![Photometer 5010 V5+ Service Manual [PDF]](https://pdfs.asia/img/200x200/photometer-5010-v5-service-manual.jpg)
10 0 14 MB
Photometer 5010 V5+ Version 5.x > SN 7000
Service Manual
CONTENTS chapter
headline
1
General notes
1.1
Contact partner
1.1
Sm_V5_a.pdf
1.2
Proved security
1.1
Sm_V5_a.pdf
1.3
Precautions and hazards
1.2
Sm_V5_a.pdf
2
Service concepts
2.1
Service level
2.1
Sm_V5_a.pdf
2.2
Conditions
2.2
Sm_V5_a.pdf
3.1
Block diagram
3.1.1
Sm_V5_a.pdf
3.2
Short instruction guide
3.2.1
Sm_V5_a.pdf
3.3
Service tools
3.3.1
Sm_V5_a.pdf
3.4
Plug identification
3.4.1
Sm_V5_a.pdf
4.1
Mains power circuit
4.1.1
Sm_V5_b.pdf
4.2
Mains connector
4.2.1
Sm_V5_b.pdf
4.3
AC / DC power supply
4.3.1
Sm_V5_b.pdf
4.10
Optical unit
4.10.1
Sm_V5_b.pdf
4.11
Filter wheel
4.11.1
Sm_V5_b.pdf
4.12
Halogen lamp
4.12.1
Sm_V5_b.pdf
4.13
Unit Photodiode
4.13.1
Sm_V5_b.pdf
4.14
Unit cuvette adaptor
4.14.1
Sm_V5_b.pdf
4.15
Unit Peltier element
4.15.1
Sm_V5_c.pdf
4.16
Step motor pump
4.16.1
Sm_V5_c.pdf
4.18
Step motor filter wheel
4.18.1
Sm_V5_c.pdf
4.19
Light barrier filter wheel
4.19.1
Sm_V5_c.pdf
4.20
Bubble detector with plug
4.20
Sm_V5_c.pdf
4.21
Flow-through cuvette adaptor
4.21
Sm_V5_c.pdf
4.22
Standard cuvette adaptor
4.22
Sm_V5_c.pdf
4.23
Connector bubble detector
4.23
Sm_V5_c.pdf
4.24
Lamp holder
4.24
Sm_V5_c.pdf
4.26
Tubes
4.26.1
Sm_V5_c.pdf
RIELE BERLIN
Photometer 5010 V5+
page
file Sm_V5_a.pdf
Sm_V5_a.pdf
1
11.04.2007
CONTENTS chapter
headline
page
file
4.30
Top case
4.30.1
Sm_V5_c.pdf
4.31
Top case pre-assembled
4.31.1
Sm_V5_c.pdf
4.32
Metal tube inlet
4.32.1
Sm_V5_c.pdf
4.33
Microswitch with plug
4.33.1
Sm_V5_c.pdf
4.34
Plastic foil touchscreen
4.34.1
Sm_V5_c.pdf
4.35
Printer mechanism 8NAD
4.35.1
Sm_V5_c.pdf
4.40
PCB RR-120 Mother board
Sm_V5_d.pdf
Function description
4.40.1
Sm_V5_d.pdf
Component diagram
4.40.2
Sm_V5_d.pdf
Voltages
4.40.3
Sm_V5_d.pdf
Voltage 12 VDC of halogen lamp
4.40.4
Sm_V5_d.pdf
Fuses F1, F2 and F3
4.40.4
Sm_V5_d.pdf
Amplifier for photocurrent and ADC
4.40.5
Sm_V5_d.pdf
Adjustment of amplifier stage
4.40.6
Sm_V5_d.pdf
Temperature control
4.40.8
Sm_V5_d.pdf
Adjustment of temperature control
4.40.9
Sm_V5_d.pdf
Thermal adjustment
4.40.10
Sm_V5_d.pdf
Installation instruction
4.40.11
Sm_V5_d.pdf
Circuit diagrams
4.40.12
Sm_V5_e.pdf
4.41
Touchscreen
4.41.1
Sm_V5_f.pdf
4.42
Touch panel
4.42.1
Sm_V5_f.pdf
4.43
PCB RR-122 Display, printer, touch
4.43.1
Sm_V5_f.pdf
4.45
PCB RR_121 Motor driver
4.45.1
Sm_V5_f.pdf
4.50
Fan
4.50.1
Sm_V5_f.pdf
4.51
Bottom case
4.51.1
Sm_V5_f.pdf
4.52
Carton Photometer 5010
4.52.1
Sm_V5_f.pdf
5
Assembling / Dismantling
6.1
Communication protocol
6.1.1
Sm_V5_i.pdf
6.2
Download of operating system: Resume after malfunction
6.2.1
Sm_V5_i.pdf
6.3
Initialization of bootstrap
6.3.1
Sm_V5_i.pdf
6.4
Configuration of operating system
6.4.1
Sm_V5_i.pdf
7
Trouble shooting
7.1.1
Sm_V5_i.pdf
8
Spare parts
8.1.1
Sm_V5_i.pdf
9.1
Calibration procedure of Photometer 5010 Subject: Cuvette temperature
9.1.1
Sm_V5_i.pdf
RIELE BERLIN
Photometer 5010 V5+
Sm_V5_g/h.pdf
2
11.04.2007
1
GENERAL NOTES The data and information provided in this manual correspond to the state of knowledge existing at the time of introducing the Photometer 5010 V5+ on the market. Any important changes will be taken into account in the next edition of this manual. In any case, the respective packaging leaflet should be regarded as authoritative. This service manual was created for the telephone service and technical service staff.
1.1
Contact Partner Technical product management and support: ROBERT RIELE GmbH & Co KG
1.2
Address:
Kurfuerstenstraße 75 - 79 D - 13467 Berlin Germany
Phone: Fax:
+49 / 30 / 404 40 87 +49 / 30 / 404 05 29
E-mail:
[email protected]
Proved Security This device has EN 61010-2-101.
been
constructed
under
the
regulations
of
EN
61010-1
and
This device was examined and left the factory in perfect technical condition. To preserve this and protect safe and faultless operation, the user must follow the instructions and remarks of this service manual. To preserve this condition and to guarantee a safe working, information and working advices, which are included in these working instructions, have to be heeded by the applier. This device meets protection 1 (earth conductor wire).
1.3
Precaution and Hazards All electrical equipment is potentially hazardous. Never remove covers without first ensuring that it is isolated from the AC supply, unless specific maintenance instructions or repairs are being carried out by authorized ROBERT RIELE personnel.
The hard- and software is subject to a program of continuous evaluation and improvement and, therefore, may be changed in the future. This also concerns service requirements.
All samples and reagents should be treated with caution accorded to those known to contain pathogenic organisms. Similarly, the cleaning of component part of Photometer 5010 V5+ should be carried out in compliance with health and safety.
RIELE BERLIN
Photometer 5010 V5+
1.1
10.05.2006
1
GENERAL NOTES Warning: All components used on Photometer 5010 V5+ must be regarded as potentially dangerously contaminated when doing repair work. Use rubber gloves or double gloves whenever cleaning or sterilizing components. The most frequently used components (tubing, cuvette, measuring chamber flap, sip. lever and waste container) should be cleaned and treated with a suitable disinfecting solution (75% alcohol) prior to doing any service work.
Disinfect and wash hands after work is completed.
This device has been constructed and checked in accordance with standards EN 61010-1 and EN 61010-2-101. When the device leaves our factory it is in perfect condition from the point of view of work safety. To keep it that way and to ensure safe operation the user must follow the instructions and warnings given in the operation instruction manual.
The electrical protection of the device corresponds to Class 1 (it has a protective earth).
The power plug must only be inserted into a socket that has a protective contact. The protection must not be abolished by using an extension lead that does not have a protective earth wire.
Warning: Any break in the earth wire inside or outside the device and any loose connection of the earth wire can make the operation of the device dangerous. A deliberate break or interruption is not allowed.
When the housing is opened or the components are being removed (except when this can be done by hand), live parts may be exposed. Connection may also be live.
Therefore, if the carrying out of an adjustment, service or repair on the open device in the live state is unavoidable, this must be done by an expert who is familiar with the dangers involved.
Make sure you only use fuses of the specified type and rated amperage when replacing the old ones. Repaired fuses must not be used and the fuse holder must not be bypassed.
If you have any reason to believe that the device can no longer operate safely, take it out of use and make sure no one can use it accidentally.
It must be assumed that safe operation is no longer possible when the device: • shows visible signs of damage • fails to operate • has been stored under unfavorable conditions for a fairly long time • has been transported under rough conditions
The Photometer 5010 V5+ should be used by appropriately qualified persons only.
RIELE BERLIN
Photometer 5010 V5+
1.2
10.05.2006
2
SERVICE CONCEPTS
2.1
Service Level From the early stage of development, the Photometer 5010 V5+ was designed for simple error detection and easy exchangeability of modules. This gives the service workshops the possibility of a fast and easy repair of the device on service level A (module level). No big stock or expensive equipment is necessary and service technicians are easier to train. Also, a permanent technical improvement in layout and components took and takes place for better productivity and economic production.
On repairable modules the quality and function is always provided by the manufacturer according to the latest technology. This keeps Photometer 5010 V5+ always on the highest quality level.
The exchange price for modules will be kept on a low level to guarantee repairs, on an economical basis.
2.2
Conditions Warranty period for devices ...
The warranty period for devices is 16 months starting with the date of shipment ex works Berlin/Federal Republic of Germany or 12 months starting with the date of the first installation, whichever period is shorter.
... and spare parts
For spare parts a warranty of 6 months from installation date of the part applies. Prerequisite for this is that the warranty period for spare parts shall not fall short of the warranty period of the device where a spare part has been installed. A further prerequisite is, that this period shall not exceed a period of 24 months starting with the delivery of the spare part ex works Berlin/Federal Republic of Germany. Hint: In case the device has a remaining warranty period of more than 6 months, the parts remain under warranty until the warranty period of the device expires.
Handling of warranty claims
Replacement of parts The defective parts have to be handled via Return Authorization (RA) procedure. Please answer all the questions on the RA form with the greatest care. Especially a detailed fault description is needed or the warranty claim will not be accepted by the manufacturer. Complete devices are not accepted unless this has been agreed with the service department of ROBERT RIELE GmbH & Co KG. Important information: − Only parts marked with "A" in the price list are generally accepted under warranty. − Only return those parts marked with "R" in the spare part list. − All defective parts ( non-"R" and "A" parts ) should be kept on stock for a period of 7 months. In case the manufacturer needs the part for investigation it will be requested from Berlin. − All parts returned to Berlin and not requested by Berlin will be sent back at the expense of the dealer/customer.
RIELE BERLIN
Photometer 5010 V5+
2.1
10.05.2006
2
SERVICE CONCEPTS Exclusion of warranty
The aforementioned warranties do not apply in case of improper use, handling, transportation or storage, faulty installation, repair or maintenance, Chemical influence or contamination as well as damages resulting from that, failure to follow operating instructions, alterations or modifications of devices or parts thereof not authorized or recommended by ROBERT RIELE GmbH & Co KG and resulting damages, normal wear and tear and in case of other circumstances beyond the control of ROBERT RIELE GmbH & Co KG.
Handling of repairs
As a general rule, all device repairs should be carried out by authorized and trained personnel only. Repair of parts marked with "R" Parts which are economically worth repairing are marked with "R" in the spare parts price list. The defective parts have to be handled via Return Authorization procedure. For correct handling of the exchange it is absolutely necessary to return the complete filled in RA form (preferable via electronic mail), giving full details of the defect. Repair of devices Complete devices are not accepted for replacement or repair unless this has been agreed with ROBERT RIELE GmbH & Co KG. Before replacement or repair can take place, the validity of the request must be examined and the question of costs must be settled in a written agreement with ROBERT RIELE GmbH & Co KG.
Terms of delivery
All shipping conditions for spare parts to the dealer/customer are ex works Berlin. Emergency shipments require additional costs to be charged.
Emergency shipment
In case of emergency, a copy of the complete RA form should be sent by fax (+49 / 30 / 404 05 29) or electronic mail (e-mail: [email protected]) to ROBERT RIELE GmbH & Co KG, Berlin marked with
EMERGENCY
If fax was used for Emergency RA the original RA form has to be mailed or sent by electronic mail to Berlin.
Handling of costs
RIELE BERLIN
Replacements for R-parts are shipped at a repair price. In case the parts are no longer repairable, the dealer/customer will be charged later on with the difference of the new price. Replacements for warranty requests are shipped free of charge. In case the manufacturer does not accept the warranty request, the dealer/customer will be charged later on with the R-price for R-parts and the new price for non R-parts.
Photometer 5010 V5+
2.2
10.05.2006
2
SERVICE CONCEPTS RA form (see form at next page)
Return Authorization Please answer all the questions on the RA form with the greatest care and sign the form. − − − − − − − − − −
Date (problem date) Type of device Serial No. (of the device) Installation date (of device) mark Spare Part (devices should not be returned) Mat.-No. of the spare part Fault description (incl. error code and job codes) mark request e.g. Repair and Replacement fill in RA-number give place and date (when filled in form) and sign RA form Note:
In case of the device is out of warranty
RA sent via electronic mail to Berlin is valid without a signature.
− Installation date of spare part when part was installed within the last 6 months.
All returned parts should be individually labeled with the corresponding RA No. and shipped together with the completed RA form (if not sent via electronic mail) to: ROBERT RIELE GmbH & Co KG Kurfürstenstraße 75 − 79 D-13467 Berlin Germany
RIELE BERLIN
Photometer 5010 V5+
2.3
10.05.2006
3.1
BLOCK DIAGRAM
RIELE BERLIN
Photometer 5010 V5+
3.1.1
11.05.2006
3.2
SHORT INSTRUCTION GUIDE
Utility programs M A I N
M E N U
M E A S U R E
W I T H
P R O G R .
M E A S U R E
W I T H
B A S I C
M E A S U R E
N E W
/
/
0 4 . 1 1 . 0 6
C O P Y L F 1 1 : 3 9
P A G E
A D J U S T M E N T
1 / 5 P A G E
M U L T I - S T A N D A R D P R I N T E R P U M P
/
B U B B L E
3 7 . 0 1 c
D E T E C T
0 4 . 1 1 . 0 6
U T I L I T I E S
A D C
3 7 . 0 1 c
RIELE BERLIN
5 / 5 P A G E
C O U N T S
S E R V I C E
E X I T
Page 1 of utility programs: Scrolling through all utility programs is possible by [PAGE]. The current page is shown at the right upper screen corner. By [EXIT] the program returns to the main menu. A utility program is selected by pressing the relating key.
1 3 : 0 5
P A G E
L A N G U A G E
Utility programs are necessary for the adjustment and maintenance of the photometer.
M E T H O D S
L A M P
U T I L I T I E S D A R K
M E T H O D S
C H A N G E
U T I L I T I E S 3 7 . 0 5 C
Main menu:
Page 5 of utility programs: The last key is representing the service tools.
( O P T I C )
T O O L S
E X I T
0 4 . 1 1 . 0 6
1 3 : 0 5
Photometer 5010 V5+
3.2.1
11.04.2006
3.2
SHORT INSTRUCTION GUIDE
SERVICE
The service tools are reserved for trained specialists only and therefore protected by a password.
TOOLS
PASSWORD
The password is given as
1
2
3
4
5
6
ESC
9
0
.
+
-
7
8 E
Utilities
RIELE BERLIN
13467 Stop function by [ESC].
Description in chapter of OM
Dark level adjustment
7.2.1
Multi-standard function
7.2.2
Printer ON / OFF
7.2.3
Pump / bubble detector
7.2.4
Serial interface
7.2.5
Qualitiy control
7.2.6
Printing settings
7.2.7
Data logging
7.2.8
Tempering ON / OFF
7.2.9
Temperature adjustment
7.2.10
Laboratory name
7.2.11
User name
7.2.12
Error list
7.2.13
Key signal ON / OFF
7.2.14
Touchscreen
7.2.15
Date / Time
7.2.16
Language
7.2.17
ADC counts (Optic)
7.2.18
Service
7.2.19
Photometer 5010 V5+
3.2.2
11.04.2006
3.3
SERVICE TOOLS
SELECTION OF SERVICE TOOLS U T I L I T I E S
P A G E
L A N G U A G E A D C
5 / 5
P A G E
C O U N T S
( O P T I C )
Within the service program level there are functions influencing special basic parameters of the device. Because they are allowed to be changed divergent from the work configuration only in exceptions and only by trained staff, their unintentional selection is protected by a password.
Change in service program with [SERVICE TOOLS]. S E R V I C E
T O O L S
E X I T
Enter password:
[13467]
SERVICE TOOLS PASSWORD
Confirm with [E]. 1
2
3
4
5
6
ESC
9
0
.
+
-
RIELE BERLIN
7
8
The service level will be opened until to the next shutdown of the device.
E
Photometer 5010 V5+
3.3.1
11.04.2007
3.3
SERVICE TOOLS
S E R V I C E S Y S T E M
T O O L S
P A G E
I N I T I A L I S A T I O N
1 / 5 P A G E
M E M O R Y D I S P L A Y
The current page is shown on the right upper corner of the display. Abort of service tool selection and change back to program selection with [EXIT].
T E S T
L A M P
Selection of a desired service tool is possible with [PAGE].
E X I T
A service tool will be invoked by pushing the corresponding button.
Item no. Service tool 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
RIELE BERLIN
SYSTEM INITIALISATION MEMORY DISPLAY TEST LAMP SERIAL NO. PCB LAYOUT SET WAVELENGTH TEST FILTER WHEEL TEMPERATURE ON/OFF TEMPERATURE VALUE CORRECTION VALUE TEMPERATURE CALIBRATION ADC CORRECTION ADC COUNTS (OPTIC) ADC COUNTS (DARK ADJ.) BOOST WAVELENGTH CALIBRATION 12V ADC DISTRIBUTOR TEXT INIT AUTOMATIC RESULTS BCC OF METHOD / CURVE
Photometer 5010 V5+
3.3.2
Description in chapter S1 S2 S3 S4 S5 S6 S7 S8 S9 S10 S11 S12 S13 S14 S15 S16 S17 S18 S19 S20
11.04.2007
3.3
SERVICE TOOLS
DESCRIPTION OF SERVICE TOOLS S1
System Initialisation With [START] the default parameters will be set. S Y S T E M
I N I T I A L I S A T I O N
E X I T
Several settings (see table) are then typed over in the battery powered RAM. They remain active, even after switching the device off/on, until they are specifically changed.
S T A R T
After a print-out of the program release the date and time can be set (chapter OM 7.2.16). DATE mm.dd.yy 10.12.05
TIME hh:mm:ss 14:54:34
1
2
3
4
5
6
ESC
9
0
.
+
-
7
The action ends with a long beep.
8 E
Beeper
ON
Printer internal
ON
Printer external
OFF
Real time clock
ON
Boost mode
up to 390 nm
Language
english
Analog-digital converter correction
150
Temperature control: Correction values
3000
EDP
OFF
EDP protocol
STX / ETX / BCC
Resulting factors of methods based on standard
cleared
Error list
cleared
Laboratory name
not affected
User name
not affected
Data logging
not affected
Quality control
not affected
Multi-standard functions
not affected
Bubble detector
RIELE BERLIN
Photometer 5010 V5+
ON
3.3.3
11.04.2007
3.3 S2
SERVICE TOOLS Memory
S E R V I C E D E L E T E
T O O L S
F R E E
M U L T I - S T D
M E N U
The submenu shows different kinds of memory.
M E T H O D S
M E M O R Y
D E L .
L O G G E R
D E L .
Q C
S2.1
S U B
M E M O R Y
M E M O R Y
E X I T
Delete Free Methods
D E L E T E
F R E E
M E T H O D S
Attention! With [START] all methods edited by the user are deleted without a call-back. In the case of doubt these methods should be checked before: A print out of all stored methods can be done for an optional later new programming. The action ends with a long beep.
E X I T
S2.2
S T A R T
Multi-Standard Memory Attention! With [DEL] the memory assigned to nonlinear methods is deleted without a call-back. M U L T I - S T D
M E M O R Y
The contents of the memory can be exported and imported by special remote commands (chapter 6.1 – Communication protocol). The action ends with a long beep.
E X I T
RIELE BERLIN
D E L
S E T
Photometer 5010 V5+
3.3.4
11.04.2007
3.3 S2.3
SERVICE TOOLS Delete Logger Memory Attention! With [START] the memory assigned to stored results is deleted without a call-back. D E L .
L O G G E R
M E M O R Y
E X I T
S2.4
The action ends with a long beep.
S T A R T
Delete QC Memory Attention! With [START] the memory assigned to QC data is deleted without a call-back. D E L .
E X I T
RIELE BERLIN
Q C
M E M O R Y
The action ends with a long beep.
S T A R T
Photometer 5010 V5+
3.3.5
11.04.2007
3.3
SERVICE TOOLS
S3
Display Test The display test ist invoked with [START]. For three seconds all pixels of the touch screen are illuminated.
D I S P L A Y T E S T
The test terminates by itself. E X I T
S4
S T A R T
Lamp The function shows the current lamp voltage. The value is shown in the second line (e.g. 12.13 V). L A M P
With [MIN.] the lamp voltage can be temporarily reduced. In case of the adjustment of the optical path the light spot is better to be seen.
1 2 . 1 3 V
With [MAX.] the lamp voltage arises to its normal value. E X I T
S5
M I N .
Serial No.
SERIAL
NO.
The current serial number of the device can be read and set. The number (e.g. 7001) is shown on the right upper corner of the window.
7001
A new number can be entered with maximum five digits. 1
2
3
4
5
6
ESC
9
0
.
+
-
RIELE BERLIN
7
8
The input has to be confirmed with [E].
E
Photometer 5010 V5+
3.3.6
11.04.2007
3.3 S6
SERVICE TOOLS PCB Layout
P C B
L A Y O U T
The attribut of the PCB layout can be read and set. The attribut (e.g. c)is shown on the right upper corner of the window. Software applications may be depend on this attribut. The attribut is labeled on the PCB RR_120 near plug ST13.
c
The attribut can be changed with [SELECT]. E X I T
S7
O K
S E L E C T
The input has to be confirmed with [OK].
Set Wavelength The assignment of wavelength and filter position can be done. S 1 2 3 4 5
E T W A V E L E N G T H : 3 4 0 6 : 6 2 3 : 4 0 5 7 : 9 9 9 : 4 9 2 8 : 9 9 9 : 5 4 6 9 : 9 9 9 : 5 7 8
E X I T
N E W
The current filter position is blinking. With [SELECT] the filter position can be set. With [NEW] the wavelength can be edited.
S E L E C T
In the first line the current filter position is shown. NEW
WAVELENGTH
The new value of the wavelength has to be entered.
1
The input has to be confirmed with [E]. 1
2
3
4
5
6
ESC
9
0
.
+
-
RIELE BERLIN
7
8 E
Photometer 5010 V5+
3.3.7
11.04.2007
3.3 S8
SERVICE TOOLS Test Filter Wheel The rotation of the filter wheel can be controlled. TEST F1 F1
S9
FILTER 340nm
F2
F3
ESCF9
F0
F4
With [F0] up to [F9] the discret positions of the filter wheel can be selected.
WHEEL 399MS
F5
F6
F7
F8
A specific value (e.g. 399MS) shows the rotation of the filter wheel. The abbreviation MS is used for motor step.
Temperature ON/OFF
TEMPERATURE O 2 3 3
F 5 0 7
F C C C
EXIT
R A S C
E C T O
OK
Q T A N
U U B V
37C I A I E
ON
RED L LITY RTER
D
14165 14053 4 947
SELECT
The temperature regulation can be switched on or switched off. The current setting is shown in the first line as ON or OFF. The display shows aditionally the following information: • • • • • •
a D in case of an inserted flow through cuvette adaptor an E in case of an inserted standard cuvette adaptor the required value of temperature regulation the actual value of temperature regulation the stability value the current value of ADC
With [SELECT] the temperature level can be choosen. With [OK] the temperature regulation is invoked.
RIELE BERLIN
Photometer 5010 V5+
3.3.8
11.04.2007
3.3 S10
SERVICE TOOLS Temperature Value The temperature values of the device can be read and set. A new value can be entered with maximum five digits.
TEMPERATURE VALUE E25 8322 D25 8299 E30
9552
D30
9485
E37
11273
D37
11165
EXIT
NEW
SELECT
With [SELECT] a specific temperature value can be set. The temperature values are marked as E25, E30, E37, D25, D30 and D37 corressponding to the type of adaptor (E or D) and the temperature level (25° C, 30° C and 37° C). The current temperature value is blinking. With [NEW] a temperature value can be edited. Enter temperature value. Confirm with [E].
NEW
S11
(chapter 4.40 - Thermal adjustment)
VALUE
1
2
3
4
5
6
ESC
9
0
.
+
-
7
8 E
Correction Value
CORRECTION E25 3000
VALUE D25 3000
E30
3000
D30
3000
E37
3000
D37
3000
EXIT
NEW
The correction values of the device can be read and set. A new value can be entered with maximum five digits.
SELECT
With [SELECT] a specific correction value can be set. The correction values are marked as E25, E30, E37, D25, D30 and D37 corresponding to the type of adapter (E or D) and the temperature level (25° C, 30° C and 37° C). The current correction value is blinking. With [NEW] a correction value can be edited. Enter correction value. Confirm with [E].
NEW
VALUE
1
2
3
4
5
6
ESC
9
0
.
+
-
RIELE BERLIN
7
8 E
Photometer 5010 V5+
3.3.9
11.04.2007
3.3 S12
SERVICE TOOLS Temperature Calibration
TEMP.
The correction of the temperature regulation is possible by input of the temperature value which is measured in the cuvette with an external measuring system (Thermistor and DMM).
CALIBRATING
TEMP.
ACTUAL
:
1
2
3
4
5
6
ESC
9
0
.
+
-
7
This function is available only when the temperature regulation is stabilized. In an unstable condition an error message is shown on the display.
8
Enter the actual temperature value always four-digit in ° C e.g. [3] [7] [.] [2] [0] measured by an external measuring system.
E
Confirm with [E]. The system calculates and stores the new setting for the selected temperature level and starts working with it at once.
S13
ADC Correction The current ADC correction of the device can be read and set. ADC
S14
CORRECTION
150
1
2
3
4
5
6
ESC
9
0
.
+
-
7
The range of this value is 0 up to 255. The default value is 150. The input has to be confirmed with [E].
8 E
ADC Counts (Optic) Indicated is the current value of the optical analoguedigital converter. The value is proportional to the lightcurrent depending on the selected amplification level and the boost setting.
ADC COUNTS (OPTIC) BOOST OFF V0 F1 340nm 18810 V0
V1
V2
V3
V4
V5
V6
V7
F0
F1
F2
F3
F4
F5
F6
F7
B0. F8
F9
ESC
To a key actuation the system reacts possibly only after three seconds. The functions V0 to V7 set the amplification level. The function BO increases (boost ON) or reduces (boost OFF) the time slot. The functions F0 to F9 place the filter wheel into the positions 0 to 9. The position 0 corresponds to the filter wheel position at the dark level adjustment. Stop function by [ESC].
RIELE BERLIN
Photometer 5010 V5+
3.3.10
11.04.2007
3.3
SERVICE TOOLS
S15
ADC Counts (Dark Adjustment) The ADC values of the dark adjustment can be read. ADC
COUNTS
0 2 4 6
1 1 1 1
: : : :
8 8 8 8
4 4 4 5
2 3 5 3
5 3 7 4
(DA E 1 3 5 7
RK
ADJ.)
: : : :
1 1 1 1
EXIT
8 8 8 8
4 4 4 6
2 4 8 3
8 2 3 0
The display shows the following information: • • •
E/D
a D in case of a preselected flow through cuvette adaptor an E in case of a preselected standard cuvette adaptor the ADC values assigned to the amplification levels V0 up to V7
With [E/D] the display shows alternately the values in combination with the different adaptors E or D.
S16
Boost Wavelength
BOOST
WAVELENG
To improve the low light gain at small wavelengths, the measurement time is extended when operating below a definite wavelength. This definite wavelength will be displayed on the right upper corner of the window.
390nm
1
2
3
4
5
6
ESC
9
0
.
+
-
7
8
The default value is 390 nm. The valid range is limited by 340 nm and 900 nm. The input has to be confirmed with [E].
RIELE BERLIN
E
Photometer 5010 V5+
3.3.11
11.04.2007
3.3 S17
SERVICE TOOLS Calibration 12V ADC In the first line the actual value of the voltage 12 VDC is to be seen. C A L . 1 2 V
A D C
1 2 . 0 2 V
The internal measuring unit can be calibrated by invoking [START]. It is assumed that the adjustment of the voltage 12 VDC of halogen lamp is done properly (chapter 4.40.4).
E X I T
S18
S T A R T
The procedure is finished by [EXIT].
Distributor Text Init
D I S T R I B U T O R
T E X T
I N I T
The distributor text shown after switching on the Photometer 5010 V5+ will be erased by invoking [START]. A new distributor text can be set by software “Serial Monitor 5010” (chapter 4.9).
E X I T
RIELE BERLIN
S T A R T
Photometer 5010 V5+
3.3.12
11.04.2007
3.3
SERVICE TOOLS
S19
Automatic Results This operation works only with standard cuvette adapter! A U T O M A T I C
R E S U L T S
E X I T
METHOD 1 2 3 4 5 6 7 8
1
W F T D U
A A E E N
V C M L I
2
E T P A T
C/F
4
340nm 1.000 37C 0s
5
6
5
7
EXIT OK
8
P1/3
For each setting parameter a leading identification number is shown. If the identification number is selected on the keyboard, the corresponding setting parameter becomes configurable. After setting parameters like wavelength, factor, delay time, unit, … the procedure is started by [OK]. By setting delay time the intervals between the measurements can be defined.
The procedure is working in an open loop and produces results as long as the [EXIT] is pushed.
C/F
1.234
By [ZERO] an automatic zero adjustment can be done. To a key actuation the system reacts possibly only after three seconds.
EXIT
S20
1
LENGTH OR ERATURE Y
DELAY
42
S T A R T
NO.
3
The function invokes by [START] a test based on the calculation procedure 1 ↔ C/F (OM chapter 5.4.1).
MODE
WASH
ZERO
RESULT
BCC Of Method/Curve
B C C
O F
M E T H O D / C U R V E
To check the correct storing of a user-defined method or curve, the special test byte BCC concerning the data in the memory is used. With this service program the BCC of a method can be determined. With [M] the BCC of a method can be tested. With [C] the BCC of a curve can be tested.
E X I T
RIELE BERLIN
M
C
Photometer 5010 V5+
3.3.13
11.04.2007
3.4
PLUG IDENTIFICATION
In the device all plug-in connections are marked with P1 ... P23 as described below: PLUG
FROM
⇒
TO
•
P1
AC / DC power supply
⇒
motherboard pcb RR-120
•
P2
motherboard pcb RR-120
⇒
sip control peristaltic pump
•
P3
motherboard pcb RR-120
⇒
fan
•
P4
motherboard pcb RR-120
⇒
halogen lamp
•
P5
motherboard pcb RR-120
⇒
light barrier filter wheel
•
P7
motherboard pcb RR-120
⇒
photodiode
•
P8
motherboard pcb RR-120
⇒
Peltier element
•
P9
motherboard pcb RR-120
⇒
Pcb RR-122 for display, printer, touch
•
P10
motherboard pcb RR-120
⇒
Pcb RR-122 for display, printer, touch
•
P11
motherboard pcb RR-120
⇒
Pcb RR-121 motor filter wheel
•
P13
motherboard pcb RR-120
⇒
Interface RS-232
•
P14
motherboard pcb RR-120
⇒
Pcb RR-121 motor peristaltic pump
•
P20
motherboard pcb RR-120
⇒
Pcb RR-126 cuvette adaptor
•
P21
motherboard pcb RR-120
⇒
Pcb RR-126 cuvette adaptor
•
P23
motherboard pcb RR-120
⇒
Pcb printer controller CN2
RIELE BERLIN
Photometer 5010 V5+
3.4.1
15.05.2006
4.1
MAINS POWER CIRCUIT
Function Description The mains power circuit shows the design of the complete primary circuit. Components of the circuit are: •
Mains connector
•
Mains fuses Fa and Fb
•
Mains switch
•
AC/DC power supply
•
Line filter
•
Ground connection
•
Wires
REF 5010-018
REF 5010-016
Location Backside of Photometer 5010 V5+, base plate Replacement • see chapter 5.1 • see chapter 5.2 • see chapter 5.3 • see chapter 5.6
Opening Separating top case and bottom case Base plate Power supply
Installation For installation proceed in reverse order. Fuses Between the mains switch and the mains cable connector there is a cartridge with two fuses Fa and Fb with following specifications: •
dimensions [mm] :
5 * 20
•
standard:
IEC 60127-2/V
•
time-current characteristic: time lag (T)
•
voltage rating:
250 V
•
rated current:
1.6 A
•
marking:
T 1.6 A H
RIELE BERLIN
Photometer 5010 V5+
4.1.1
13.11.2006
4.1
MAINS POWER CIRCUIT
gnye
power input 90 V~ - 264 V~
gnye
fuses Fa and Fb
ground connection wh
gr
mains switch
br
bk
line filter
br
PE
bk
N
L AC / DC power supply
RIELE BERLIN
Photometer 5010 V5+
4.1.2
13.11.2006
4.2
MAINS CONNECTOR
Function Description The unit connects the power input with the AC/DC power supply.
Location Backside of Photometer 5010 V5+, base plate
fan
mains switch
fuses Fa and Fb
power input 90 V~ - 264 V~
RIELE BERLIN
Photometer 5010 V5+
4.2.1
13.11.2006
4.3
AC / DC POWER SUPPLY
REF 5010-016
Function Description The AC / DC power supply is designed for producing following voltages: •
V1: + 5 VDC / 7 A
•
V2: + 15 VDC / 3 A
•
V3: - 15 VDC / 1 A
Location Base plate of Photometer 5010 V5+ Replacement • see chapter 5.1 • see chapter 5.2 • see chapter 5.6
Opening Separating top case and bottom case Power supply
Installation For installation proceed in reverse order. See also exploded view, chapter „Dismantling“
Terminal block
RIELE BERLIN
Photometer 5010 V5+
4.3.1
03.05.2006
4.3
AC / DC POWER SUPPLY
REF 5010-016
P1
Cable AC / DC power supply Capacitor 10000 µF/25 V
RIELE BERLIN
Photometer 5010 V5+
4.3.2
03.05.2006
4.10
OPTICAL UNIT
Function Description Optical unit
The optical unit consists of the following components: •
Filter wheel with filters
•
Step motor filter wheel
•
Light barrier filter wheel
•
Step motor pump
•
Pump wheel
•
Connector bubble detector
•
Halogen lamp
•
Unit photodiode
•
Lenses
•
Unit Peltier element
•
Unit cuvette adaptor
Location Base plate Replacement The Photometer 5010 V5+ has to be dismantled: • • •
see chapter 5.1 Opening see chapter 5.2 Separating top case and bottom case see chapter 5.3 Base plate
Installation For installation proceed in reverse order.
RIELE BERLIN
Photometer 5010 V5+
4.10.1
11.05.2006
4.11
FILTER WHEEL
Function Description The filter wheel has nine filter positions called F1 to F9. Six filter positions are equipped with optical filters of standard wavelengths. The wavelengths are: 340 nm, 405 nm, 492 nm, 546 nm, 578 nm and 623 nm Location Optical unit Replacement The Photometer 5010 V5+ has to be dismantled: • • • •
see chapter 5.1 see chapter 5.2 see chapter 5.3 see chapter 5.5
Opening Separating top case and bottom case Base plate Optical unit: Filter wheel, motor filter wheel, motor peristaltic pump, light barrier
Installation For installation proceed in reverse order.
Filter wheel with nine filter positions called F1 to F9
Motor mounting plate
Filter wheel equipped with interference line filters of standard wavelengths
RIELE BERLIN
Photometer 5010 V5+
4.11.1
05.05.2006
4.11
FILTER WHEEL
Installation of additional optical filters / Replacement of optical filters The filter wheel can be equipped with additional optical filters in the positions 7 to 9. In the standard equipment the not used positions are optically locked by metal slices. The respective metal slice is replaced with a filter at installation of an additional filter. In the memory of the Photometer 5010 V5+ the value of the wavelength (e.g. 670 nm) for the corresponding position (e.g. 7) becomes changed by a service software routine. Mechanical operations: Location Optical unit Replacement The Photometer 5010 V5+ has to be dismantled: • • •
see chapter 5.1 see chapter 5.2 see chapter 5.3
Opening Separating top case and bottom case Base plate – page 5.3.1
The filter wheel is turned by hand so that the corresponding hole is at the cut-out of the motor mounting plate (see page 4.11.1).
Tool B With the tool kit filter exchange ( RIELE REF 5010-001 ) a filter exchange can be easily carried out. The tool kit filter exchange consists of the tools A and B.
Tool A
RIELE BERLIN
Photometer 5010 V5+
4.11.2
05.05.2006
4.11
FILTER WHEEL
With the tool A and a pipe wrench the metal slice can be pushed from the hole in the filter wheel. The cheeks of the pipe wrench grasp at the tool and at the filter wheel. With a screwdriver the Seeger ring and the metal slice can be taken out.
Left picture: Optical filter with marking 340 nm
Right picture: Optical filter with marking arrow
Put the filter into the corresponding hole of the filter wheel. If the filter has only one reflecting surface, this reflecting surface must be judged to the light source. If the filter is marked with an arrow it shows the direction of the light. The Seeger ring can loosely be inserted into the hole in the filter wheel. With the tool B (left illustration) and a pipe wrench the filter and the Seeger ring can be fixed.
RIELE BERLIN
Photometer 5010 V5+
4.11.3
05.05.2006
4.11 1.
FILTER WHEEL
Run UTILITIES – SERVICE TOOLS - SET WAVELENGTH (see chapter 3.3). The assignment of wavelength and filter position can be done. S 1 2 3 4 5
E T W A V E L E N G T H : 3 4 0 6 : 6 2 3 : 4 0 5 7 : 9 9 9 : 4 9 2 8 : 9 9 9 : 5 4 6 9 : 9 9 9 : 5 7 8
E X I T
N E W
The current filter position is blinking. With [SELECT] the filter position can be set. With [NEW] the wavelength can be edited.
S E L E C T
In the first line the current filter position is shown. NEW
2.
WAVELENGTH
1
2
3
4
5
6
ESC
9
0
.
+
-
1
The new value of the wavelength has to be entered. 7
8
The input has to be confirmed with [E].
E
Run UTILITIES – SERVICE TOOLS - ADC COUNTS (OPTIC). SERVICE
TOOLS
PAGE
4/5
ADC COUNTS (OPTIC) BOOST OFF V0 F1 340 nm 21336 V0
V1
V2
V3
V4
V5
V6
V7
F0
F1
F2
F3
F4
F5
F6
F7
BO. F8
ESC
F9
Indicated is the current value of the optical analogue-digital converter. The value is proportional to the light-current depending on the selected amplification level and the boost setting. To a key actuation the system reacts possibly only after three seconds. The functions V0 to V7 set the amplification level. The function BO increases (boost ON) or reduces (boost OFF) the time slot. The functions F0 to F9 place the filter wheel into the positions 0 to 9. The position 0 corresponds to the filter wheel position at the dark level adjustment. Select amplification level V0 and boost OFF. Stop function by [ESC].
3.
The ADC counts of the new wavelength should be within a range of 20.000 and 600.000.
4.
Finish the operation with [ESC].
RIELE BERLIN
Photometer 5010 V5+
4.11.4
05.05.2006
4.12
HALOGEN LAMP
REF 0554871001
Function Description The halogen lamp is working with 12 VDC and an electrical power of 20 W.
Location Optical unit
The Photometer 5010 V5+ has to be dismantled (see chapter 5.1 – Opening – item 3 and item 5).
Unscrew the cylinder head screw.
Remove the lamp shield.
Replacement Unscrew the knurled head screw.
Remove the holding plate.
RIELE BERLIN
Photometer 5010 V5+
4.12.1
05.05.2006
4.12
HALOGEN LAMP
REF 0554871001 The halogen lamp can be easily removed.
Attention: The halogen lamp may be hot. Use tweezers for removing.
The picture shows the lamp socket.
RIELE BERLIN
Photometer 5010 V5+
4.12.2
05.05.2006
4.12
HALOGEN LAMP
REF 0554871001 The plastic bag of the halogen lamp can be used for inserting of the lamp. Do not touch the glass of the halogen lamp with fingers.
The picture shows the plastic bag, the halogen lamp, the holding plate and the lamp shield.
RIELE BERLIN
Photometer 5010 V5+
4.12.3
05.05.2006
4.12
HALOGEN LAMP
REF 0554871001
Installation 1.
For installation proceed in reverse order.
2.
In case of working with the flow-through cuvette adaptor the cell must be filled with distilled water. In case of working with the standard cuvette adaptor the cuvette compartment must be empty.
3.
Switch on the Photometer 5010 V5+.
4.
Check the voltage 12 VDC of halogen lamp (see chapter 4.40.4).
5.
Run UTILITIES – ADC COUNTS (OPTIC). UTILITIES
PAGE
5/5
ADC COUNTS (OPTIC) BOOST OFF V0 F1 340 nm 21336 V0
V1
V2
V3
V4
V5
V6
V7
F0
F1
F2
F3
F4
F5
F6
F7
BO. F8
ESC
F9
Indicated is the current value of the optical analogue-digital converter. The value is proportional to the light-current depending on the selected amplification level and the boost setting. To a key actuation the system reacts possibly only after three seconds. The functions V0 to V7 set the amplification level. The function BO increases (boost ON) or reduces (boost OFF) the time slot. The functions F0 to F9 place the filter wheel into the positions 0 to 9. The position 0 corresponds to the filter wheel position at the dark level adjustment. Select amplification level V0 and boost OFF. Stop function by [ESC].
6.
Check one after another the used positions of the filter wheel.
7.
The ADC counts should be within a range of 20.000 and 600.000 (see chapter 4.40.6 – Adjustment of amplifier stage).
8.
Finish the operation with [ESC].
RIELE BERLIN
Photometer 5010 V5+
4.12.4
05.05.2006
4.13
UNIT PHOTODIODE
REF 5010-056
Function Description The unit photodiode contains a silicon photodiode, a lense, a lense holder and a cable with plug.
Lense
Plug P7
The photodiode is covered by a black label.
Location The photodiode is situated at the optical unit. The plug P7 is connected with the socket ST7 on the mother board RR-120.
Unit photodiode
Plug P7
RIELE BERLIN
Photometer 5010 v5+
4.13.1
10.05.2006
4.13
UNIT PHOTODIODE
REF 5010-056
Replacement The Photometer 5010 v5+ has to be dismantled: •
see chapter 5.1
Opening – item 1 and item 3
Unplug the unit photodiode from the mother board RR-120. Unscrew the two screws attaching the unit photodiode at the optical unit. Installation For installation proceed in reverse order.
RIELE BERLIN
Photometer 5010 v5+
4.13.2
10.05.2006
4.14
UNIT CUVETTE ADAPTOR
REF 5010-068
Function Description A power Darlington transistor TIP 132 is used as a heating unit. The unit contains a transistor TIP 132, the PCB RR-126 cuvette adaptor, cables and two plugs P20 and P21. The plug P21 is connected with the socket ST21 on the mother board RR-120.
The plug P20 is connected with the socket ST20 on the mother board RR-120. Lead with crimped connector belonging to plug P2 pin 4 (type of adaptor)
Location Optical unit Replacement The Photometer 5010 V5+ has to be dismantled: • • •
see chapter 5.1 see chapter 5.2 see chapter 5.3
Opening Separating top case and bottom case Base plate
Unscrew the two cylinder head screws of the PCB RR-126.
Unscrew the cylinder head screw of the transistor.
Installation For installation proceed in reverse order.
RIELE BERLIN
Photometer 5010 V5+
4.14.1
10.05.2006
4.15
UNIT PELTIER ELEMENT
REF 5010-062
Function Description An Peltier element is an electrical element based on the Peltier effect designated after Jean Peltier, which produces a temperature difference between two plates with current flow. The current pumps warmth from a side on the other one and produces a temperature difference between the plates. The element is used to cool down the cuvette, cuvette adaptor and cuvette compartment. Location Optical unit
Unit Peltier element
Replacement The Photometer 5010 V5+ has to be dismantled: • • • •
see chapter 5.1 see chapter 5.2 see chapter 5.3 see chapter 5.4
Opening Separating top case and bottom case Base plate Optical compartment, peristaltic pump, heating unit
Two clamp screws are glued.
Heat conducting paste
Heat sink
Mica washer
Installation For installation proceed in reverse order.
RIELE BERLIN
Photometer 5010 V5+
4.15.1
27.04.2006
4.16
STEP MOTOR PUMP
REF 5010-060
Function Description The peristaltic pump is used for sample aspiration into the flow-through cell. A step motor drives the rotor of the pump.
Location Optical unit Replacement The Photometer 5010 V5+ has to be dismantled: • • • •
see chapter 5.1 see chapter 5.2 see chapter 5.3 see chapter 5.5
Opening Separating top case and bottom case Base plate Optical unit: Filter wheel, motor filter wheel, motor peristaltic pump, light barrier
Installation For installation proceed in reverse order.
RIELE BERLIN
Photometer 5010 V5+
4.16.1
10.05.2006
4.18
STEP MOTOR FILTER WHEEL
REF 5010-061
Function Description The step motor is used for driving the filter wheel.
Location Optical unit Replacement The Photometer 5010 V5+ has to be dismantled: • • • •
see chapter 5.1 see chapter 5.2 see chapter 5.3 see chapter 5.5
Opening Separating top case and bottom case Base plate Optical unit: Filter wheel, motor filter wheel, motor peristaltic pump, light barrier
Installation For installation proceed in reverse order.
RIELE BERLIN
Photometer 5010 V5+
4.18.1
10.05.2006
4.19
LIGHT BARRIER FILTER WHEEL
REF 5010-063
Function Description The light barrier is the position control of the filter wheel. The unit is delivered with a plug.
The plug P5 is connected with the socket ST5 on the mother board RR-120. Holder of the light barrier
Light barrier
Location Optical unit. Replacement The Photometer 5010 V5+ has to be dismantled: • •
see chapter 5.1 see chapter 5.5
Opening – item 1, item 2, item 3, item 5 Optical unit: Filter wheel, motor filter wheel, motor peristaltic pump, light barrier Adjusting procedure of the light barrier: Loosen the cylinder head screw.
Hole on the mounting plate
RIELE BERLIN
Photometer 5010 V5+
4.19.1
11.05.2006
4.19
LIGHT BARRIER FILTER WHEEL
REF 5010-063
Installation For installation proceed in reverse order. 1.
Switch on the Photometer 5010 V5+.
2.
Run UTILITIES – SERVICE TOOLS - TEST FILTER WHEEL. The rotation of the filter wheel can be controlled. TEST F1 F1
FILTER 340nm
F2
F3
ESCF9
F0
F4
With [F0] up to [F9] the discret positions of the filter wheel can be selected.
WHEEL 399MS
F5
F6
F7
F8
A specific value (e.g. 399MS) shows the rotation of the filter wheel. The abbreviation MS is used for motor step. Move the holder up or down as long as each hole situated on the border line of the filter wheel is centered to the hole situated on the mounting plate. Fix the cylinder head screw.
3.
Check one after another the used positions of the filter wheel.
4.
Finish the operation with [ESC].
RIELE BERLIN
Photometer 5010 V5+
4.19.2
11.05.2006
4.20
BUBBLE DETECTOR WITH PLUG
REF 1706870001
Function Description The bubble detector controls the aspirated volume by an integrated light barrier. The detector gives an analogue signal showing the status AIR or WATER. The signal is controlled by the microcontroller and produces in case of a wrong handling or technical defective an error message. For further information see OPERATING INSTRUCTIONS, chapter 9 – Error message / Correction.
Location
Bubble detector
Replacement Open the lid. Disconnect the bubble detector. Withdraw the aspiration tubing from the metal tube. Don’t bend the apiration tubing. Replace the bubble detector. Installation For installation proceed in reverse order. For further information see OPERATING INSTRUCTIONS, chapter 7.2.4 – Pump / bubble detector.
RIELE BERLIN
Photometer 5010 V5+
4.20.1
11.05.2006
4.21
FLOW-THROUGH CUVETTE ADAPTOR
REF 1704796001
Function Description The flow-through cuvette adaptor consists of: • • • • •
Plastic adaptor Flow-through cuvette with a volume of 32 µl Heating unit Plug Potentiometer for factory calibration
Location Optical unit Replacement Loosen clamp screw, open tube connection, remove adaptor. Installation For installation proceed in reverse order. After installation of a new cuvette adaptor a dark level adjustment has to be done by [UTILITIES] [DARK LEVEL ADJUSTMENT] [START].
RIELE BERLIN
Photometer 5010 V5+
4.21.1
28.04.2006
4.22
STANDARD CUVETTE ADAPTOR
REF 1704800001
Function Description The standard cuvette adaptor is used for disposable cuvettes or for working with a discrete flow-through cell. The standard cuvette adaptor consists of: • • • •
Plastic adapter Heating unit Plug Potentiometer for factory calibration
Location Optical unit Replacement Loosen clamp screw, remove adaptor. Installation For installation proceed in reverse order. After installation of a new cuvette adaptor a dark level adjustment has to be done by [UTILITIES] [DARK LEVEL ADJUSTMENT] [START]. Holding spring for disp. cuvette REF 1707159001
RIELE BERLIN
Photometer 5010 V5+
4.22.1
28.04.2006
4.23
CONNECTOR BUBBLE DETECTOR
REF 5010-069
Function Description The connector bubble detector connects the bubble detector with PCB RR-120 motherboard.
The plug P2 is connected with the socket ST2 on the mother board RR-120.
Location Optical unit
Connector bubble detector
Replacement The Photometer 5010 V5+ has to be dismantled: •
see chapter 5.1
Opening – item 1, item 2, item 3, item 5
Loosen milled nut and remove the connector bubble detector. Remove the green lead with crimped connector belonging to plug P2 pin 4 (type of adaptor). Installation For installation proceed in reverse order.
RIELE BERLIN
Photometer 5010 V5+
4.23.1
11.05.2006
4.24
LAMP HOLDER
REF 5010-059
Function Description The lamp holder is used for fixing the halogen lamp.
Ondular washer
The plug P4 is connected with the socket ST4 on the mother board RR-120.
Location Optical unit
Lamp holder
Cylinder head screws with ondular washer
Replacement The Photometer 5010 V5+ has to be dismantled: •
see chapter 5.1
Opening – item 1, item 2, item 3, item 5
Remove halogen lamp. For further information see chapter 4.12 – Halogen lamp. Unplug P4. Unscrew the two cylinder head screws. Installation For installation proceed in reverse order.
RIELE BERLIN
Photometer 5010 V5+
4.24.1
12.05.2006
4.26
TUBES
pump tube with joints REF 5010-050
outlet tube cuvette REF 5010-066
waste tubing REF 1704834001
aspiration tubing REF 5010-065
Joint inlet tube cuvette REF 1707175001
RIELE BERLIN
Aspiration tubing
REF 5010-065
Joint inlet tube cuvette
REF 1707175001
Outlet tube cuvette
REF 5010-066
Pump tube with joints
REF 5010-050
Waste tubing
REF 1704834001
Photometer 5010 V5+
4.26.1
18.05.2006
4.30
TOP CASE
REF 5010-064
Function Description The top case is one of the two main parts of the housing. It is assembled with: •
Microswitch (see chapter 4.33)
Location Top case Replacement The Photometer 5010 V5+ has to be dismantled: • • •
see chapter 5.1 Opening – item 1, item 2 and item 5 see chapter 5.2 Separating the top case from the bottom case see chapter 4.41 Touchscreen
Note: When the plastic foil touchscreen (REF 5010-070) is removed from device it might not be reusable. Installation For installation proceed in reverse order.
RIELE BERLIN
Photometer 5010 V5+
4.30.1
15.05.2006
4.31
TOP CASE PRE-ASSEMBLED
REF 5010-071
Function Description The top case is one of the two main parts of the housing. It is assembled with: • • • •
Sipping lever Microswitch (see chapter 4.33) Metal tube inlet Lid
Location Top case Replacement The Photometer 5010 V5+ has to be dismantled: • • •
see chapter 5.1 Opening – item 1, item 2 and item 5 see chapter 5.2 Separating the top case from the bottom case see chapter 4.41 Touchscreen
Note: When the plastic foil touchscreen (REF 5010-070) is removed from device it might not be reusable. Installation For installation proceed in reverse order.
RIELE BERLIN
Photometer 5010 V5+
4.31.1
15.05.2006
4.32
METAL TUBE INLET
REF 5010-055
Function Description The metal tube inlet is used to guide the aspirate tubing.
Metal tube inlet
Location Top case Replacement Remove aspirate tubing from metal tube inlet. Remove metal tube inlet by turning and pulling at the same time. Installation For installation proceed in reverse order.
RIELE BERLIN
Photometer 5010 V5+
4.32.1
15.05.2006
4.33
MICROSWITCH WITH PLUG
REF 5010-072
Function Description The microswitch is pressed by the sipping lever, its signal starts the peristaltic pump.
Location Top case Replacement The Photometer 5010 V5+ has to be dismantled: • •
see chapter 5.1 see chapter 5.2
Opening Separating the top case from the bottom case
Disconnect plug J/ST3 of PCB RR-122 display, printer, touch. Remove microswitch by a screw driver and clean frame from remaining glue. Installation For installation proceed in reverse order. Use for example glue „Delomet Typ 2301“.
RIELE BERLIN
Photometer 5010 V5+
4.33.1
15.05.2006
4.34
PLASTIC FOIL TOUCHSCREEN
REF 5010-070
Function Description The plastic foil is made of polyester and self adhesive.
Location Top case Replacement Remove the plastic foil carefully. Avoid scratches on the top case. Note: When the plastic foil is removed from device it might not be reusable. Installation For installation proceed in reverse order.
RIELE BERLIN
Photometer 5010 V5+
4.34.1
12.05.2006
4.35
PRINTER MECHANISM 8NAD
REF 5010-052
Function Description The printer is shown with an inserted ribbon.
Printer ribbon REF 1010735001 or REF 0745235001
Location Top case Replacement The Photometer 5010 V5+ has to be dismantled: • •
see chapter 5.1 see chapter 5.2
Opening Separating top case and bottom case
Remove top cover for printer, paper and ribbon. Disconnect 18-pin plug CN6 from printer controller 8NAD (REF 5010-053). Loosen 2 front screws in the hollows and remove the other 2 screws. Remove printer mechanism.
RIELE BERLIN
Photometer 5010 V5+
4.35.1
15.05.2006
4.35
PRINTER MECHANISM 8NAD
REF 5010-052
Installation For installation proceed in reverse order.
Marked lead
Solder printer connection cable to printer mechanism. Also take care for marked lead of pin 1.
RIELE BERLIN
Photometer 5010 V5+
4.35.2
15.05.2006
4.40
PCB RR-120 MOTHERBOARD
REF 5010-120
Function Description The mother board is the central electronic unit within Photometer 5010 V5+. Following components are equipped on the PCB: •
multiprocessor system based on microcontrollers SAFC517 and 87C51/52
•
Flash memory
•
battery powered ram
•
battery powered real time clock
•
ADC for photocurrent
•
ADC for temperature control
•
interface stepper motor for peristaltic pump
•
interface infrared bubble detector
•
interface stepper motor for filter wheel
•
interface light barrier filter wheel
•
interface RS 232C
•
interface internal printer (Centronics)
•
interface touchscreen
•
interface Peltier element
•
interface heating unit
•
halogen lamp regulator
•
fuses F1, F2 and F3
Location Base plate Replacement The Photometer 5010 V5+ has to be dismantled: • • • •
see chapter 5.1 see chapter 5.2 see chapter 5.3 see chapter 5.7
Opening Separating top case and bottom case Base plate Motherboard
Installation For installation proceed in reverse order. Perform the installation instruction of this chapter.
RIELE BERLIN
Photometer 5010 V5+
4.40.1
11.04.2007
4.40
PCB RR-120 MOTHERBOARD
REF 5010-120
Component diagram
RIELE BERLIN
Photometer 5010 V5+
4.40.2
11.04.2007
4.40
PCB RR-120 MOTHERBOARD
REF 5010-120
Voltages 1. Before adjustment switch the Photometer 5010 V5+ off. Open the device. Open the photometer regarding safety rules of chapter 1 of this service manual (chapter 5.1 Opening). 2. The device plug of the mains cable must be put into the mains cable socket at the back of the device and the mains plug of the mains cable must be put into a grounded mains socket. 3. Switch the device on. 4. Use DVM for measuring ± 12 VDC. 5. Connect the negative lead of DVM to the ground connector (soldering connector). 6. Connect the positive lead of DVM to the pin 3 of IC10. 7. Check the voltage + 5 VDC (tolerance ± 5%). 8. Connect the positive lead of DVM to the pin 4 of IC10. 9. Check the voltage - 12 VDC (tolerance ± 5%). 10. Connect the positive lead of DVM to the pin 14 of IC10. 11. Check the voltage + 12 VDC (tolerance ± 5%). 12. Connect the positive lead of DVM to the battery BT1. 13. Check the voltage + 3 VDC (tolerance ± 10%). + 3 VDC ± 10%
+ 5 VDC ± 5% RIELE BERLIN
- 12 VDC ± 5% Photometer 5010 V5+
+ 12 VDC ± 5% 4.40.3
Ground connector 11.04.2007
4.40
PCB RR-120 MOTHERBOARD
REF 5010-120
Voltage 12 VDC of halogen lamp 1. Before adjustment switch the Photometer 5010 V5+ off. Open the device. Open the photometer regarding safety rules of chapter 1 of this service manual (chapter 5.1 Opening). 2. Switch the device on. 3. Use DVM for measuring 12 VDC. 4. Connect the negative lead of DVM to pin 2 of plug ST4. 5. Connect the positive lead of DVM to pin 1 of plug ST4. 6. Check the voltage +12.0 VDC (tolerance ± 50 mVDC). 7. If necessary adjust with potentiometer P2. Turning clockwise increases voltage. Turning counter-clockwise decreases voltage.
Potentiometer P2
Fuses F1, F2, and F3 Name F1 F2 F3
RIELE BERLIN
Protection VCC (+ 5 VDC) + 15 VDC / + 12 VDC Power supply printer + 5 VDC
Photometer 5010 V5+
Specification T 3.15 A / 250 V T 6.3 A / 250 V T 6.3 A / 250 V
4.40.4
11.04.2007
4.40
PCB RR-120 MOTHERBOARD
REF 5010-120
Amplifier for photocurrent and ADC The photocurrent of the photodiode is converted with short circuit application into a voltage signal with the aid of the amplifier IC5 (AD 711). Potentiometer P3 sets the amplification and potentiometer P4 sets the offset voltage of the amplifier stage. A voltage / frequency converter IC9 (AD654) is connected to the output of the amplifier. The microcontroller can set the amplification of the amplifier stage by the multiplexer IC10 (ADG428). The eight different settings of amplification are called V0 up to V7. The setting V0 is equivalent to the lowest amplification. The microcontroller converts the frequency signal of the voltage / frequency converter to a digital result in a range between 0 and ~ 600000. This result is called value of the ADC. The value of the ADC in the case of photocurrent = 0 (darkness) is called offset. The offset is •
dependent on the setting of the amplifier stage,
•
positive and 0,
•
set with potentiometer P4.
The following diagram shows the ADC-counter values versus the photocurrent.
ADC ~ 600.000
offset 0 photo current
RIELE BERLIN
Photometer 5010 V5+
4.40.5
11.04.2007
4.40
PCB RR-120 MOTHERBOARD
REF 5010-120
Adjustment of amplifier stage 1. Before starting adjustments switch the Photometer 5010 V5+ off and unplug from the main socket. Open the photometer regarding safety rules of chapter 1 of this service manual. The Photometer 5010 V5+ has to be dismantled (chapter 5.1 – Opening – item 4 and item 5). 2. Insert standard cuvette adaptor for disposable cuvettes into the device, but do not insert cuvette. 3. Switch the device on. 4. Be sure that the optical unit is adjusted faultless (chapter 4.10, 5.4 and 5.5) and check the supply voltages and the lamp voltage (chapter 4.40.3 and 4.40.4). 5. Run UTILITIES – ADC COUNTS (OPTIC). UTILITIES
PAGE
5/5
ADC COUNTS (OPTIC) BOOST OFF V0 F1 340 nm 21336 V0
V1
V2
V3
V4
V5
V6
V7
F0
F1
F2
F3
F4
F5
F6
F7
BO. F8
Indicated is the current value of the optical analogue-digital converter. The value is proportional to the light-current depending on the selected amplification level and the boost setting. To a key actuation the system reacts possibly only after three seconds.
F9
The functions V0 to V7 set the amplification level. The function BO increases (boost ON) or reduces (boost OFF) the time slot. The functions F0 to F9 place the filter wheel into the positions 0 to 9. The position 0 corresponds to the filter wheel position at the dark level adjustment.
ESC
Select amplification level V0 and boost OFF. Stop function by [ESC]. 6. Amplification: Turn the filter wheel to that position by selecting F1 up to F9, so that the shown ADC value becomes maximum. Adjust the ADC value to a maximum of 500000 (tolerance ± 50000) with the potentiometer P3 on the mother board. In case of not reaching this value, turn the potentiometer P3 clockwise (increasing) to the maximum. Then reduce the amplification with turns counter-clockwise (decreasing) until ADC value is changing. 7. Offset: Switch the device off. 8. Replace the plug ST7 – Photodiode connection with the plug 1 Mohm.
plug 1 Mohm
9. Switch the device on. 10. Run UTILITIES – ADC COUNTS (OPTIC). Turn the filter wheel to the dark position by selecting F0. 11. Note the ADC value e.g. 18015.
RIELE BERLIN
Photometer 5010 V5+
4.40.6
11.04.2007
4.40
PCB RR-120 MOTHERBOARD
REF 5010-120
12. Select amplification level V7. 13. Adjust the ADC value with potentiometer P4 to the noted value e.g. 18015 with a tolerance of ± 50 counts. 14. Check all sensitivity levels V0 up to V7. The ADC values shall increase or decrease step by step. For example valid values for the increasing case could be: V0: 18865, V1: 18869, V2: 18872, V3: 18887, V4: 18899, V5: 18901, V6: 18923, V7: 18933 The deviation between V0 and V7 shall be smaller than absolute 200 counts. 15. Switch the device off. 16. Replace the plug 1 Mohm – Photodiode connection with the plug ST7. 17. Do a dark level adjustment by applying utility U1 with both cuvette adaptors. (OM chapter 7.2.1 - Dark level adjustment).
P3
RIELE BERLIN
MP2
Photometer 5010 V5+
P4
Ground connector
4.40.7
11.04.2007
4.40
PCB RR-120 MOTHERBOARD
REF 5010-120
Temperature control General Description The temperature control takes care of the correct temperature inside the flow-through cuvette or inside the standard cuvette adaptor. The temperature is selectable by the program at fixed levels, i.e. 25 °C, 30 °C or 37 °C. The temperature control circuit consists of: •
microcontroller system
•
temperature sensor
•
analogue-digital-converter (ADC)
•
heating unit
•
cooling unit
Microcontroller System The temperature control circuit is a PID-controller, a software module in the microcontroller system. The PIDcontroller works in the background all the time. Dependent on the cuvette adaptor the PID-controller uses different parameters. The required value of temperature is ascertained by the program of the microprocessor system. The temperature is controlled according to the set temperature in the selected method. The PID-controller gives information to the controlling program about the given temperature tolerances and therefore about the readiness for measuring. Temperature Sensor and ADC The voltage signal of the temperature sensor LM335 is converted by the IC2 (LTC1286) to an equivalent frequency. The microcontroller unit IC3 (87C52PLCC) converts the frequency to a digital value. The voltage signal of the temperature sensor LM335 is typical 3.1 VDC at 37 °C, 3.03 VDC at 30 °C and 2.98 VDC at 25 °C. The ADC converts the input voltage to a digital value between 0 and 32767 as shown on following diagram:
ADC 32767
ca. 17000
ca. 15200
ca. 13900
0 2,98 VDC
3,03 VDC
3,1 VDC
input voltage of temperature sensor
RIELE BERLIN
Photometer 5010 V5+
4.40.8
11.04.2007
4.40
PCB RR-120 MOTHERBOARD
REF 5010-120
Heating The control variable of the controller has influence on the heating. A switched Darlington transistor in source of power-switching is used as heating element. The PID-controller produces a voltage at the emitter of the Darlington transistor by a pulse-width-modulation. This voltage controls the current through the transistor. Involved components are the microcontroller IC3 (87C52PLCC) and IC7A/IC7B (LM393). Cooling For cooling or counter cooling during heating the microcontroller system switches on a Peltier element. There are four power levels with following terms and meaning: 0 = Cooling out 1 = Cooling (low level) 2 = Cooling (middle level) 3 = Cooling (high level) In case of temperature changing by method selection cooling level 0 is selected if desired temperature is higher as selected one. In case of temperature changing by method selection cooling level 3 is selected if desired temperature is lower as selected one. If selected temperature is reached it is switched over to a cooling level depending on the temperature: •
for 37 degrees Centigrade ⇒ cooling level 1
•
for 30 degrees Centigrade ⇒ cooling level 2
•
for 25 degrees Centigrade ⇒ cooling level 3
The cooling element is a Peltier element. The Peltier element functions as a heat pump and transports heat out of the cuvette adaptor to the heat sink. The heat sink is provided with air by the fan of the device and transports the heat outside to the area. The Peltier element operates with a voltage, switchable on four levels: •
cooling level 0 ⇒ 0
•
cooling level 1 ⇒ 0.45 VDC
•
cooling level 2 ⇒ 0.9 VDC
•
cooling level 3 ⇒ 1.3 VDC
VDC
Involved components are the microcontroller unit IC3 (87C52PLCC), the OP-booster U1A (LM358), the transistor T4 (BC547), the transistor T5 (TIP132 mounted at the base plate), the transistor T4 (BC547) and the Peltier element.
RIELE BERLIN
Photometer 5010 V5+
4.40.9
11.04.2007
4.40
PCB RR-120 MOTHERBOARD
REF 5010-120
Thermal adjustment The PID-controller algorithm works always as follows: ACTUAL VALUE
=
REQUIRED VALUE
REQUIRED VALUE
=
TEMPERATURE VALUE
ACTUAL VALUE
⇔
Actual value of the ADC
REQUIRED VALUE
⇔
Sum of TEMPERATURE VALUE + CORRECTION VALUE
TEMPERATURE VALUE
⇔
Value assigned to a temperature level of 37 °C, 30 °C or 25 °C. This value is automatically selected by the device according to the state of operating. The temperature value is board-characteristic and cuvette adaptor specific. The values for the temperature levels of 37 °C, 30 °C or 25 °C are ascertained in factory and noted during the check of the board. They are stored within the battery powered RAM. Dependent on the used cuvette adaptor different service programs can show and edit the values stored in battery powered RAM. The system can be adjusted to lower or higher temperature values. A change of about 25 counts means a temperature change of 0.1 °C.
+
CORRECTION VALUE
Explanation:
RR_120
7000 S
FTH
25 °C
8255
8230
30 °C
9495
9430
37 °C
11198
11113
CORRECTION VALUE
RIELE BERLIN
⇔
•
Service program S10 - Temperature value is used for editing the value (chapter 3.3)
•
Service program S12 - Temperature calibration calibrates the system automatically. (chapter 3.3 / chapter 9.1)
The value is noted on a sticker. The sticker is shown on the left and put on top of the IC15 (SABC517). The sticker shows the serial number of the board (e.g. 7000) and the temperature values of 25 °C, 30 °C, 37 °C of standard cuvette adaptor (S) and flow-through adaptor (FTH). In addition the values are documented in the device card of Photometer 5010 V5+ and stored in a file at the manufacturer.
Correction value in the range of 0 and 9999. The default value is 3000. After using the service program S1 - System initialization (chapter 3.3) the correction value is set to the default value 3000. The system can be adjusted to a lower or a higher temperature value. A change of about 25 counts means a temperature change of 0.1 °C. •
Service program S11 - Correction value is used for editing the value (chapter 3.3)
•
Utility programs U10 - Temperature adjustment adjusts the system automatically. (chapter OM 7.2.10)
Photometer 5010 V5+
4.40.10
11.04.2007
4.40
PCB RR-120 MOTHERBOARD
REF 5010-120
Installation instruction In case of changing the mother board following items have to be done after switching on the device: 1. Check the contrast of LCD (Service Manual chapter 4.42 – Contrast of touchscreen). 2. Check the voltage 12 VDC of halogen lamp. The voltage was pre-adjusted at the factory (Service Manual chapter 4.40 – Voltage 12 VDC of halogen lamp). 3. Check voltages + 12 VDC, - 12 VDC, + 5 VDC and + 3 VDC (battery power) (Service Manual chapter 4.40 – Voltages). 4. Perform the adjustment of amplifier stage (Service Manual chapter 4.40 – Adjustment of amplifier stage). 5. The display of Photometer 5010 V5+ shows: M A I N
M E N U
M E A S U R E
W I T H
P R O G R .
M E A S U R E
W I T H
B A S I C
M E T H O D S
N E W
U T I L I T I E S 3 7 . 0 5 C
/
M E T H O D S M E T H O D S
C H A N G E
/
L A M P 0 4 . 0 1 . 0 6
C O P Y L F 1 1 : 3 9
6. The serial number can be set by applying the service tool S5 (Service Manual chapter 3.3 – S5 - Serial no.). 7. The assignment of filter positions has to be done if additional filters has been built in (e.g. 670 nm). Apply the service tool S7 (Service Manual chapter 3.3 – S7 – Set wavelength). 8. Do a setting of the temperature values by performing the calibration procedure of Photometer 5010 V5+. (Service Manual chapter 9.1 - Subject: Cuvette temperature) (Service Manual chapter 4.40 - Thermal adjustment) 9. Do a setting of “attribute of PCB layout“ by applying the service tool S6: Software applications may be dependent on this attribute (Service Manual chapter 3.3 – S6 - PCB layout). 10. Delete allocated memory by applying the service tool S2: (Service Manual chapter 3.3 – S2.1 – Delete free methods) (Service Manual chapter 3.3 – S2.2 – Multi-standard memory) (Service Manual chapter 3.3 – S2.3 – Delete logger memory) (Service Manual chapter 3.3 – S2.4 – Delete QC memory) 11. Define the laboratory name by applying the utility U11: The name of the laboratory can be stored permanently (OM chapter 7.2.11 – Laboratory name). 12. Define the user names by applying the utility U12: The names of maximum five users can be stored permanently (OM chapter 7.2.12 – User name). 13. Do a dark level adjustment by applying utility U1 in combination with flow-through system. (OM chapter 7.2.1 - Dark level adjustment). The utility is working specifically to the current adaptor. An adjustment of the standard cuvette adaptor should be done later on. 14. Perform a media test by applying utility U4.4 (OM chapter 7.2.4.2 – Media test). RIELE BERLIN
Photometer 5010 V5+
4.40.11
11.04.2007
4.40
PCB RR-120 MOTHERBOARD
REF 5010-120
15. Perform a pump calibration by applying utility U4.2 (OM chapter 7.2.4.4 – Pump calibration). 16. Switch off Photometer 5010 V5+. Change the cuvette adaptor. Switch on Photometer 5010 V5+. 17. Do a dark level adjustment by applying utility U1 in combination with standard cuvette adapter. See OM chapter 7.2.1 - Dark level adjustment. 18. Control the settings by applying utility U7 (OM chapter 7.2.7 - Printing settings).
RIELE BERLIN
Photometer 5010 V5+
4.40.12
11.04.2007
4.40
PCB RR-120 MOTHERBOARD
RIELE BERLIN
Photometer 5010 V5+
REF 5010-120
4.40.13
26.04.2006
4.40
PCB RR-120 MOTHERBOARD
RIELE BERLIN
Photometer 5010 V5+
REF 5010-120
4.40.14
26.04.2006
4.40
PCB RR-120 MOTHERBOARD
RIELE BERLIN
Photometer 5010 V5+
REF 5010-120
4.40.15
26.04.2006
4.40
PCB RR-120 MOTHERBOARD
RIELE BERLIN
Photometer 5010 V5+
REF 5010-120
4.40.16
26.04.2006
4.40
PCB RR-120 MOTHERBOARD
RIELE BERLIN
Photometer 5010 V5+
REF 5010-120
4.40.17
26.04.2006
4.40
PCB RR-120 MOTHERBOARD
REF 5010-120
33 R
RIELE BERLIN
Photometer 5010 V5+
4.40.18
26.04.2006
4.40
PCB RR-120 MOTHERBOARD
REF 5010-120
Attention! The following parts are not equipped on the motherboard. Circuit diagram page 4.40.15 P1
Potentiometer
10K
Circuit diagram page 4.40.18 D1, D2 R25 R24 R11 ST6 T1
Diode Resistor Resistor Resistor Header Transistor
1N4148 1K5 5K6 10K 1x4 BC547
Attention! The following parts of the circuit diagrams are not specified. Circuit diagram page 4.40.15 R13
RIELE BERLIN
Resistor
Photometer 5010 V5+
4R7
4.40.19
26.04.2006
4.41
TOUCHSCREEN
REF 5010-057
Function Description The touchscreen is the control for direct functions and alphanumerical inputs and a graphic display. Location Top case Replacement The Photometer 5010 V5+ has to be dismantled: • •
see chapter 5.1 see chapter 5.2
Opening Separating top case and bottom case
Installation For installation proceed in reverse order. Front view of the touchscreen
J/ST9
J/ST8
J/ST1
Back view of the touchscreen
Touchscreen with PCB RR-122
PCB RR-122
RIELE BERLIN
Photometer 5010 V5+
4.41.1
25.04.2006
4.41
TOUCHSCREEN
REF 5010-057 Touchscreen with PCB RR-122
Touchscreen with PCB RR-122
Flat cable of J/ST8
RIELE BERLIN
Photometer 5010 V5+
4.41.2
25.04.2006
4.41
TOUCHSCREEN
REF 5010-057 Touchscreen with PCB RR-122
Flat cable of J/ST8
Touchscreen with PCB RR-122 inside of the top case
Mounting parts of the touchscreen: With the spacer the position of the touchscreen can be adjusted. The nut has to be unscrewed.
Nut
Spacer
Spring
RIELE BERLIN
Photometer 5010 V5+
4.41.3
25.04.2006
4.42
TOUCH PANEL
REF 5010-067
Function Description The touch panel is an input device employing a material of transparent electrodes formed on glass-film transparent board. Location Top case, placed directly on top of the display device. Replacement The Photometer 5010 V5+ has to be dismantled: • • •
see chapter 5.1 see chapter 5.2 see chapter 4.41
Opening Separating top case and bottom case Touchscreen
Installation For installation proceed in reverse order.
RIELE BERLIN
Photometer 5010 V5+
4.42.1
25.04.2006
4.43
PCB RR-122 DISPLAY, PRINTER, TOUCH
REF 5010-122
Function Description The PCB RR-122 for display, printer and touchscreen connects the following components: •
PCB RR-120 Mother board
•
PCB Touchscreen
•
PCB Printer controller
•
Sipping lever
Location Top case Replacement The Photometer 5010 V5+ has to be dismantled: • •
see chapter 5.1 see chapter 5.2
Opening Separating top case and bottom case
Disconnect all plugs from the PCB. The plugs are marked with J/ST1, J/ST2, J/ST3, J/ST4, J/ST7, J/ST8 and J/ST9. The plug J/ST8 is connecting a flat cable by a slider. The slider has to be opened before removing the flat cable. Remove the two screw nuts attaching the PCB with the touchscreen. Installation For installation proceed in reverse order. Do the following adjustments: •
Remove J/ST9 (Display/Backlight) before switching on the device.
•
Switch on the device.
•
Adjust the voltage 3.5 VDC ± 50 mVDC with potentiometer P1 beetween pin 1 (+) and pin 2 (ground) of plug J/ST9.
•
Switch off the device.
•
Insert the plug to the socket.
•
Switch on the device.
•
Control and adjust the voltage 3.5 VDC ± 50 mVDC with potentiometer P1 beetween pin 1 (+) and pin 2 (ground) of plug J/ST9.
•
Adjust the contrast with potentiometer P2.
RIELE BERLIN
Photometer 5010 V5+
4.43.1
10.04.2007
4.43
PCB RR-122 DISPLAY, PRINTER, TOUCH Voltage 3.5 VDC: potentiometer P1
J/ST9 1 PIN 2
REF 5010-122 Contrast: potentiometer P2
J/ST8 with slider Contrast: potentiometer P2
Voltage 3.5 VDC: potentiometer P1
J/ST8 with slider RIELE BERLIN
Photometer 5010 V5+
4.43.2
10.04.2007
4.43
PCB RR-122 DISPLAY, PRINTER, TOUCH
RIELE BERLIN
Photometer 5010 V5+
4.43.3
REF 5010-122
10.04.2007
4.44
PRINTER CONTROLLER 8NAD
REF 5010-053
Function Description The printer controller pcb is a microprocessor subsystem. The printer mechanism is driven by the printer controller pcb. Location Top case Replacement The Photometer 5010 V5+ has to be dismantled: • •
see chapter 5.1 see chapter 5.2
Opening Separating top case and bottom case
Unplug conectors CN2, CN4 and CN6. Remove the four screws fixing the pcb. Installation All switches of SW1 have to be set to OFF. The solder strap B14 has to be closed The solder strap B16 has to be opened. The solder strap B19 has to be opened The solder strap B20 has to be closed. For installation proceed in reverse order.
solder strap B14 solder strap B20
solder strap B16
solder strap B19
RIELE BERLIN
Photometer 5010 V5+
4.44.1
15.05.2006
4.45
PCB RR-121 MOTOR DRIVER
REF 5010-121
Function Description The PCB RR-121 is the interface for the step motor of the filter wheel. Location Base plate Replacement The Photometer 5010 V5+ has to be dismantled: • • •
see chapter 5.1 see chapter 5.2 see chapter 5.3
Opening Separating top case and bottom case Base plate
Disconnect all plugs from the PCB. The plugs are marked with ST1 and ST2. Remove the two screws attaching the PCB with the base plate (chapter 5.6). Installation For installation proceed in reverse order.
ST1
ST2
RIELE BERLIN
Photometer 5010 V5+
4.45.1
25.04.2006
4.45
PCB RR-121 MOTOR DRIVER
RIELE BERLIN
Photometer 5010 V5+
REF 5010-121
4.45.2
25.04.2006
4.50
FAN
REF 1706985001
Function Description The fan has a nominal voltage of 12 VDC. The fans blows the air out of the system for inside cooling. Location Backside of Photometer 5010 V5+, base plate. The plug P3 ist connected with the plug ST3 on the mother board pcb RR-120. Replacement The Photometer 5010 V5+ has to be dismantled: • •
see chapter 5.1 see chapter 5.2
Opening Separating the top case from the bottom case
Unplug P1 and P3 from the PCB RR-120 mother board. Remove the four screws (A). Clean air vents. Installation For installation proceed in reverse order.
A
bl
rd
2 1
RIELE BERLIN
Photometer 5010 V5+
4.50.1
P3
15.05.2006
4.51
BOTTOM CASE
REF 5010-054
Function Description The bottom case is one of the two main parts of the housing. Location Bottom of Photometer 5010 V5+ Replacement The Photometer 5010 V5+ has to be dismantled: • •
see chapter 5.1 see chapter 5.2
Opening Separating the top case from the bottom case
After removing the six lens head screws, the base plate can be separated from the bottom case. The bottom case can then be easily lifted. Installation For installation proceed in reverse order.
RIELE BERLIN
Photometer 5010 V5+
4.51.1
15.05.2006
4.52
CARTON PHOTOMETER 5010
Paper
Paper
1 1 1 2 1 2 1 1 1 1 1
REF 1706896001
Aspirate tubing Axis printer paper Dust cover Fuses for line power Mains cable Printer paper Pump tube with joints Ribbon band printer Standard cuvette adaptor Top cover small for printer Waste tubing
REF 1706853001 REF 1704818001 REF 0552402001 REF 5010-050 REF 1010735001 REF 1704800001 REF 5010-073
Plastic foil
RIELE BERLIN
Photometer 5010 V5+
4.52.1
16.05.2006
5.1
ASSEMBLING / DISMANTLING
Opening:
1. Before opening the machine turn off the main power supply. Remove the the mains cable from the machine.
2. Turn the machine upside down. Lay onto a soft base for protecting the cover.
3. Remove the four lens head screws (A1) to open the machine. A1 4. In case of e.g. dismantling the optical unit or replacing an interference line filter or replacing the power supply: Remove the six lens head screws (B1). A1
B1
5. Take off top case and lay it to the right hand side.
RIELE BERLIN
Photometer 5010 V5+
5.1.1
04.05.2006
5.2
ASSEMBLING / DISMANTLING
Separating the top case from the bottom case
The three connections must be unplugged. 1. Flat cable PCB mother board P10 / PCB RR_122 P4 2. Flat cable PCB mother board P9 / PCB RR_122 P2 3. Cable PCB mother board P23 / PCB printer controller CN2
3. 1. 2.
RIELE BERLIN
Photometer 5010 V5+
5.2.1
04.05.2006
5.3
ASSEMBLING / DISMANTLING
Base plate After removing the six lens head screws, the base plate can be separated from the bottom case. The bottom case can then be easily lifted.
main switch
fan
motherboard
ground connection optical unit
RIELE BERLIN
Photometer 5010 V5+
5.3.1
04.05.2006
5.3
ASSEMBLING / DISMANTLING 1.
Unscrew the lens head screw (A3).
2.
Unscrew the lens head screw (B3).
3.
Remove the ground cable.(C3)
4.
Remove the tube from the joint.
A3
C3
B3
Joint
Tube
RIELE BERLIN
Photometer 5010 V5+
5.3.2
04.05.2006
5.3
ASSEMBLING / DISMANTLING
RIELE BERLIN
Photometer 5010 V5+
5.
The optical unit is seperated from the base plate, but connected by the plugs to the motherboard.
6.
Unplug the connectors: ST4, ST2, ST7, ST20, ST21, ST1, ST14, ST11, ST8, ST6, ST5 and ST13.
7.
Optical unit
5.3.3
04.05.2006
5.4
ASSEMBLING / DISMANTLING
Optical unit: Optical compartment, peristaltic pump, heating unit Optical unit
Remove the lamp shield.
Unscrew the two screws.
RIELE BERLIN
Photometer 5010 V5+
5.4.1
04.05.2006
5.4
ASSEMBLING / DISMANTLING The optical unit is divided in two parts. Motor peristaltic pump
Motor filter wheel
Light barrier filter wheel
Unscrew the four crosshead screws.
Unscrew the two screws.
Drain channel
Connector bubble detector Cover plate
RIELE BERLIN
Photometer 5010 V5+
5.4.2
04.05.2006
5.4
ASSEMBLING / DISMANTLING
Unscrew the two allen screws.
Unscrew the two countersunk screws below the allen screws.
The lens holder is dismounted.
Countersunk screw
RIELE BERLIN
Photometer 5010 V5+
5.4.3
04.05.2006
5.4
ASSEMBLING / DISMANTLING The unit photodiode is dismounted.
Countersunk screw
Unit photodiode
Unit Peltier element
Heating unit Rubber strip
PCB RR-126
Attention: The rubber strip must be inserted in the gap.
RIELE BERLIN
Photometer 5010 V5+
5.4.4
04.05.2006
5.4
ASSEMBLING / DISMANTLING Unit photodiode
Unscrew the two screws.
PCB RR-126
Remove the unit photodiode and the plate. Photodiode
Plate
Attention: These parts should not be dismantled as an adjustment is necessary.
C6
RIELE BERLIN
Photometer 5010 V5+ B6
D6
5.4.5
04.05.2006
5.5
ASSEMBLING / DISMANTLING
Optical unit: Filter wheel, motor filter wheel, motor peristaltic pump, light barrier Optical unit
Remove Lamp shield.
Unscrew the two screws.
RIELE BERLIN
Photometer 5010 V5+
5.5.1
04.05.2006
5.5
ASSEMBLING / DISMANTLING The optical unit is divided in two parts. Motor peristaltic pump
Lamp holder
Motor filter wheel
Light barrier filter wheel
Unscrew the grub screw by allen key and remove filter wheel.
Unscrew the grub screw by allen key and remove peristaltic pump wheel.
Allen key
Unscrew the two crosshead screws and the two countersunk screws of motor peristaltic pump. Unscrew the two countersunk screws of optic block. Unscrew the four countersunk screws of motor filter wheel.
Optic block
Light barrier filter wheel
RIELE BERLIN
Photometer 5010 V5+
5.5.2
04.05.2006
5.5
ASSEMBLING / DISMANTLING In case of assembling: One of the holes situated on the border line of the filter wheel has to be positioned near to the hole situated on the mounting plate.
In case of assembling: Use two strips with a thickness of around 0.4 mm for adjusting the filter wheel. The strips have to be inserted as a checking and adjust gauge between filter wheel and mounting plate.
example for a strip
In case of assembling: Use two strips with a thickness of around 0.4 mm for adjusting the pump wheel. The strips have to be inserted as a checking and adjust gauge between pump wheel and mounting plate.
example for a strip
The light barrier must not scrape at the border line of the filter wheel.
Mounting plate
Border line
RIELE BERLIN
Photometer 5010 V5+
5.5.3
04.05.2006
5.6
ASSEMBLING / DISMANTLING
Power supply
Remove plug P1.
Remove plastic cover of the terminal.
Unscrew the three lens head screws labeled with L, N and PE.
Unscrew the cylinder head screw.
Unscrew the cylinder head screw.
Move the AC/DC power supply to the right.
RIELE BERLIN
Photometer 5010 V5+
5.6.1
04.05.2006
5.7
ASSEMBLING / DISMANTLING
Motherboard The following procedure describes the assembling and dismantling of the motherboard.
A7
Attention: The PCB includes an active battery supplying the RAM and the real time clock. To avoid a short circuit caused by e.g. a screw driver or a dropped screw. Pay attention to the metallic spacers between PCB and base plate in case of relocating. Unscrew the connection of the transistors T3/BD912 and T5/TIP132 (A7).
Unscrew the six cylinder head screws (B7).
B7
12 connections have to be unplugged. The labels of the plugs are: P1, P2, P3, P4; P5; P7, P8, P11, P13, P14, P20, P21 Attention: One must not swap the two plugs P5 and P13.
A7 The picture shows the base plate of the motherboard. Before mounting use heat conducting paste at location (C7). The transistors T3/BD912 and T5/TIP132 (C7) must be isolated from the base plate by a mica washer.
C7
RIELE BERLIN
Photometer 5010 V5+
5.7.1
04.05.2006
6.1
COMMUNICATION PROTOCOL
The settings of the serial interface are as follows: Type of interface: Baud rate: Data format: Parity: Protocol:
RS 232 C 9600 bit/s 7 data bits, 1 stop bit even STX - [string] - ETX – BCC ( STX = start of text, ETX = end of text, BCC = block code character ) The BCC is generated from the data string including the ETX (not STX). Parity of BCC is even.
Checksum:
Hardware connection:
1:1 data cable with the following pin configuration: 25-pin 9-pin Photometer 5010 V5+ 9-pin female 3 2 2 RXD (Receive Data) 2 3 3 TXD (Transmit Data) 7 5 5 GND (Signal Ground) 7 RTS (Request to send) 8 CTS (Clear to send)
The procedure of communication is as follows: First activate the remote mode of the Photometer 5010 V5+ starting serial interface utilities by [UTILITIES] -> [MENU SERIAL COM] -> [REMOTE MODE] (OM chapter 7.2.5.2). The communication starts with a block where the photometer (master) enables the computer (slave) to send a command (ACK = acknowledge, NAK = not acknowledge): Photometer 5010 V5+ Computer STX MØ ETX BCC =>
16 bit) or higher as the measurement, ADC overflow
repeat dark level adjustment; check lamp / filter; check blank
6
all multiplexer positions are too bright/too dark at setting to zero
repeat dark level adjustment; check filter / lamp; check blank
7
mathematical overflow, at measurement calculation
check filter; check standard; check measuring solution
8
check sum error in the data record of the dark offset
repeat dark level adjustment
9
check sum error in the data record of the device basic setting (status, ADC correction)
automatic error remedy
10
division by a too small value (< 0.001 A)
check filter; check standard; check measuring solution
11
invalid calibration curve
Select valid number
12
setting to zero not possible (zero value is < 32768 cycle) check lamp; check filter; check zero solution
13
setting to zero not possible (zero value is > 983039 cycle)
check lamp; check filter; check zero solution
14
invalid standard
measure valid standard solution
15
no parameter memory vacant (too little memory for nonlinear methods)
delete a no longer actual nonlinear method
16
method no. is occupied
select other method no.; delete a no longer actual nonlinear method
17
check sum error in the parameter memory (nonlinear method)
program method newly
18
at calculation overflows in nonlinear method
check factor; check parameter
19
clock malfunction
20
overflow at Kinetic
check measuring solution
21
overflow at Kinetic
check measuring solution
22
overflow at Kinetic
check measuring solution
23
overflow at Kinetic
check measuring solution
24
overflow at Kinetic
check measuring solution
25
overflow at Kinetic
check measuring solution
26
overflow at Kinetic
check measuring solution
RIELE BERLIN
Photometer 5010 V5+
7.1
10.04.2007
7
TROUBLE SHOOTING
27
overflow at Kinetic
check measuring solution
28
overflow at Kinetic
check measuring solution
29
wrong input of deltas or time per delta
restart method
30
battery empty
contact service partner
31
communication: wrong data format
contact service partner
32
communication: sent data not plausible / not interpretable
contact service partner
33
communication: mentioned module does not answer in a certain time
check interconnecting cable; check mentioned module
34
communication: overflow send/receive buffer
reduce amount of data at the communication partner
35
remote control: wrong method number
external software problem
36
remote control: unknown command
external software problem
37
remote control: wrong data format
external software problem
38
- not occupied -
39
- not occupied -
40
timeout at reception from module 2
switch off/on device
41
check sum error at reception from module 2
switch off/on device
42
NAK at reception from module 2
switch off/on device
43
- not occupied -
44
error at pump calibration - pump tube not clamped; - no liquid aspirated; - too much liquid aspirated during calibrating; - power of the pump too small; - motor does not rotate or stops; - connectors of tubes leaky; - bubble detector works falsely; - aspiration tube blocked; - aspiration tube badly stained
45
error at sipping solution - pump tube not clamped; - no liquid aspirated; - power of the pump too small; - motor does not rotate or stops; - connectors of tubes leaky; - bubble detector works falsely; - aspiration tube blocked; - aspiration tube badly stained
46
filter position out of tolerance
47
+ 12 VDC out of range
48
- not occupied -
49
- not occupied -
50
- not occupied -
51
- not occupied -
RIELE BERLIN
- clamp pump tube; - control volume of aspiration; - control connectors of tubes; - the plug of the bubble detector has a bad contact; - the cable of the bubble detector has a bad contact; - adjust bubble detector (OM chapter 7.2.4); - replace aspiration tube (OM chapter 8.7)
- clamp pump tube; - adjust bubble detector (OM chapter 7.2.4); - control volume of aspiration; - control connectors of tubes; - do a calibration of peristaltic pump (OM chapter 7.2.4); - the plug of the bubble detector has a bad contact; - the cable of the bubble detector has a bad contact; - replace aspiration tube (OM chapter 8.8)
contact service partner
Photometer 5010 V5+
7.2
10.04.2007
7
TROUBLE SHOOTING
52
timeout printer internal
the internal printer is temporarily disconnected
53
set of data points is missing
check multi-standard functions
54
nonlinear measurement: value out of range
check the absorbance of the sample
55
number of given data points < 2
add data points
56
checksum of operating system damaged
contact service partner
57
memory of patients data management is completely filled
delete patient data (OM chapter 7.2.8)
58
checksum of patients data damaged
delete patient data (OM chapter 7.2.8)
59
error in the automatic measuring operation (remote)
check interface
60
error at multiplexer of operational amplifier
contact service partner
61
error at multiplexer of bubble detector
contact service partner
62
free method number not found
check method memory
63
wrong address at multi-standard loading
check multi-standards
64
current method not found in monthly memory
check QC data of method
65
more than 50 QC methods defined
delete unused QC methods
66
internal clock is off. QC data not storable
switch on internal clock
67
BCC error in dataset of QC method values
check current method
68
free monthly memory not found at QC
delete unused QC methods
69
free space in daily memory not found at QC
empty QC daily memory
70
error at QC calculation
check QC data
71
QC serum not found
check QC data
No.
(possible) Causes
Remedy
the operating system behaves like a Photometer 4040
set the type of operating system to Photometer 5010 (chapter 6.4).
RIELE BERLIN
Photometer 5010 V5+
7.3
10.04.2007
8
SPARE PARTS
sorted by REF REF
DESCRIPTION
090-063
Thermistor
090-064
Secondary calibration standards, 4 pcs, certified
501-002
Data cable serial interface
5010-001
Tool kit filter exchange
5010-016
AC/DC power supply
5010-018
Fuses for line power, 10 pcs
5010-022
Fuses T3.15A / 250V, 10 pcs
5010-023
Fuses T6.3A / 250V, 10 pcs
5010-050
Pump tube with joints
5010-051
EPROM 5010 V5
5010-052
Printer mechanism 8NAD
5010-053
Printer controller 8NAD
5010-054
Bottom case
5010-055
Metal tube inlet
5010-056
Unit photodiode
5010-057
Touchscreen
5010-059
Lamp holder
5010-060
Step motor pump
5010-061
Step motor filter wheel
5010-062
Unit Peltier element
5010-063
Lightbarrier w. plug filterwheel
5010-064
Top case
5010-065
Aspiration tubing 185 mm, 2 pcs
5010-066
Outlet tube cuvette, 5 pcs
5010-067
Touch panel
5010-068
Unit cuvette adaptor
5010-069
Connector bubble detector
5010-070
Plastic foil touchscreen
5010-071
Top case pre-assembled
5010-072
Microswitch with plug
5010-073
Top cover small for printer
5010-120
PCB RR-120 Motherboard
5010-121
PCB RR-121 Motor driver
5010-122
PCB RR-122 Touchscreen
5010-670
Interference filter 670nm
5010-690
Interference filter 690nm
5010-700
Interference filter 700nm
0369616001
Interference filter 623nm
0369624001
Interference filter 436nm
RIELE BERLIN
Photometer 5010 V5+
8.1
18.05.2006
8
SPARE PARTS
sorted by REF REF
DESCRIPTION
0552402001
Mains cable
0554871001
Halogen lamp 12V/20W
0745235001
Printer ribbon, 5 pcs
1010735001
Printer ribbon, 1 pc
1268457001
Printer paper, 5 rolls
1704796001
Flow-through cuvette adaptor
1704800001
Standard cuvette adaptor
1704818001
Dust cover
1704834001
Waste tubing, 2 pcs
1706853001
Axis printer paper
1706870001
Bubble detector with plug
1706896001
Carton Photometer
1706926001
Cuvette lense kit
1706985001
Fan
1706993001
Interference filter 340nm
1707019001
Interference filter 405nm
1707027001
Interference filter 492nm
1707035001
Interference filter 546nm
1707043001
Interference filter 578nm
1707159001
Holding spring for disp. cuvette
1707469001
Rubber foot, 4 pcs
1707655001
Printer connection cable
1804553001
Step motor driver
RIELE BERLIN
Photometer 5010 V5+
8.2
18.05.2006
8
SPARE PARTS
sorted by DESCRIPTION REF
DESCRIPTION
5010-016
AC/DC power supply
5010-065
Aspiration tubing 185 mm, 2 pcs
1706853001 5010-054 1706870001 1706896001 5010-069 1706926001 501-002 1704818001 5010-051
Axis printer paper Bottom case Bubble detector with plug Carton Photometer Connector bubble detector Cuvette lense kit Data cable serial interface Dust cover EPROM 5010 V5
1706985001
Fan
1704796001
Flow-through cuvette adaptor
5010-018
Fuses for line power, 10 pcs
5010-022
Fuses T3.15A / 250V, 10 pcs
5010-023
Fuses T6.3A / 250V, 10 pcs
0554871001
Halogen lamp 12V/20W
1707159001
Holding spring for disp. cuvette
1706993001
Interference filter 340nm
1707019001
Interference filter 405nm
0369624001
Interference filter 436nm
1707027001
Interference filter 492nm
1707035001
Interference filter 546nm
1707043001
Interference filter 578nm
0369616001
Interference filter 623nm
5010-670
Interference filter 670nm
5010-690
Interference filter 690nm
5010-700
Interference filter 700nm
5010-059
Lamp holder
5010-063 0552402001
Lightbarrier w. plug filterwheel Mains cable
5010-055
Metal tube inlet
5010-072
Microswitch with plug
5010-066
Outlet tube cuvette, 5 pcs
5010-120
PCB RR-120 Motherboard
5010-121
PCB RR-121 Motor driver
5010-122
PCB RR-122 Touchscreen
5010-070
Plastic foil touchscreen
1707655001
Printer connection cable
RIELE BERLIN
Photometer 5010 V5+
8.3
18.05.2006
8
SPARE PARTS
sorted by DESCRIPTION REF DESCRIPTION 5010-053
Printer controller 8NAD
5010-052
Printer mechanism 8NAD
1268457001
Printer paper, 5 rolls
1010735001
Printer ribbon, 1 pc
0745235001
Printer ribbon, 5 pcs
5010-050
Pump tube with joints
1707469001 090-064
Rubber foot, 4 pcs Secondary calibration standards, 4 pcs, certified
1704800001
Standard cuvette adaptor
1804553001
Step motor driver
5010-061
Step motor filter wheel
5010-060
Step motor pump
090-063
Thermistor
5010-001
Tool kit filter exchange
5010-064
Top case
5010-071
Top case pre-assembled
5010-073
Top cover small for printer
5010-067
Touch panel
5010-057
Touchscreen
5010-068
Unit cuvette adaptor
5010-062
Unit Peltier element
5010-056
Unit photodiode
1704834001
RIELE BERLIN
Waste tubing, 2 pcs
Photometer 5010 V5+
8.4
18.05.2006
9.1
CALIBRATION PROCEDURE OF PHOTOMETER 5010 SUBJECT: CUVETTE TEMPERATURE
1. Purpose This calibration procedure describes the calibration of the cuvette temperature of Photometer 5010. The meaning of calibration is to determine the difference between the true temperature and the set temperature. The procedure is to carry out for the temperatures which are used by the customer.
2. General description and measuring theory The temperature is measured with a selected thermistor-probe. The thermistor is supplied by RIELE. The certified tolerance of this thermistor is ± 0.1 °C. The photometer-cuvette temperature is measured in case of using the single cuvette adaptor in an aluminium-dummy cuvette or in case of using the flow-through cuvette adaptor in the original flow-through cuvette. The resistance of the thermistor is measured by a 4½ digit digital multimeter (DMM). The requirements of the DMM are given in chapter 7. The corresponding temperature is listed in chapter 8. The implementation of the whole procedure lasts approximately 1.5 hours.
3. Operating conditions The calibration should be performed under normal operating conditions, i.e. • • •
Room temperature: 15 °C to 30 °C Relative humidity: 40 % to 80 % No ventilation or draft
Note that the variation of the room temperature should not exceed ± 2 °C during the calibration process.
4. Traceability of the calibration standards The traceability to the National Temperature Standard is given by selected thermistor and a calibrated DMM. The last Calibration Certificate of both, the DMM and the thermistor, must not be older than 12 months. If the 24 month accuracy of the DMM fulfils the requirements in chapter 7, the last DMM Calibration Certificate can be up to 24 months old.
5. Test procedure a. In case of using the single cuvette adaptor insert the dummy-cuvette instead of a square cell. In case of using the flow-through adaptor remove the joint inlet tube cuvette / aspirate tubing and the outlet tube cuvette. Pipette 200 µl distilled water in the dummy-cuvette or the flow-through cuvette. b.
Connect the thermistor lead to the two-wire resistance input of the DMM.
c.
Insert the thermistor-probe into the hole of the photometer dummy-cuvette or the hole of the outlet tube cuvette of the flow-through cuvette. Caution: Handle the delicate probe with care! Pay attention to the correct position of the probe. Avoid bubbles within the aperture of the flow-through cuvette.
d.
Switch on the DMM and Photometer 5010 and wait 15 minutes for warming up.
e.
Select the two-wire resistance function and appropriate range on the DMM. The resistance of the thermistor is in the range of 6 kOhm up to 10 kOhm for the temperature range of 25 °C up to 37 °C. The table of resistance of thermistor (REF 090-063) shows the assigned resistance for a given temperature (see chapter 9 – item 8).
f.
Enter the service program level.
RIELE BERLIN
Photometer 5010 V5+
9.1.1
15.05.2006
9.1
CALIBRATION PROCEDURE OF PHOTOMETER 5010 SUBJECT: CUVETTE TEMPERATURE For additional information see chapter 3.3 – SERVICE TOOLS.
g.
Perform a system initialization by applying service program item 1 – S1 - System initialization.
h.
Enter the service program item 9 – S9 Temperature ON/OFF.
i.
Select the temperature 25° C.
j.
Check the temperature control by using service program item 9 – S9 Temperature ON/OFF. As long as temperature is not stabilized, the stability is showing a value different of zero. It needs minimum five minutes until a stabilized temperature level is reached. Wait 10 minutes furthermore.
k.
Read the displayed measurement of DMM in Ohm. Look up in the table of resistance the appropriate set of values. Calculate by performing a linear interpolation the assigned temperature value (see chapter 9 – item 8).
l.
Make an automatic temperature calibration by using service program item 12 – S12 Temperature calibration.
m.
Check the temperature control by using service program item 9 – S9 Temperature ON/OFF. As long as temperature is not stabilized, the stability is showing a value different of zero. It needs minimum five minutes until a stabilized temperature level is reached. Wait 5 minutes furthermore.
n.
Read the displayed measurement of DMM in Ohm. Look up in the table of resistance the appropriate set of values. The temperature value should show the desired value.
o.
Read the required value and subtract the value 3000. The result is called temperature value. Note this value in calibration protocol combined with adaptor type and temperature level. See for additional information in chapter 4.40 – MOTHER BOARD – Thermal adjustment.
p.
Repeat above mentioned steps with the temperature levels 25 °C, 30 °C and 37 °C for both types of adaptors.
6. Documentation For documentation use a form containing following table: 25 °C 30 °C 37 °C
Single cuvette adaptor Temperature value Temperature value Temperature value
Flow-through adaptor Temperature value Temperature value Temperature value
7. Requirements for the Digital Multimeter (DMM) The DMM for the resistance-measurements has to fulfil the following requirements: • • •
12 month accuracy of resistance measurements within the 0 – 30 KOhm range: Current for resistance measurement: Latest Calibration Certificate:
0.05 % + 2 digits ≤ 15 µA not older than 12 months
If the 24 months resistance-accuracy fulfils the above accuracy requirements, the latest Calibration Certificate can be ≤ 24 months old.
RIELE BERLIN
Photometer 5010 V5+
9.1.2
15.05.2006
9.1
CALIBRATION PROCEDURE OF PHOTOMETER 5010 SUBJECT: CUVETTE TEMPERATURE
8. Table of resistance – Thermistor REF 090-063 Temperature
Resistance
Temperature
Resistance
Temperature
Resistance
[° C]
[Ohm]
[° C]
[Ohm]
[° C]
[Ohm]
23,5 23,6 23,7 23,8 23,9 24,0 24,1 24,2 24,3 24,4 24,5 24,6 24,7 24,8 24,9 25,0 25,1 25,2 25,3 25,4 25,5 25,6 25,7 25,8 25,9 26,0 26,1 26,2 26,3 26,4 26,5
10682 10636 10589 10543 10496 10450 10404 10358 10313 10268 10223 10178 10133 10089 10044 10000 9956 9913 9870 9827 9784 9742 9700 9657 9615 9573 9531 9490 9449 9408 9368
28,5 28,6 28,7 28,8 28,9 29,0 29,1 29,2 29,3 29,4 29,5 29,6 29,7 29,8 29,9 30,0 30,1 30,2 30,3 30,4 30,5 30,6 30,7 30,8 30,9 31,0 31,1 31,2 31,3 31,4 31,5
8590 8553 8517 8480 8443 8407 8371 8335 8300 8264 8230 8195 8161 8126 8092 8057 8023 7989 7955 7922 7888 7855 7822 7789 7756 7723 7690 7658 7626 7593 7562
35,5 35,6 35,7 35,8 35,9 36,0 36,1 36,2 36,3 36,4 36,5 36,6 36,7 36,8 36,9 37,0 37,1 37,2 37,3 37,4 37,5 37,6 37,7 37,8 37,9 38,0 38,1 38,2 38,3 38,4 38,5
6397 6371 6345 6319 6293 6267 6241 6216 6191 6166 6141 6116 6091 6066 6042 6017 5993 5968 5944 5920 5896 5872 5848 5824 5801 5777 5754 5730 5710 5684 5661
RIELE BERLIN
Photometer 5010 V5+
9.1.3
15.05.2006
9.1
CALIBRATION PROCEDURE OF PHOTOMETER 5010 SUBJECT: CUVETTE TEMPERATURE
9. Linear interpolation This procedure describes the performing of a linear interpolation in case of a given resistance R. The assigned temperature is called T. If the resistance R is found in the table, get the appropriate temperature value and finish this chapter. If the actual temperature of the thermistor is in the range of the required temperature ± 1.5 °C, two values of resistance can be read from table with the relation:
R0
>
R
>
R1
The resistances R0 and R1 form an interval. They are a set of data points next to the resistance R. The assigned temperature values of the resistances R0
and R1 are called T0 and T1.
The assigned temperature T can be calculated by the formula:
T
=
T0
+
(T1 – T0) × (R - R0) / (R1 – R0)
Example: In case of a given resistance R with the value 9978 Ohm you can find the interpolated temperature T by:
RIELE BERLIN
T
=
25 °C
+
T
=
25.05 °C
0.1 °C × (9978 - 10000) / (9956 – 10000)
Photometer 5010 V5+
9.1.4
15.05.2006
