SOP of INSTRUMENTS REV [PDF]

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STANDARD OPERATING PROCEDURE



NTPC Korba



Conductivity measurement of a Solution Instrument: JENCO conductivity meter



Model: 3173 COND.



Step 1: Switch ON the main switch in switch board Step 2: Press the ON/OFF switch of the instrument Step 3: It will show Cell K value, Battery level and Temperature Step 4: It will go to ‘AUTORANGING’ mode and show ‘rAn6’ Step 5: Then it will show the conductivity of the solution in the beaker in which the sensor is dipped (generally DM water) Step 6: Wash the beaker and pour the sample solution in it to measure its conductivity (ensure the sensor is dipped in the soln.) Step 7: The conductivity will be displayed on the screen Step 8: Press ‘MODE’ to get the values of Salinity and TDS of the soln. Step 9: Wash the beaker, fill it with DM water and dip the sensor in it Step 10: Press the ON/OFF switch of the instrument to turn it OFF Step 11: Switch OFF the main switch in switch board



STANDARD OPERATING PROCEDURE



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Calibration of instrument Instrument: JENCO conductivity meter



Model: 3173 COND.



Step 1: Prepare std. soln. of 0.01N KCl (cond. 1413µs/cm) or 0.001N KCl (cond. 141.3µs/cm) for calibration Step 2: Switch ON the instrument and wait for its stabilization Step 3: Press ‘CAL’ key for calibration Step 4: It will show previously set value of temp. coeff. (1.91%) with ‘CAL’ showing on the top. To change it use ‘up key’ (∆) or ‘down key’ and save it by pressing ‘ENTER’ Step 5: Press ‘MODE’ to exit and go to next calibration parameter Step 6: Similarly it will show Temp. (25 deg C)(with ‘CAL’ showing on the top) and Cell K (0.100) (with ‘CELL K=’ showing on the top) values. To change it press ‘up’ or ‘down’ keys and press ‘ENTER’ to save or press ‘MODE’ to exit Step 7: Then immerse the probe in std soln and it will show the conductivity of the soln (with ‘AUTO RANGING’ showing on the top). Step 8: To calibrate cell constant press ‘up’ or ‘down’ keys to match the cond. value with the std soln and press ‘ENTER’ to save or press ‘MODE’ to exit Step 9: It will now display the conductivity of std soln with ‘CELL K=’ flashing on screen Step 10: To calibrate conductivity, press ‘up’ or ‘down’ keys to match the cond. value with the std. soln. (it will display cond value with ‘CELL K=’ staying on) Step 11: After matching the cond. value with std. soln., ‘CELL K=’ will start flashing again Step 12: Press ‘ENTER’ to save the value and then press ‘MODE’ to exit calibration and return to normal operation



STANDARD OPERATING PROCEDURE



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Absorbance measurement of a Solution Instrument: SHIMADZU UV-Vis Spectrophotometer



Model: UVmini 1240



Step 1: Switch ON the main switch and then the POWER switch of the instrument Step 2: It will show ‘Initialize’ and wait for 5 minutes Step 3: Then it will go to ‘Mode Menu’ Step 4: Press ‘F1’ to measure Parameters. It will open the ‘Parameter file list’ Step 5: Press ‘F2’ to set the Serial No. of the parameter to be measured Step 6: It will ask to ‘Enter file no.’ and write the serial no. using number keys and press ‘ENTER’ (If want to go back to previous setting window, press ‘RETURN’) Step 7: It will open ‘Quantification’ window where it will give the information about wavelength, multipoint calibration std. no. etc. Step 8: Press ‘F3’ for Sample Measurement (SmplMeas) Step 9: Take the blank solution in the cuvette (50 mm cell) and place it in the chamber maintaining the correct position of the cuvette. Step 10: Press ‘Auto Zero’ and note the absorbance is showing 0 Step 11: Take out the cuvette, wash with DM water and take sample in it (after rinsing). Place the cuvette in the chamber, press ‘START’ and note down the reading Step 12: Press ‘RETURN’ to go to ‘Mode Menu’ (Step 3) and repeat if needed Step 13: Take out the cuvette after experiment is over and wash it with DM water Step 14: Switch OFF the POWER switch of the instrument and then the main switch



STANDARD OPERATING PROCEDURE



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Measurement of %O2 Instrument: GASALERT O2



Model: GAXT-X-DL-2



Step 1: Long press the ‘Green button’ to switch ON the instrument and wait for its initialization Step 2: If it is showing ‘PAST’ in the display then press the ‘Purple button’ (extreme right side) Step 3: Insert the pipe in the confined space. Step 4: Take suction by pumping (4-5 times) while covering the open portion of the Black hemispherical part (to which the rubber tube is connected to the instrument) Step 5: After the instrument vibrates with alarming sound, stop suction and the reading of O2 purity will be displayed after stabilization Step 6: Long press the ‘Green button’ to switch OFF the instrument



STANDARD OPERATING PROCEDURE



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Conductivity measurement of a Solution Instrument: KENT Conductivity meter



Model: EIL5007



Step 1: Switch ON the main switch in the switch board Step 2: Set the upper limit of conductivity of the sample to be analysed by using the knob (extreme left side). [It can be set as 1/3/10/30/100/300/1000] Step 3: Pour the sample in the sample cell & put the sensor in it Step 4: Read the conductivity of the sample by the deflection of the moving stick of the conductivity meter  If the upper limit is set as 1/10/100/1000 then the upper scale (0 to 10) is considered to read the value accordingly  If the upper limit is set as 3/30/300 then the lower scale (0 to 3) is considered to read the value accordingly Step 5: Wash the sample cell with DM water and keep the sensor dipped in DM water taken in the sample cell Step 6: Rotate the knob anticlockwise to go back to its original position Step 7: Switch OFF the main switch



STANDARD OPERATING PROCEDURE



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Measurement of %CO2 & %O2 of a gas sample: Instrument: ORSAT Step 1: Connect the clamped bladder to the suction pipe of ORSAT & keep the soln. bottle on the table [ensure all knobs are in closed condition] Step 2: Open the clamp of the bladder to release the sample Step 3: Rotate the 1st knob 90˚ clockwise (keeping the mark on the knob as



)



Step 4: When air comes out of soln. bottle, place the bottle on the top of ORSAT and keep it like this for 10-15 sec Step 5: Rotate the 1st knob 90˚ clockwise (



) and soln. starts rising through the



graduated tube Step 6: As soon as the soln. level reaches the mark 100 inside the graduated tube, rotate the 1st knob 90˚ anticlockwise ( ) Step 7: The soln. level goes down in the graduated tube and as it reaches near the mark 0, rotate the knob 90˚ anticlockwise (



)



Step 8: Move the soln. bottle downwards. As the soln. level reaches the mark 0 inside the graduated tube, press the pipe connecting the graduated tube and the soln. bottle and rotate the 1st knob 360˚ for 2 times (



)



Step 9: Match the soln. level of the graduated tube with the soln. bottle level. Both levels should match with the mark 0 (otherwise repeat step 8 & 9). Clamp the bladder.



STANDARD OPERATING PROCEDURE



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Step 10: Rotate the 2nd knob 90˚ to allow the sample into KOH solution Step 11: Move the soln. bottle upwards and downwards for 8-10 times [KOH soln. level also goes down and up. Ensure the KOH soln. level does not go above the 2 nd knob] Step 12: Set KOH soln. level upto the mark on that tube by moving the soln. bottle accordingly & rotating the 2nd knob 90˚ to stop air flow through it Step 13: Now match the soln. levels of the graduated tube & soln. bottle and note the reading (a1). It gives %CO2 of the sample Step 14: Rotate the 3rd knob 90˚ to allow the sample into Pyrogallol soln. Step 15: Follow the step no. 11 & 12 for the Pyrogallol soln. also Step 16: Now match the soln. levels of the graduated tube & soln. bottle and note the reading (a2). (a2 - a1) gives %O2 & (%O2 x 5) gives %air ingress in H2 sample Step 17: Release the sample by rotating the 1st knob 180˚ clockwise (



)



Step 18: Rotate the 1st knob 180˚ clockwise to close the air path through the tube (



)



STANDARD OPERATING PROCEDURE



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Dissolved O2 measurement of a solution Instrument: HACH ORBISPHERE DO meter



Step 1: Connect one of the pipes of the instrument to the sampling point and the other pipe will be used as discharge Step 2: Press the Power switch on the instrument to switch it ON Step 3: Display will show the DO value. Allow it to stabilize Step 4: Note down the DO value Step 5: Press the Power switch on the instrument to switch it OFF



STANDARD OPERATING PROCEDURE



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Dew Point measurement of a gas sample Instrument: Alphamoisture dewSMART portable Dew Point meter Model: DSP-Ex Step 1: Place the instrument close to sampling point and move the handle downwards from its holding position by pressing side buttons (both side) Step 2: Switch ON the instrument by pressing the button marked I/0 Step 3: The display will read INITIALISING > INSTRUMENT OK > CALIBRATION DATA OK > ˚C Dewpoint of air surrounding the sensor Step 5: Connect the connector of the sampling tube with the instrument connector point by pressing it Step 6: Connect the other end of sampling tube (rubber tube end) with the sampling point Step 7: Open the sample v/v and maintain air flow of 5-10 L/min through the instrument (check it by sensing the air coming out of the sample exhaust of the instrument) Step 8: Block the sample exhaust by pressing finger on it and the measuring head of the instrument will come up (ensure it has fully come up otherwise increase air flow by controlling sample v/v) Step 9: Remove finger from the sample exhaust and press the measuring head back (Repeat it for 2 times) Step 10: Again block the sample exhaust. When the measuring head of the instrument comes up fully, it will show ˚C Dewpoint of the sample air Step 11: Press the measuring head back to close it completely Step 12: Press the button I/0 to switch OFF the instrument Step 13: Disconnect the sampling tube by pressing it