sampling Sequence



1. Syringes down: (no valve)



2. 3. 4. 5. 6. 7. 8.



Approximately 16.7 µl of blood sample is aspirated into the sampling probe. Diluent and lyse fill from the reagent bottles. A vacuum is created. Probe up and syringe up (valve 3+4) open before syringe movement Outside of probe cleaned with diluent (v4) (-0,7ml) and waste vacuum (v3) Rocker moves above the WBC bath (v3 v4 close), and the syringes move up to drain the waste (valve 7). Probe moves down to the rinsing position (valves closed). Syringe down WBC bath drain (valve 1) Tubing transfer (optical staging tubing) drain (valve 11 + 14) Syringe up Second outside needle cleaning (~1,5ml) (valve 4) Waste syringe drain (valve 7) Syringe down WBC bath drain (valve 1) Syringe up Waste syringe drain (valve 7) Dilutions



WBC Dilution



9. Needle down to the dilution position, and the syringes down (Valve 8 and 18 open to equilibrate to atmospheric pressure.) https://gssweb.abbott.com/WWCS/hematology/emerald_html/3103.htm[4/4/2016 10:20:10 AM]



Fluidics



10. Syringe up for the WBC dilution (~2 mL) Diluent outside of the needle (valve 4) 0.5 mL Sample/Diluent inside the needle (valve 4+5) 1.5 mL Bubble to mix the dilution (valve 1)



RBC Dilution and WBC Lysing



11. Syringe down (no valve)



12. 13. 14.



15. 16. 17.



Drawing sample of the first dilution (~25µl) Diluent syringe fill (See step 1.) Vacuum creation Needle up and syringe up Rocker move above the RBC bath Needle outside cleaning with diluent (~0,5mL) and waste vacuum (valve 3+4) Needle down Syringe up Second outside needle cleaning (~0,9mL) (valve 4+8) Lysis in the WBC bath (0,73mL) (valve 9) Bubble to mix the WBC dilution (valve 1) Syringe down RBC bath drain (valve 2) Needle down to the dilution position Syringe up for the RBC dilution (~2mL) Waste syringe drain (valve 7) Diluent outside of the needle (valve 4) 0.5 mL Sample dilution and Diluent inside the needle (valve 5) 1.5 mL Bubble to mix the dilution and diluent to backflush the counting heads prior to measurement (valve 6+10+2)



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3103.htm[4/4/2016 10:20:10 AM]



Fluidics



Measurements



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3103.htm[4/4/2016 10:20:10 AM]



Fluidics



18. Syringe down to create vacuum to process the counting (valve 8+10) and check the vacuum (negative pressure) and stage the sample for optics (valve 11+14) 19. First measurement sequences (first count), using vacuum created by syringes (2 second) (valve 10) 20. Syringe up Diluent back pressure (valve 4+6)



21. Syringe down to generate vacuum for second count (valve 8) 22. Second measurement sequences CBC measurements (time: 8 seconds), (v10) by the use of the vacuum in the vacuum tank At the same time, syringe up for DIF measurements, (time: 12 seconds) (valve 4+13 to push diluent), (9+12 and 17 to push lyse) (remaining sample through tubing 48) and 16 to waste



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3103.htm[4/4/2016 10:20:10 AM]



Fluidics



Note The optical and impedance counts start at the same time, w/optical finishing 4 seconds after impedance.



Drain and Rinse Bath



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3103.htm[4/4/2016 10:20:10 AM]



Fluidics



23. Cleaning 24.



25. 26. 27. 28.



Use residual vacuum from the end of step 22 to clean the apertures (valve 10 and v15) (w/ cleaner). At the end of the optics sequence the syringes move up a little more, creating back pressure in the optical injector (valve 4+13+14). Syringe down WBC bath drain (valve 1) Optical sample staging tubing drain (valve 11+14) RBC bath drain (valve 2) Syringe up RBC bath rinse diluent (~2mL, valve 4) Waste syringe drain (valve 7) Syringe up for aperture back flush (valve 4+6) Needle up (always when rocker moves). Rocker moves above the WBC bath. Syringe moving up Filling WBC bathw/ rinse diluent (~2,5mL, valve 4) Waste syringe drain (valve 7) Open v8 v18 to evacuate all vacuum.



NEEDLE BACK AND WASTE DRAIN



29. Syringe down 30. 31. 32.



Optical sample staging Transfer tubing rinsing with ~0,5mL (valve 11+14+16) Verifying the probe is dry (removing any residual diluent that may be present by opening (valve 3) Rocker move in the sampling position Syringe up Waste syringe drain (valve 7) Needle down in sampling position



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3103.htm[4/4/2016 10:20:10 AM]



Fluidics



The CELL-DYN Emerald 22 is ready to process another specimen.



  FLUIDICS VIEW All the fluidics part is on the right side of the instrument and consists of only three modules: Sampling Module: Rocker (patent pending) manages the rise and descent of the needle. Syringe Module (patent pending): consists of one block: Reagent syringes (Diluent, lyse), sampling and air syringes. Liquid valve manifold assembly and tubing. Counting chambers WBC and RBC counting chambers and hemoglobin measurement. Liquid valve manifold assembly and tubing.



FLUIDICS PARTS DESCRIPTION The hydraulic part of CELL-DYN Emerald 22 is very simple and made with only 5 modules: Sampling module Counting bath module Syringes module Optical bench *new* Optical manifold *new* Counting bath module WBC and RBC/PLT dilution/counting Prepare the sample for optical measurement. Measure the HGB. Optical manifold This manifold allows driving the different reagents toward the counting bath module or toward the optical bench. It maintains seven commutation valves. Optical bench The illumination module, which contains the semiconductor light source, a beam adjustment glass smear and lens The flow cell



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3103.htm[4/4/2016 10:20:10 AM]



Fluidics



The detection module, which contains lens, a beam separation glass smear, photodiodes and ALL and FSC amplifier boards



  Fluidics Diagram General Diagram



Optical Manifold Tubing and Valves Position



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3103.htm[4/4/2016 10:20:10 AM]



Fluidics



Size and Length Tubing



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3103.htm[4/4/2016 10:20:10 AM]



Fluidics



CELL-DYN Emerald Service and Support Manual (Version 202661-106) • © 2007, 2016 • CELL-DYN, CELL-DYN Emerald and CELL-DYN Emerald 22 are trademarks of Abbott Laboratories in various jurisdictions. • Abbott Park, IL 60064 USA • All rights reserved.



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3103.htm[4/4/2016 10:20:10 AM]



Instrument Alarms, Operational Alerts, and Data Flags



Instrument Alarms, Operational Alerts, and Data Flags Note Refer to the CELL-DYN Emerald Operator's Manual, Principles of the Operation (Section 3).   Note Refer to the CELL-DYN Emerald 22 Operator's Manual, Principles of the Operation (Section 3).  



CELL-DYN Emerald Service and Support Manual (Version 202661-106) • © 2007, 2016 • CELL-DYN, CELL-DYN Emerald and CELL-DYN Emerald 22 are trademarks of Abbott Laboratories in various jurisdictions. • Abbott Park, IL 60064 USA • All rights reserved.



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3104.htm[4/4/2016 10:20:39 AM]



Software



Software Note Refer to the CELL-DYN Emerald System Operations Manual for explanation of the software operation.



Emerald Analyzer USB Key Data Format This section describes the use of a USB key for storing and writing automaton data. Security Specifications To avoid the exchange of private data between different analyzers via USB, such specific files contain the serial numbers of analyzer and CRC. So, verification can be made before upload on data analyzer. The data could only be uploaded onto the right analyzer. Interface Specification The file system type is FAT16. The file and folder names are limited to 8 characters +3 for the file extension. The content files are ASCII and some fields are coded in UTFB: SID, PID, ID, Blood Type, operator, target name. Root Folder The USB key can contain data for multiple automaton. The limit is its capacity. At the root folder level of the USB key, a folder contains all Emerald data type: AB18. Automaton ID Folder Each automaton data group is stored on the USB key inside a unique sub-folder: xxxxxx\yyyyyy = 12 digit Emerald serial number. Inside this sub-folder, the data is private. An automaton can only access its own private and all common data. Private data exchange between two automatons is impossible. USB Key Folder Overview The analyzer serial number is coded as xxxxxx-yyyyyy (MSB-LSB). The root folder (AB18) contains only Emerald type analyzer data only, USB Key Structure shows the file structure of the USB key: USB Key Structure



USB Key Data Files Overview



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3105.htm[4/4/2016 10:21:12 AM]



Software



The USB key contains both PRIVATE and COMMON data. PRIVATE Data Inside AB18\XXXXXX\YYYYYY Folder



Folder



File Name



Contents



Comments



Format, unit



Analyzer CRC Read/Write Control



CALI



221OGb.CAL (calibration date DDMMYY)



Calibration report = calibration factors + target + results



One file per calibration date



Ascii + UTF-8 current unit



YES/NO



NO



LOGS



LOG_ERRCSV LOG.CSV



Error + Service Logs



Two files



Ascii



YES/NO



NO



QC



CRXV6875QCN (target name. level)



QC report = target + One file per QC target and level results



Ascii + UTF-8 STD unit



YES/YES



YES



EQC



ID.DAT LOWQCDAT NORMQCDAT HIQCDAT



ID file + EQC results



One sub folder per target. One file per level + ID file



Ascii ABBOTT format STD (USA)



YES/NO



NO



RESULTS



0047CSV (sequence number CSV)



Result analysis



One sub folder per Year/month (YYYY-MM), One sub folder per day (DD), One file per result Limited to 510 files



Ascii + UTF·8 current unit



YES/YES



YES



SETTINGS



TECH.SET



Private analyzer settinqs



One file



Ascii + UTF·8



YES/YES



YES



Private reagents log



One file



Ascii



YES/YES



YES



REAGENTS REAGLOGS.CSV COMMON Data Inside ABl8 Folder



Folder



File Name



Contents



Comments



Format, unit



Analyzer Read/Write



CRC Control



LOTS



MXC1234.QCN MXBC02174.QCL (target name.level) CAL0233.CAL (target name.CAL)



QC target values and Calibration target values



One file per target, limited to 100 files



Ascii ABBOTT format STD (USA)



NO/YES



NO



SETTINGS



USER.SET



Common settinqs



One file



Ascii + UTF·8



YES/YES



YES



UPDATE\VERSION V100-004.b (version release software name.b)



Software release



One file per software release



binary



NO/YES



YES



UPDATE\PRVDRV



Printer drivers



One file per printer driver



binary



NO/YES



YES



HP6122.LPT (prn_name.port LPT or USB)



Specific Technician Display This section describes the software operations which are only accessible with the service password. User Access Menus



Links Emerald Software Tree 3H_8100.PDF Service Password The service password is: AFSE. Note It is strictly forbidden to disclose the password to the customer. The service displays are accessible from MAIN SCREEN then MAINTENANCE then ABBOTT SERVICE.



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3105.htm[4/4/2016 10:21:12 AM]



Software



Service Screen The following activities are available in the service screen: COUNTING PARAMETERS - adjustment of the counting parameters and lyse quantity. These adjustments are possible with the Factory Access Level only. APERTURE CURRENT OFF - test the aperture current. SET GAINS - latex adjustment and HGB blank adjustment. PARK SYRINGE - to park pistons prior to transport. SET SERIAL NUMBER - enter analyzer serial number. LOG. INTERVENTION - fill up the log accessible from the MAIN MENU. Set Gains Screen The following activities are available in the set gains screen: BURN-IN - Not available: manufacturing parameters. ADJUST HGB - adjust the HGB blank level. WBC GAIN - adjust the WBC gain with the use of special latex particles. RBC PLT GAIN - adjust the RBC and PLT gain with the use of special latex particles. AUTO (check box) - when checked, adjustments are automatically performed.



CELL-DYN Emerald 22 Software



Software Exchange Interface Specifications



Folders Overview on the USB Key The analyzer serial number is coded as xxxxxx-yyyyyy (MSB-LSB).



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3105.htm[4/4/2016 10:21:12 AM]



Software



Data Files Overview on the USB Key



PRIVATE DATA in AB22\xxxxxx\yyyyyy Folder



Calibration report = calibration factors + target + results



One file per calibration date



Ascii + UTF-8 current unit



 



CRC Control  



NO/YES



NO



LOG_ERR.CSV LOG.CSV CRXV6875.QCN (target name.level)



Error + service logs QC report = target + results



Two files



Ascii



NO/YES



NO



One file per QC target and level



Ascii + UTF-8 STD unit



YES/YES



YES



ID.DAT LOWQC.DAT NORMQC.DAT HIQC.DAT



ID file + EQC results



Ascii ABBOTT format STD (USA) unit



 



 



NO/YES



NO



0047.CSV (sequence number.CSV)



Result analysis



 



 



YES/YES



YES



TECH.SET



analyser settings



One sub folder per target = lot name , 1 file per level + ID file One sub folder per Year/month (YYYY- MM), One sub folder per day (DD), One file per result Limited to 510 files One file



YES/YES



YES



USER.SET



User settings



One file



YES/YES



YES



REAGLOGS.CSV 0047.CSV (sequence number.CSV)



Private reagents log Result analysis with Raw information.



One file One sub folder per Year/month (YYYY- MM), One sub folder per day (DD), One file per result Limited to 510 files



YES/YES  



YES  



NO/YES



YES



Files Name (example) 221006.CAL (calibration date DDMMYY)



Folder CALI



LOGS QC



EQC



RESULTS



SETTINGS



REAGENTS RAW



Contents



Stored under specific conditions.



Comments



Format, Unit



Ascii + UTF-8 current unit Ascii + UTF-8 Ascii + UTF-8 Ascii Ascii + UTF-8 current unit



Analyzer Read/Write



COMMON DATA in AB22 Folder



Folder



Files



Contents



Comments



CRC Format, Analyzer Read/Write Control Unit Ascii   ABBOTT YES/NO format NO USA (STD)unit



Qc target values & Calibration target values



One file per target Limited at 100 files



UPDATE\ V100-004.b VERSION ([version name].b)



Software release



One file per software release



binary



UPDATE\ HP6122.LPT PRTDRV (prn_name.port LPT or USB )



Printer drivers



One file per printer driver



binary



LOTS



MXC1234.QCN MBC02174.QCL (target name.level) CAL0233.CAL (target name.CAL)



 



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3105.htm[4/4/2016 10:21:12 AM]



YES/NO YES/NO



YES YES



Software



CELL-DYN Emerald Service and Support Manual (Version 202661-106) • © 2007, 2016 • CELL-DYN, CELL-DYN Emerald and CELL-DYN Emerald 22 are trademarks of Abbott Laboratories in various jurisdictions. • Abbott Park, IL 60064 USA • All rights reserved.



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3105.htm[4/4/2016 10:21:12 AM]



Troubleshooting (Document Control Number 202664-103)



CELL-DYN Emerald Service and Support Manual (Version 202661-106) • © 2007, 2016 • CELL-DYN, CELL-DYN Emerald and CELL-DYN Emerald 22 are trademarks of Abbott Laboratories in various jurisdictions. • Abbott Park, IL 60064 USA • All rights reserved.



https://gssweb-d.abbott.com/WWCS/hematology/emerald_html/32.htm[4/4/2016 10:53:32 AM]



Drawings and Schematics



Drawing/Schematic Name CELL-DYN Emerald 18



Link



Electronic Diagram



3H_9001.PDF



Fluidics Block Diagram



3H_9005.PDF



Wiring Diagram - General View



3H_9003.PDF



Wiring Diagram - Physical View



3H_9004.PDF



Drawing/Schematic Name CELL-DYN Emerald 22



Link



Electronic Diagram



3H_1001.PDF



Parts List



3H_1002.PDF



Fluidics Block Diagram Wiring Diagram - General View Software Structure



 



CELL-DYN Emerald Service and Support Manual (Version 202661-106) • © 2007, 2016 • CELL-DYN, CELL-DYN Emerald and CELL-DYN Emerald 22 are trademarks of Abbott Laboratories in various jurisdictions. • Abbott Park, IL 60064 USA • All rights reserved.



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3202.htm[4/4/2016 10:22:01 AM]



CELL-DYN EMERALD 22 TROUBLESHOOTING



CELL-DYN Emerald 22 TROUBLESHOOTING OPTICS TROUBLESHOOTING Note if anything dries inside the nozzle, it will be nearly impossible to clear out.  The analyzer must be shut down or prepared for storage properly.  Remember, this system is heated, which can dry any residual diluent in the flow cell in a relatively short time.



  HYDRAULICS PROBLEM 1. Check all functions of the solenoid valves (Service > Mechanics > Check Valves>Open/close).  You should distinctly hear the state changing. 2. Check the different steps in the WBC bath (draining, filling, dilution, bubble mix, lysing, etc.). 3. Verify tubing #48 is positioned properly, with a loop against the optics assembly. This positioning will ensure micro bubbles do not flow back into the injector during the measurement. They will remain in the high part of the loop and be evacuated at the end of the cycle. 4. Verify tubing #48 is emptied and filled properly during the cycle. a. At the beginning of the cycle, at the end of the WBC bath emptying, tubing #48 should be empty. b. During the cycle, just after the second mixing (after lyse addition) in the WBC bath, tubing #48 must be completely filled with the blood sample present in the WBC bath. Inspect the tee fitting on the WBC bath for damage. This could cause a restriction and not enough events. Note Material is being changed from polypropylene to nylon. Check tubing #56, 55 and 47 for leaks. Inspect tubing # 37, 5, 6, because they can be pinched when the fluidic door is closed, causing problems for tubing #48 emptying and filling. 5. Blockage in tubing #50 Tubing 50 can cause challenging troubleshooting problems.  It should only contain lyse, but if there is a a leak, the sample, rather than staging properly, may get pulled into tubing 50 and contaminate the lyse bottle.  If there is an obstruction, the staged sample may get pulled straight to waste.



  FLOWCELL PROBLEM 1. Check for an air bubble at the top of the nozzle. 2. Remove the flow cell protection. 3. Run a cycle and observe the top of the nozzle during the measurement.  If an air bubble is at the top of the nozzle, it will diffract the light and you will observe a bright blue spot at the nozzle exit. 4. Run an empty cycle and pinch simultaneously the two diluent tubings (# 52 and 53) just under the flow cell support during the measurement.  This will create a pressure difference which will unblock the air bubble. 5. Try putting dil tubing in cleaner and do a prime.  Do in class w/ dil first then cleaner-see if bubs go away. 6. Repeat empty to see if the air bubble comes back. 7. Verify that the nozzle is not blocked. 8. Repeat empty to see if the air bubble comes back. 9. Remove the flow cell from the module. Note https://gssweb.abbott.com/WWCS/hematology/emerald_html/3203.htm[4/4/2016 10:29:20 AM]



Principle of Optical Bench Alignment



Principle of Optical Bench Alignment



Optical Bench Origin of the Alignment Particles to be analyzed are transported by the 80 µm cross section sample flow and pass the optical interrogation zone, where light-particle interaction occurs:



To get the best possible SNR (Signal Noise Ratio) for the optical measurement, dimensions of the optical interrogation zone have to be close to the sample flow diameter. The optical interrogation zone is a rectangular area of 110 µm x 30 µm, the sample flow has a diameter of 80 µm, and their relative positioning has a tolerance of ± 15 µm. Such a tolerance cannot be reached directly when stacking the several mechanical parts of the optical bench: An alignment is required. The alignment basically comes from the need for high accuracy positioning of the light beam with respect to the sample flow. Principle of the Alignment The flow cell is static (i.e., the sample flow position is fixed), but the light beam is shifted either longitudinally or transversally for the optical interrogation zone to match the sample flow:



Longitudinal Alignment This is a factory procedure requiring specific equipment. The longitudinal alignment is done when the system is assembled and never requires further adjustment. Maintenance or replacement of the flow cell has no impact on longitudinal alignment. If the screws that lock the illumination module alignment are loosened or removed, the optical bench must be replaced and the original optics sent to the factory for full realignment. Never loosen the illumination module screws. Transverse Alignment The transverse position of the light beam with respect to the fixed sample flow (see the previous image) must be adjusted whenever the flow cell is removed or replaced.



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3107.htm[4/4/2016 10:18:48 AM]



Principle of Optical Bench Alignment



The light beam transverse position is adjusted by rotating the transverse adjustment window. Any rotation of the window holder (represented in green in figure 3H_7002 below) transversally shifts the light beam (represented in green in figure 3H_7001).



The sample flow nominal position is represented in figure 3H_7003, and the sample flow transverse position is correct when its cross section is fully included inside the interrogation zone as represented in figure 3H_7004. Thus, the transverse adjustment is a process that moves the light beam from a random transverse position to the nominal one represented in figure 3H_7004. Transverse adjustment relies on the use of Latex beads with a mean diameter of 7 µm. The latex beads have diameter variations of less than 1% from one bead to the next and can be considered identical.



Since the particles are identical in size and shape, the light pulses generated by the optics should also be identical when the system is properly aligned (as represented in figure 3H_7006f). If the sample flow is not fully included in the interrogation zone (figure 3H_7003), some of the latex beads will not be fully illuminated because they are located at the zone boundary or edge of the detection area (see particle #3 in figure 3H_7003).



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3107.htm[4/4/2016 10:18:48 AM]



Principle of Optical Bench Alignment



When the system is not properly aligned, particles receive different amount of light, resulting in pulses of various amplitude (figure 3H_7005). When properly aligned, sample flow is fully included inside the interrogation zone (figure 3H_7004), and all particles receive the same amount of light. Pulses amplitude are then very similar, with only a very slight difference between the highest and lowest (figure 3H_7006). This slight remaining difference comes from the baseline residual noise (figure 3H_7008). Embedded Alignment Tool The instrument embedded tool for transverse alignment works on batches of 10 latex pulses. For every batch of 10 pulses, the MAX and MIN values are found and plotted on the graph (see figures 3H_7007 and 3H_7008). The MAX value is plotted in red, whereas the MIN value is plotted in black.



The red graph represents the MAX value of each packet of 10 particles while the black graph represents the MIN values of those batches. The peaks on the red graph in figures 3H_7007 and 3H_7008 occur when more than one latex bead enters the interrogation zone. These peaks (on the red only) have no effect on the alignment accuracy. When the light beam is not aligned properly, the pulse amplitude has high dispersion (figures 3H_7005 and 3H_7007), and the black MIN graph value varies over time because it represents the particle outside the zone. When the system is properly aligned (figures 3H_7006 and 3H_7008), all particles are equally illuminated and dispersion of the black MIN graph is very low (figure 3H_7009).



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3107.htm[4/4/2016 10:18:48 AM]



Principle of Optical Bench Alignment



To align the light beam transversally to the sample flow, simply rotate the holder (figure 3H_7002) while both curves are updating. The transverse alignment is complete when fluctuations on the black graph (MIN) appear similar to those depicted in figure 3H_7008. Note The diameter and length of the nozzle will result in a slightly different appearance of optics from instrument to instrument. Spec is 80 =/- 10 µm. Due to the spec tolerance, it isn’t possible to come up with an exact center for the neutral population. Both examples below are correct and may be how the same specimen is represented on two different analyzers.



Note This is whole blood, not Gcal. Additional optimization is possible with future software revisions. The goal is to be able to optimize with whole blood. Seeming subtle adjustments can make a big difference in flagging and QC difference results.



The FSC is too high (approximately +8), which can result in lymphs being classified as basos.



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3107.htm[4/4/2016 10:18:48 AM]



Principle of Optical Bench Alignment



A-OK.



The FSC is too low, which can result in lymphs falling in the N2 range or in excessive N2 flagging.



CELL-DYN Emerald Service and Support Manual (Version 202661-106) • © 2007, 2016 • CELL-DYN, CELL-DYN Emerald and CELL-DYN Emerald 22 are trademarks of Abbott Laboratories in various jurisdictions. • Abbott Park, IL 60064 USA • All rights reserved.



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3107.htm[4/4/2016 10:18:48 AM]



CELL-DYN EMERALD 22 TROUBLESHOOTING



DO NOT PUT FINGERS ON OPTICAL GLASS. 10. Connect syringe full of distilled water at the entrance of tubing #48 at the base of the flowcell.



11. Block the entrance of tubing #47 and push on the syringe. The stream should be straight as it exits the nozzle. If the flow is bent, it means that the nozzle is partially blocked. If there is no flow, it means that the nozzle is totally blocked.



12. Verify flow cell lenses are not dirty, scratched or damaged. Note A scratch on the lens can make the transverse support adjustment very difficult. The min/max in the adjustment menu (OPTICAL ADJUST) may be very noisy, and the automatic gain adjustment may not work.



13. Remove the flowcell and inspect the lenses (with a microscope, if necessary). 14. Clean with an alcohol solution or optic tissue. Never clean inside or out with “Spectrum cleaner.” Example: N2 flags on every specimen. Problem: Partially blocked flowcell:



ELECTRONICS PROBLEM The FSE first believes that the problem is with the flow cell or optics…but it can be a problem with the FSC detection. FSC is the trigger for ALL. If the FSC cable is damaged or has a bad connection, or something is wrong with the CPU control of the FSC, then there will be no ALL signal observed. The signal will be there, but it can’t be seen without an oscilloscope.   https://gssweb.abbott.com/WWCS/hematology/emerald_html/3203.htm[4/4/2016 10:29:20 AM]



CELL-DYN EMERALD 22 TROUBLESHOOTING



ANALYTICAL PROBLEMS PARAMETERS WBC



PROBLEMS No results



CONDITIONS No HGB



 



 



HGB OK



 



Bad stability



 



5 DIFF



No result



No HGB & WBC



 



 



HGB & WBC OK



RBC



No results



No HCT & PLT



 



Bad stability



HCT & PLT too



HGB  



No results Bad stability



   



SOLUTIONS Check the lysis level. Check the right lysis tubing connection to the WBC bath. Check the valve Nb 9. Check the bath wires. Perform a Cleaning Cycle and then a Bleach cycle, if unsuccessful. Change the aperture block, if unsuccessful. Perform a Back flush and a Cleaning Cycle and then a Bleach cycle, if unsuccessful. Check the level bubble flow in the WBC bath during the run cycle. Check the reagent and enclosure temperature. Check the lysis level. Check the right lysis tubing connection to the WBC bath. Check the valve Nb 9. Perform a Back flush Cycle and then a Bleach cycle, if unsuccessful. Check the reagent and enclosure temperature. Check the preamplifier connection wires. Check the bath wires. Perform a Cleaning Cycle and then a Bleach cycle, if unsuccessful. Perform a Back flush and a Cleaning Cycle and then a Bleach cycle, if unsuccessful. Check the level bubble flow in the RBC bath  during the run cycle. Check the level bubble flow in the WBC bath  during the first dilution. Check the led light on. Check if no bubble in the lysis tubing. Check the reagent and enclosure temperature. Check the level bubble flow in the WBC bath during the run cycle.



OTHER PROBLEMS ORIGIN EMERALD              



PROBLEMS Diluent leaks around the needle during the run cycle No starting All results bad No display Machine switch off after few second following the switch on. No display ID and/or PID typing impossible Message : TEMPERATURE OUT OF



SOLUTIONS Check the rinsing needle block (for the presence of clots) and clean it, if necessary. (See section 9.3.2.) Check the power supply connection wires. Check the level diluent and if the tubing is pinched. Check if the diluent is at the same level than the analyzer. Check the flat cable. See section 4.5.2. Check the flat cable. ID and/or PID are mandatory. (See section 3.4.3.) Check the origin of the problem of temperature in section 9.3.



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3203.htm[4/4/2016 10:29:20 AM]



CELL-DYN EMERALD 22 TROUBLESHOOTING



RANGE or flag INS_T>



 



Message: CYCLE: PRESSURE DEFAULT or Flag INS_P



Trigger temperature values:             Ambient: 17°C 35°C             Enclosure: 32°C 40°C             Reagents: 34°C 45°C



Cases of occurrence: Drain chamber default: Check the tubing connection of the fluidics.  



 



Rinsing head default: Check the in line filter locking (see section 9.1.4) and the pump operation.



 



Counting vacuum default: Perform a piston greasing (see section 9.1.3) and check the tubing connection of the fluidics. Note For all occurrences also check the level of the reagents.



Printer



No printing



 



Bad printing



Check the paper. Check the electrical connection. Check the black ribbon.



TROUBLESHOOTING MESSAGES    MESSAGE *** SYSTEM: FATAL ERROR https://gssweb.abbott.com/WWCS/hematology/emerald_html/3203.htm[4/4/2016 10:29:20 AM]



ACTION N/A Restart the Analyzer.



CELL-DYN EMERALD 22 TROUBLESHOOTING



SYSTEM: INTERNAL TIME OUT



Restart the Analyzer.



CYCLE: BUSY CYCLE STOPPED BY USER CYCLE: EMERGENCY STOP CYCLE: CMD VALVE FAILED CYCLE: VALVE 1 FAILED CYCLE: VALVE 2 FAILED CYCLE: VALVE 3 FAILED CYCLE: VALVE 4 FAILED CYCLE: VALVE 5 FAILED CYCLE: VALVE 6 FAILED CYCLE: VALVE 7 FAILED CYCLE: VALVE 8 FAILED CYCLE: VALVE 9 FAILED CYCLE: VALVE 10 FAILED CYCLE: VALVE 11 FAILED CYCLE: VALVE 12 FAILED CYCLE: VALVE 13 FAILED CYCLE: VALVE 14 FAILED CYCLE: VALVE 15 FAILED CYCLE: VALVE 16 FAILED CYCLE: VALVE 17 FAILED CYCLE: VALVE 18 FAILED CYCLE: INIT NOT DONE OUT OF RANGE MECA: MOTOR NEEDLE BUSY MECA: MOTOR SYRINGE BUSY MECA: MOTOR ROCKER BUSY MECA: HOME NEEDLE NOT FOUND MECA: HOME SYRINGE NOT FOUND MECA: HOME ROCKER NOT FOUND MECA: MOTOR NEEDLE GAP MECA: MOTOR SYRINGE GAP MECA: MOTOR ROCKER GAP MECA: NEEDLE NOT IN TOP POSITION CONTROL CYCLE NOT DONE START UP CYCLE NOT DONE RINSE NOT DONE CLEAN NOT DONE STARTUP CYCLE FAILED CYCLE: FLUIDIC DOOR OPENED INTERN: COUNT ERROR RUNNING AUTO CLEANING DILUENT ALMOST EMPTY LYSE ALMOST EMPTY



Wait before performing a cycle. Emergency stop, please perform a control cycle. Perform a control cycle. Change the valve. Change the valve. Change the valve. Change the valve. Change the valve. Change the valve. Change the valve. Change the valve. Change the valve. Change the valve. Change the valve. Change the valve. Change the valve. Change the valve. Change the valve. Change the valve. Change the valve. Change the valve. Change the valve. Perform an initialization or a control cycle. Modify the value. Restart the Analyzer. Restart the Analyzer. ReStart the Analyzer. Perform an initialization or a control cycle. Perform an initialization or a control cycle. Perform an initialization or a control cycle. Nothing. Perform  a pistons greasing. Nothing. Perform a control cycle. Perform a control cycle. Perform a start up cycle. Perform a clean cycle. Perform a rinse cycle. Perform a new start up cycle. Close the Fluidic door. Run Control cycle. Restart the Analyzer. Press OK. Replace the container and perform a prime Diluent. Replace the bottle and perform a prime Lysis.



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3203.htm[4/4/2016 10:29:20 AM]



CELL-DYN EMERALD 22 TROUBLESHOOTING



CLEANER ALMOST EMPTY WASTE ALMOST FULL THE DILUENT USED IS OUT OF DATE. THE LYSE USED IS OUT OF DATE. THE CLEANER USED IS OUT OF DATE. SYSTEM: EEPROM COM ERROR INIT PRINTER NO PRINTER SELECTED PRINTER IS BUSY PRINTER: NO PAPER NO PRINTER SELECTED PRINTER IS OFF NO PRINTER RESPONSE PRINTER ERROR INTERN: RESULT AREA IS LOCKED. BACKUP: SYSTEM ERROR HARDWARE: SYSTEM ERROR ANALYSIS MODE UNKNOWN BACKUP: NO MEMORY AVAILABLE FOR STORAGE BACKUP: MEMORY IS ALMOST FULL. PLEASE DELETE RESULTS. BACKUP: BAD FOLDER DUPLICATION BACKUP: CALIBRATION HISTORY IS FULL BACKUP: REPEATABILITY HISTORY IS FULL BACKUP: QC HISTORY IS FULL INTERN: NO MEMORY AVAILABLE INTERN: MEMORY CORRUPTED COM: CRC CONTROL ERROR RS232: TIME OUT RS232: INTERNAL ERROR RS232: SYNCHRO ERROR RS232: ACK ERROR SVM: COM. TIME OUT SVM: UNMATCH SVM: WG SVM: XB SVM: BAD VERSION SVM: ILLEGAL SERIAL NUMBER. ID AND/OR PID MANDATORY (CHECK SETUP). SID ALWAYS MANDATORY. PRESSURE DEFAULT PRESSURE DEFAULT : NEEDLE RINSING DEFAULT PRESSURE DEFAULT : DRAIN BATH DEFAULT PRESSURE DEFAULT : VACUUM COUNTING DEFAULT PRESSURE DEFAULT : NO REAGENT PRESSURE DEFAULT : SYRINGE VACUUM https://gssweb.abbott.com/WWCS/hematology/emerald_html/3203.htm[4/4/2016 10:29:20 AM]



Replace the bottle and perform a prime Cleaner. Replace the waste container. Replace the container and perform a prime Diluent. Replace the bottle and perform a prime Lysis. Replace the bottle and perform a prime Cleaner. Restart the Analyzer. Switch on the printer or invalidate the printings. Switch on the printer or invalidate the printings. Switch on the printer or invalidate the printings. Add some paper. Switch on the printer or invalidate the printings. Switch on the printer or invalidate the printings. Switch on the printer or invalidate the printings. Switch on the printer or invalidate the printings. Wait before performing a cycle. If persisting, restart the Analyzer. Restart the Analyzer. Restart the Analyzer. N/A. Analysis Mode selected is unknown. This could happen on SVM mode. Delete the stored results. Delete results. Restart the Analyzer. Delete the calibration results. Delete the repeatability results. Delete the Q.C. results of the ongoing lot. Restart the Analyzer. Restart the Analyzer. Communication error retry. Restart the Analyzer. Restart the Analyzer. Restart the Analyzer. Restart the Analyzer. Restart the SVM. Reenter the file or confirm it (manual connection on the SVM). Westgard alarm. XB alarm. Update the SVM software. This Analyzer cannot be connected to the SVM. Enter an ID and/or PID and SID. May have occured by leak of reagent, or check tubing in the fluidics. Check the filter locking and the pump operation. Check the tubing connection of the fluidics. Perform a piston greasing and check the tubing connection of the fluidics. Check the reagents level. Change the valve.



CELL-DYN EMERALD 22 TROUBLESHOOTING



PRESSURE DEFAULT : WASTE DRAINING CYCLE: VALVE 7 FAILED CYCLE: VALVE 8 FAILED CYCLE: VALVE 9 FAILED CYCLE: VALVE 10 FAILED CYCLE: VALVE 11 FAILED BACKUP : FOLDER NOT FOUND BACKUP: LAST SETUP RESTORED. BACKUP: SETUP UPDATED WITH DEFAULT VALUES. BACKUP: PARTIAL NUM DELETED. BACKUP: PARTIAL CALI. RES. DELETED. BACKUP: PARTIAL REPET. RES. DELETED. BACKUP: PARTIAL QC RES. DELETED. BACKUP: LAST RESULTS RESTORED. BACKUP: LAST QC RESTORED. BACKUP: FILE SYSTEM FAILED. BACKUP: SECTOR FAILED. COM: BAD CYCLE MODULE HARDWARE: F.S.C BOARD ID FAILED. HARDWARE: A.L.L BOARD ID FAILED. HARDWARE: HEAT REAGENT STOPPED. HARDWARE: HEAT ENCLOSURE STOPPED. HARDWARE: HEAT REAGENT FAILED. HARDWARE: HEAT ENCLOSURE FAILED. HARDWARE: ENCLOSURE FAN FAILED. SYSTEM LOCKED HEATING FAILED TEMPERATURE OUT OF RANGE HEATING IN PROGRESS, PLEASE WAIT. SET TEMPERATURE REACHED. CYCLE: HGB CHANNEL SATURATION. PLEASE RUN STARTUP. COM: SIZE ERROR. SYSTEM: DOWNLOADING NEW VERSION. PLEASE WAIT USB: UNABLE TO OPEN FILE USB: THUMB DRIVE IS FULL. USB: EMPTY FILE USB: THUMB DRIVE IS NOT PRESENT. USB: WRITE PROTECTED FILE. USB: TOO MANY FILES OPENED. USB: DIRECTORY DOES NOT EXIST. USB: UNABLE TO OPEN DIRECTORY. USB: DIRECTORY IS NOT EMPTY. USB: THUMB DRIVE I/O ERROR. USB: UNABLE TO CREATE DIRECTORY. SETUP: MODIFICATION NOT ALLOWED. https://gssweb.abbott.com/WWCS/hematology/emerald_html/3203.htm[4/4/2016 10:29:20 AM]



Change the valve. Change the valve. Change the valve. Change the valve. Change the valve. Change the valve. Restart the Analyzer. Indicate that your SETUP has been restored. Indicate that your SETUP has been released. A bad Result storage happened. The last analysis has not been stored. A bad Result storage happened. The last CALI analysis has not been stored. A bad Result storage happened.  The last REPET. analysis has not been stored. A bad Result storage happened. The last QC analysis has not been stored. File has been restored to previous state. File has been restored to previous state. Restart the Analyzer. Hardware failure on memory. Rebuild cyles with good options. Check the hardware connection on FSC Board. Check the hardware connection on ALL Board. Check your reagent sensor. Check your enclosure sensor. Call an Abbott representative. Call an Abbott representative. Check if your temperature fan is running. Call an Abbott representative. Room température out of range (34°C). Wait for the system to reach its temperature. The instrument is ready to work. Run Startup Cycle. If the problem still occurs, Call an Abbott representative. Try again, if the problem ocur, call an Abbott representative. System is resetting after the version released. The USB thumbdrive as an incompatible tree structure for the analyzer, try another. No memary available in USB for storage. You have to delete some data to work. The USB thumbdrive as an incompatible tree structure for the analyzer, try another. Connect a USB thumbdrive. The USB thumbdrive as an incompatible tree structure for the analyzer, try another. The USB thumbdrive as an incompatible tree structure for the analyzer, try another. The USB thumbdrive as an incompatible tree structure for the analyzer, try another. The USB thumbdrive as an incompatible tree structure for the analyzer, try another. The USB thumbdrive as an incompatible tree structure for the analyzer, try another. The USB thumbdrive as an incompatible tree structure for the analyzer, try another. The USB thumbdrive as an incompatible tree structure for the analyzer, try another. You have to be Logged with the good access code.



CELL-DYN EMERALD 22 TROUBLESHOOTING



BACKUP: LAST RESULT SAVED. BACKUP: REPAIR SECTOR DONE. BACKUP: FAIL TO SAVE RESULT ONTO THE EXTERNAL STORAGE DEVICE. THE INTERNAL MEMORY IS USED. INVALID DATA FORMAT. LOT ALREADY EXISTS. ACTION CANCELLED. NUMBER MAX. OF FILES REACHED. PLEASE DELETE FILES. VERSION RELEASE FAILED. THE CHOSEN RELEASE IS NOT COMPATIBLE WITH THE EMERALD 22 SYSTEM. PRINTER DRIVER UPDATE FAILED. THE CHOSEN DRIVER IS NOT COMPATIBLE WITH THE EMERALD 22 SYSTEM. NETWARE:  SERVER INIT. FAILED NETWARE: CLIENT INIT. FAILED. HOLDER: CLOSE DOOR TO ADJUST. HOLDER:  TOO MUCH STEP DONE. ADJUST CYCLE FAILED. HOLDER: ERROR WHILE OPENING. HOLDER: BAD POSITION. AUTO ADJUSTMENT FAILED. HOLDER: CLOSING ERROR HOLDER: NO TUBE HOLDER DETECTED. HOLDER: OPENING ERROR CYCLE: VALVE 19 FAILED CYCLE : PUMP COMMAND  FAILED. CYCLE: VALVE 12 FAILED CYCLE: VALVE 13 FAILED CYCLE: VALVE 14 FAILED CYCLE: VALVE 15 FAILED CYCLE: VALVE 16 FAILED CYCLE: VALVE 17 FAILED CYCLE: VALVE 18 FAILED CYCLE: VALVE 19 FAILED HARDWARE: BACK FAN FAILED. CHECK [SET UP/ADVANCED/INSTRUMENT ID] AND/OR PIERCING CONNECTION. CYCLE: HGB CHANNEL SATURATION. A USB THUMB DRIVE IS ALREADY MOUNTED ON PORT 2. PLEASE REMOVE THE THUMB DRIVE FORM THE PORT 1. A USB THUMB DRIVE IS ALREADY MOUNTED ON PORT 1. PLEASE REMOVE THE THUMB DRIVE FORM THE PORT 2. THE USB THUMB DRIVE FILE SYSTEM IS NOT COMPATIBLE WITH THE EMERALD 22. EITHER REMOVE IT OR FORMAT IT. THE USB DEVICE PLUGGED IN PORT 1 IS NOT COMPATIBLE WITH THE EMERALD 22. PLEASE REMOVE IT FROM PORT 1. THE USB DEVICE PLUGGED IN PORT 2 IS NOT COMPATIBLE WITH THE EMERALD 22. PLEASE REMOVE IT FROM PORT 2. A USB PRINTER IS ALREADY PLUGGED IN PORT 2.PLEASE REMOVE THE https://gssweb.abbott.com/WWCS/hematology/emerald_html/3203.htm[4/4/2016 10:29:20 AM]



Memory full, next result will not be saved. You have to delete results. Internal database as been restored after detecting incoherent. Check if the USB thumbdrive is connected to the analyser. Retrieved data is invalid. The readen dat is not compatible with the analyzer. Select another lot. Delete data on the selected directory. Select the correct version. Select the right driver compatible with the Analyzer. Check your analyser configuration. Check your analyser configuration. Message reserved to the service people. Message reserved to the service people. Check if something prevent the door opening. Message reserved to the service people. Check if something prevent the door closing. Put the tube holder. Check if something prevent the door opening. Change the valve. Check the pump connexion. Change the valve. Change the valve. Change the valve. Change the valve. Change the valve. Change the valve. Change the valve. Change the valve. Check if your back fan is running. Check piercing connection or set model or serial number. Check the HGB adjustment. Remove one USB key. Remove one USB key. Change the valve. Check that your USB key is sompatible with the analyzer. Check that your USB key is sompatible with the analyzer. Unplug the USB item.



CELL-DYN EMERALD 22 TROUBLESHOOTING



PRINTER FROM THE PORT 1. A USB PRINTER IS ALREADY PLUGGED IN PORT 1. PLEASE REMOVE THE PRINTER FROM THE PORT 2. TUBE HOLDER SWITCH ERROR< THE DILUENT REAGENT LOG IS FULL THE LYSE REAGENT LOG IS FULL THE CLEANER REAGENT LOG IS FULL FAILED TO LOG THE REAGENTS LOG FILE. THE FILE IS NOT PRESENT OR INVALID IHM : ERROR INIT EPSON DILUENT REAGENT IS EMPTY. PLEASE REGISTER A NEW CONTAINER OF DILUENT REAGENT. LYSE REAGENT IS EMPTY. PLEASE REGISTER A NEW CONTAINER OF LYSE REAGENT. CLEANER REAGENT IS EMPTY. PLEASE REGISTER A NEW CONTAINER OF CLEANER REAGENT. THE WASTE IS FULL. PLEASY EMPTY WASTE CONTAINER AND RESET.



Unplug the USB item. Call an Abbott representative. Diluent reagent log is full. It must be deleted. Lyse reagent log is full. It must be deleted. Cleaner reagent log is full. It must be deleted. The reagent log is full. No more reagent entry could be added. Tech Error: error during Screen  init Replace the diluent reagent. Replace the lyse reagent. Replace the cleaner reagent. The waste has to be flushed.



 



CELL-DYN Emerald Service and Support Manual (Version 202661-106) • © 2007, 2016 • CELL-DYN, CELL-DYN Emerald and CELL-DYN Emerald 22 are trademarks of Abbott Laboratories in various jurisdictions. • Abbott Park, IL 60064 USA • All rights reserved.



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3203.htm[4/4/2016 10:29:20 AM]



SPECI AL MODES



PRECI SI ON



LI NEARI TY



3H_8100a



Preamplifier Board



Aperature Voltage WBC IN



RBC IN



+5V +12V -12V



WBC Amplifier



+5V +12V -12V +60V WBC Out



RBC Amplifier PLT Amplifier



Electronic Block Diagram



CPU Board



Gain Control



HGB Amplifier



RBC Out



User Interface Board



Peak Detectors



Display & Keyboard Controller



CPU



ADC



PLT Out



HGB IN HGB Out HGB LED Supply +5V



Interface & Level Translation Logic



Diluent TEMP OUT Diluent TEMP IN



Syringe Block



WBC Threshold



Threshold Control



RBC Threshold



Threshold Comparators



WBC Pulse



PLT Low Threshold PLT High Threshold



RBC Pulse



+ -



PLT Pulse



Pulse Processing Logic



Processing & Storage Memory



Communications Perhipherals



Motor & Electrovalve Drivers



Needle Block



Reference Voltage



+60V Aperature Voltage +5V Power Adapter AC / DC



+24VDC



DC / DC Converters



+3.3V +12V



Rocker Block



-12V Counting Block Parallel Port



Serial Port



Bar Code Reader



Ethernet



USB



USB 3H_9001a



Wiring Diagram - General View 1 Fluidic Door Switch



2



Probe Optical Sensor



3 Stepper Motor Cable



S EV



Syringe



4



C EV Syringe Valves Cable



Rocker



Needle



5



SW



Syringe Optical Sensor



6



3



6



Stepper Motor Cable Rocker Optical Sensor



50P Flex Cable



3



Stepper Motor Cable



J2 Counting Valves Cable



7



11 J3



HB



C



RB



Temperature Sensor



BC



8



W



Start Key Switch



10 Counting Chambers



9



3H_9003b



Wi r i ngDi agr am -Phy s i c al Vi ew DoorSwi t ch Syr i ngeEV



Count i ngEV



Needl eOpt i calSensor



RockerOpt i calSensor



Needl eMot or



Mot orSyr i nge



RockerMot or



Syr i ngeOpt i calSensor



3H_9004a



1



2



3



4



5



6



FLUIDICS DIAGRAM



7



8



PRESSURE SENSOR



A



A



9 22 8



21



3 T° Sensor



4



2



1



10



11 EV8



17



19



20



2



12



14 5



17



13



6



C



21



EV6



B



6



11



16



C



5



8 WASTE SYRINGE



5



7



9



WASTE SYRINGE



8



WASTE EV7



EV4



SAMPLING SYRINGE



20



3



EV3



EV9



12



13



LYSE SYRINGE



EV5



B



4



DILUENT



DILUENT SYRINGE



10



19 EV1



EV2



18



18



EV10



14



7



16 EV11



15



15



CLEANER



1



LYSE



D



D



3H_9005a 1



2



3



4



5



6



7



8



IHM BOARD 50



J3



+2.2V CPU 8



JTAG



COLDFIRE MCF5272 32bit 66MHZ CPU



DC



-12V



DC



EN. HT



+12V -12V +5V



1MBYTE x 16 SDRAM DC



+60V



4



+12V



EXTENDED KEYBOARD



FSC



J9 +12V -12V



LOGIC & VOLTAGE TRANSLATION 32



23



4Kb SPI EEPROM



J14 START CYCLE SWITCH



CARD ID DIFFERENTIAL PRESSURE SENSOR TO ADC



005-1001-84 06 170 DOOR SWITCH J1



2



005-1001-84 06 370



STEPPER MOTOR DRIVER



LDO REG. +2.5V +5V



4



COUNT SIG.



DC DC



IHM CLK & CTRL SIGNALS



FPGA



SPI BUS



SPI RTC CLOCK



5



ADC DATA & CTRL



DATA BUS



+3.3V



ADDRESS BUS



DC



2



ANALOG CTRL.



1MBYTE x 16 SDRAM DC



CR



TO J13 TO J6



J13



ALL DC FEEDBACK CARD ID



+5V +3.3V



ADC SYNC.



ANALOG



+60V



SPI BUS J6 +12V ALL -12V



LCD CONTROLLER



ADDRESS, DATA & CRTL BUS



J7



4



+3.3V +5V



DC



1MBYTE x 16 FLASH



POWER SEQUENCER



POWER SUPPLY AND CONTROL



LDO REG.



28



+24V



4



+3.3V



BDM



ANALOG INPUTS ENCLOSURE TEMP



+12V -12V +5V



J8 26



THERMOSTATED ENCLOSURE J2



POWER SUPPLY AND CONTROL



CPU BOARD



TO J14 TO J9 AXIAL LIGHT LOSS PREAMPLIFIER BOARD



005-1001-84 05 100



+24V +5V



J4 4



005-1001-80 01 220



11



J5



4



OPTICAL BENCH



005-1001-82 880 250



STEPPER MOTOR DRIVER +24V +5V



TO J29



ROCKER MOTOR



OPTICAL BENCH LED



HOME ROCKER FORWARD SCATTER PREAMPLIFIER BOARD



J500 4



005-1001-80 01 220



4



005-1001-82 880 300



8



J501



NEEDLE MOTOR TO J9



Auto. PWR. ON STEPPER MOTOR DRIVER +24V +5V



J10 4



005-1001-80 01 220



4



005-1001-82 880 200



11



SERIAL 1 & 2



J11



HOME NEEDLE



ON/OFF SWITCH



PREAMPLI BOARD



SYRINGE MOTOR



VALVE DRIVER SPI POWER DRIVER 16 S 005-1001-84 03



HOME SYRINGE 6 VALVES



SPI POWER DRIVER 8 S J15



4



8



005-1001-84 04



SPI_CS_EV17-22



4



+24V EV



10



5 VALVES



J21



HEATING FUSE



4A-T



MAIN FUSE 4A-T



J650 +24V SUPERLED_PWR 2



OPTICAL FB CTRL LED CURRENT CTRL



+5V



DC



7



+24V EV



14



005-1001-84 04 DC



7 VALVES



J28 6



12



+24V EV



2



PROTECTED DUAL MOSFET



0.2A POLYSWITCH



J20



J19



J18



J17



J22



J23



005-1001-84 01 120



5



005-1001-84 01 120



25



12



SPI_CS_EV1-16



+5V +3.3V



+5V +3.3V



+24V_DC_HEAT



6



005-1001-83 550 105



USB CONTROLLER



J12



005-1001-82 04 150



SPI BUS +24V +5V



ETHERNET PHY



005-1001-85 01 50



PRINTER CONTROLLER



ENCLOSURE °C SENSOR 0.5°C J29



J26



TUBING SPRING SHIELD



HEATING MODULE MINI-DIN 4 POSITION POWER JACK



DB25-F PARALLEL PORT



DB9-F SERIAL PORT



DB9-M +5V/200mA POWERED SERIAL PORT



2



10/100 BASE-T USB USB ETHERNET TYPE A TYPE A PORT PORT 1 PORT 2 3



ENCLOSURE HEATER



AMBIANT °C SENSOR 0.5°C INTERNAL FAN



WBC



RBC



2



TUBING SPRING SHIELD



HEATING FAN 2



COUNTING CHAMBERS 2



THERMOSTAT OPENS AT 70 +/-5°C REAGENT HEATER



3



REAGENT °C SENSOR 0.5°C



ABBOTT 22 ELECTRICAL SYNOPTIC DRAFT 0.2 12/06/2008



PARTS LIST PARTS LIST     



Two years spare parts kit Maintenance kit O-rings kit Tubing kit Spare parts list



Optical Bench



Optical Manifold



Heating Module



Optical Manifold Tubing and Valves Position



CELL-DYN Emerald Service and Support Manual (Version 202661-106) • © 2007, 2016 • CELL-DYN, CELL-DYN Emerald and CELLDYN Emerald 22 are trademarks of Abbott Laboratories in various jurisdictions. • Abbott Park, IL 60064 USA • All rights reserved.



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3H_8028h.jpg[4/4/2016 10:23:54 AM]



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3H_8027d.jpg[4/4/2016 10:24:20 AM]



Software



Software Note Refer to the CELL-DYN Emerald System Operations Manual for explanation of the software operation.



Emerald Analyzer USB Key Data Format This section describes the use of a USB key for storing and writing automaton data. Security Specifications To avoid the exchange of private data between different analyzers via USB, such specific files contain the serial numbers of analyzer and CRC. So, verification can be made before upload on data analyzer. The data could only be uploaded onto the right analyzer. Interface Specification The file system type is FAT16. The file and folder names are limited to 8 characters +3 for the file extension. The content files are ASCII and some fields are coded in UTFB: SID, PID, ID, Blood Type, operator, target name. Root Folder The USB key can contain data for multiple automaton. The limit is its capacity. At the root folder level of the USB key, a folder contains all Emerald data type: AB18. Automaton ID Folder Each automaton data group is stored on the USB key inside a unique sub-folder: xxxxxx\yyyyyy = 12 digit Emerald serial number. Inside this sub-folder, the data is private. An automaton can only access its own private and all common data. Private data exchange between two automatons is impossible. USB Key Folder Overview The analyzer serial number is coded as xxxxxx-yyyyyy (MSB-LSB). The root folder (AB18) contains only Emerald type analyzer data only, USB Key Structure shows the file structure of the USB key: USB Key Structure



USB Key Data Files Overview



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3105.htm[4/4/2016 10:21:12 AM]



Software



The USB key contains both PRIVATE and COMMON data. PRIVATE Data Inside AB18\XXXXXX\YYYYYY Folder



Folder



File Name



Contents



Comments



Format, unit



Analyzer CRC Read/Write Control



CALI



221OGb.CAL (calibration date DDMMYY)



Calibration report = calibration factors + target + results



One file per calibration date



Ascii + UTF-8 current unit



YES/NO



NO



LOGS



LOG_ERRCSV LOG.CSV



Error + Service Logs



Two files



Ascii



YES/NO



NO



QC



CRXV6875QCN (target name. level)



QC report = target + One file per QC target and level results



Ascii + UTF-8 STD unit



YES/YES



YES



EQC



ID.DAT LOWQCDAT NORMQCDAT HIQCDAT



ID file + EQC results



One sub folder per target. One file per level + ID file



Ascii ABBOTT format STD (USA)



YES/NO



NO



RESULTS



0047CSV (sequence number CSV)



Result analysis



One sub folder per Year/month (YYYY-MM), One sub folder per day (DD), One file per result Limited to 510 files



Ascii + UTF·8 current unit



YES/YES



YES



SETTINGS



TECH.SET



Private analyzer settinqs



One file



Ascii + UTF·8



YES/YES



YES



Private reagents log



One file



Ascii



YES/YES



YES



REAGENTS REAGLOGS.CSV COMMON Data Inside ABl8 Folder



Folder



File Name



Contents



Comments



Format, unit



Analyzer Read/Write



CRC Control



LOTS



MXC1234.QCN MXBC02174.QCL (target name.level) CAL0233.CAL (target name.CAL)



QC target values and Calibration target values



One file per target, limited to 100 files



Ascii ABBOTT format STD (USA)



NO/YES



NO



SETTINGS



USER.SET



Common settinqs



One file



Ascii + UTF·8



YES/YES



YES



UPDATE\VERSION V100-004.b (version release software name.b)



Software release



One file per software release



binary



NO/YES



YES



UPDATE\PRVDRV



Printer drivers



One file per printer driver



binary



NO/YES



YES



HP6122.LPT (prn_name.port LPT or USB)



Specific Technician Display This section describes the software operations which are only accessible with the service password. User Access Menus



Links Emerald Software Tree 3H_8100.PDF Service Password The service password is: AFSE. Note It is strictly forbidden to disclose the password to the customer. The service displays are accessible from MAIN SCREEN then MAINTENANCE then ABBOTT SERVICE.



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3105.htm[4/4/2016 10:21:12 AM]



Software



Service Screen The following activities are available in the service screen: COUNTING PARAMETERS - adjustment of the counting parameters and lyse quantity. These adjustments are possible with the Factory Access Level only. APERTURE CURRENT OFF - test the aperture current. SET GAINS - latex adjustment and HGB blank adjustment. PARK SYRINGE - to park pistons prior to transport. SET SERIAL NUMBER - enter analyzer serial number. LOG. INTERVENTION - fill up the log accessible from the MAIN MENU. Set Gains Screen The following activities are available in the set gains screen: BURN-IN - Not available: manufacturing parameters. ADJUST HGB - adjust the HGB blank level. WBC GAIN - adjust the WBC gain with the use of special latex particles. RBC PLT GAIN - adjust the RBC and PLT gain with the use of special latex particles. AUTO (check box) - when checked, adjustments are automatically performed.



CELL-DYN Emerald 22 Software



Software Exchange Interface Specifications



Folders Overview on the USB Key The analyzer serial number is coded as xxxxxx-yyyyyy (MSB-LSB).



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3105.htm[4/4/2016 10:21:12 AM]



Software



Data Files Overview on the USB Key



PRIVATE DATA in AB22\xxxxxx\yyyyyy Folder



Calibration report = calibration factors + target + results Error + service logs QC report = target + results



One file per calibration date



Ascii + UTF-8 current unit



 



CRC Control  



NO/YES



NO



Two files



Ascii



NO/YES



NO



One file per QC target and level



Ascii + UTF-8 STD unit



YES/YES



YES



ID.DAT LOWQC.DAT NORMQC.DAT HIQC.DAT



ID file + EQC results



Ascii ABBOTT format STD (USA) unit



 



 



NO/YES



NO



0047.CSV (sequence number.CSV)



Result analysis



 



 



YES/YES



YES



TECH.SET



analyser settings



One sub folder per target = lot name , 1 file per level + ID file One sub folder per Year/month (YYYY- MM), One sub folder per day (DD), One file per result Limited to 510 files One file



YES/YES



YES



USER.SET



User settings



One file



Ascii + UTF-8 Ascii + UTF-8



YES/YES



YES



REAGLOGS.CSV 0047.CSV (sequence number.CSV)



Private reagents log Result analysis with Raw information.



One file One sub folder per Year/month (YYYY- MM), One sub folder per day (DD), One file per result Limited to 510 files



Ascii Ascii + UTF-8 current unit



YES/YES  



YES  



NO/YES



YES



Files Name (example) 221006.CAL (calibration date DDMMYY)



Folder CALI



LOG_ERR.CSV LOG.CSV CRXV6875.QCN (target name.level)



LOGS QC



EQC



RESULTS



SETTINGS



REAGENTS RAW



Contents



Stored under specific conditions.



Comments



Format, Unit



Ascii + UTF-8 current unit



Analyzer Read/Write



COMMON DATA in AB22 Folder



Folder



Files



Contents



Comments



CRC Format, Analyzer Read/Write Control Unit Ascii   ABBOTT YES/NO format NO USA (STD)unit



Qc target values & Calibration target values



One file per target Limited at 100 files



UPDATE\ V100-004.b VERSION ([version name].b)



Software release



One file per software release



binary



UPDATE\ HP6122.LPT PRTDRV (prn_name.port LPT or USB )



Printer drivers



One file per printer driver



binary



LOTS



MXC1234.QCN MBC02174.QCL (target name.level) CAL0233.CAL (target name.CAL)



 



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3105.htm[4/4/2016 10:21:12 AM]



YES/NO YES/NO



YES YES



Software



CELL-DYN Emerald Service and Support Manual (Version 202661-106) • © 2007, 2016 • CELL-DYN, CELL-DYN Emerald and CELL-DYN Emerald 22 are trademarks of Abbott Laboratories in various jurisdictions. • Abbott Park, IL 60064 USA • All rights reserved.



https://gssweb.abbott.com/WWCS/hematology/emerald_html/3105.htm[4/4/2016 10:21:12 AM]



Version - 202666-102_4364_4



A1.01 Sampling Module List/Part Numbers List/Part Number



Description



8701688101



Sampling module



Inspect tools for damage, ensure calibration is not expired and replace if necessary.



A1.01 Sampling Module



Time Required 1 hr. Tools/Materials



Adjustable Torque Driver TORX T10 Tool



While performing this procedure, if you are exposed to reagents, calibrators or controls, the following statements may apply: WARNING Potential Biohazard Caution Chemical Hazard



Removal Action



Steps



Prerequisite 1. Perform DRAIN ALL (MAIN MENU,



MAINTENANCE, FLUIDICS, DRAIN ALL).



2. Power OFF the instrument and disconnect the



power supply.



3. Open the door on the right side of the



instrument (hydraulic area access).



Remove Upper Cover



1. Loosen the three (3) screws securing the upper



cover to the front and back of the instrument and remove the cover. 2. Remove the sampling probe.



Reference



Remove Sampling Module



1. Loosen (do not remove) the screw securing the



sampling module. [1]



2. Remove the screw securing the probe optical



sensor. [2]



3. Disconnect the two (2) tubes [4, 5] and cut the



tie wraps (as needed).



4. Loosen (do not remove) the two (2) guide



screws. [3]



5. Slide the guide downward. 6. Remove the sampling module.



Replacement Action Install Sampling Module



Steps



1. Install the sampling module onto the guide. Note There should be some space between the module and guide to prevent the two from binding 2. Push the guide up to make contact with the sampling module. 3. Tighten the two (2) guide screws [3] to 125cNm (11.06lb-in). 4. Connect the tubing [4, 5] and attach new tie wraps (as needed). 5. Install the screw [2] securing the probe optical sensor. Tighten screw to 80cNm (7.08lb-in). 6. Tighten the screw [1] To 15cNm (1.32lb-in) securing



Reference



the sampling module.



Install Sampling Probe Install Upper Cover



1. Install the sampling probe.



1. Install the upper cover to the front and back of the



unit using three (3) screws.



Power ON Instrument



1. Connect the instrument to the power supply and



power ON the instrument.



Verification Procedures Order



VP Description



VP Detail / Note



1



G23 - Initialization/ Startup



Verify instrument initializes and/or starts up.



N/A



  G110 - After repair is complete, verify per released Operation and Service Procedures. If the system/instrument produces results, ENSURE appropriate Quality Control is in specification and calibrate as necessary.



Expected Results



CELL-DYN Emerald Service and Support Manual (Version 202661-102) • © 2007, 2016 • CELL-DYN, CELL-DYN Emerald and CELL-DYN Emerald 22 are trademarks of Abbott Laboratories in various jurisdictions. • Abbott Park, IL 60064 USA • All rights reserved.



Version - 202666-102_4351_



B1.01 Counting Module: Counting Chamber List/Part Numbers List/Part Number



Description



8701685902



Counting Chambers W/O aperture



8701685903



Counting Chambers W/O aperture



Inspect tools for damage, ensure calibration is not expired and replace if necessary.



B1.01 Counting Module: Counting Chamber



Time Required 1 hr. Tools/Materials



Standard Tool Kit TORX T10 Tool



While performing this procedure, if you are exposed to reagents, calibrators or controls, the following statements may apply: WARNING Potential Biohazard Caution Chemical Hazard



Removal Action



Steps



Preparation 1. Perform DRAIN ALL (MAIN MENU, MAINTENANCE,



FLUIDICS, DRAIN ALL).



2. Power OFF the instrument and disconnect the power



supply.



3. Open the door on the right side of the instrument



(hydraulic area access).



Remove Counting Heads



1. Remove the screws and disconnect the GND cables



from the WBC and RBC counting heads. [1]



2. Remove the counting heads.



Note The right (RBC) counting head is labeled 5. The left (WBC) counting head is labeled 8. They are



Reference



not interchangeable. Note Move each counting head one quarter of a turn counter clockwise (CCW) to remove it.



Remove Counting Chamber



1. Disconnect the HGB, RBC, and WBC cables from the



Preamplifier Board (PAM). [3]



2. Remove the lyse tubing from the counting chamber. [4] 3. Pull gently on the clip, while pushing the top of the



counting chamber toward the inside of the instrument. [1] 4. Raise the counting chamber and remove it. [2].



Replacement Action Install Counting Chamber



Steps



1. Lower the counting chamber into position. [2]



Note Ensure that the counting chamber is fitted with a set of O-rings. The O-rings should be greased before



Reference



installation onto the counting chamber. 2. Push on the top of the counting chamber, securing the clip



into the proper position. [1]



3. Connect the HGB, RBC, and WBC cables to the PAM. [3] 4. Connect the lyse tubing to the counting chamber. [4]



Install Counting Heads



1. Install the counting heads.



Note The right (RBC) counting head is labeled 5. The left (WBC) counting head is labeled 8. They are not interchangeable. Note Move each counting head one quarter of a turn clockwise (CW) to install it. 2. Connect the GND cables [1] to the WBC and RBC counting



heads and tighten the screws.



Power ON Instrument



1. Connect the power supply and power ON the instrument.



Verification Procedures Order



VP Description



VP Detail / Note



3



G6 - Calibration



Verify calibration per operator's manual.



Expected Results



1



G23 - Initialization/ Startup



2



VP-03 Electronics Gain Adjustment



N/A



  G110 - After repair is complete, verify per released Operation and Service Procedures. If the system/instrument produces results, ENSURE appropriate Quality Control is in specification and calibrate as necessary.



Verify instrument initializes and/or starts up.



CELL-DYN Emerald Service and Support Manual (Version 202661-102) • © 2007‡&(//'