Dewi Kartika Sari - Worksheet 4 - P2 [PDF]

Citation preview

WORKSHEET 4 Penggalian Database Biologis di Internet (Bioinformatika) Judul Praktikum



: Penggalian Database Biologis di Internet (Bioinformatika)



Tanggal



: Selasa, 10 Mei 2001



Nama NIM



: Dewi Kartika Sari : 1808010107



Bagian A 1. Tampilkan hasil alignment sekuen asam amino estrogen receptor dari enam spesies tersebut. Jawab : >sp|P03372|ESR1_HUMAN Estrogen receptor OS=Homo sapiens OX=9606 GN=ESR1 PE=1 SV=2 MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAY EFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPF LQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAK ETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQAC RLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSAGDMRAANLWPSPLMIKR SKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLADRELVHMINW AKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEG MVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLD KITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLL LEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV >sp|P49884|ESR1_BOVIN Estrogen receptor OS=Bos taurus OX=9913 GN=ESR1 PE=1 SV=3 MTMTLHTKASGMALLHQIQANELEPLNRPQLKIPLERPLGEVYMDSSKPAVYNYPEGAAY DFNAAAPASAPVYGQSGLPYGPGSEAAAFGANGLGAFPPLNSVSPSPLVLLHPPPQPLSP FLHPHGQQVPYYLENESSGYAVREAGPPAYYRPNSDNRRQGGRERLASTSDKGSMAMESA KETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQA CRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRNEAVPSGDMRAANLWPSPIMIK HTKKNSPVLSLTADQMISALLEAEPPIIYSEYDPTRPFSEASMMGLLTNLADRELVHMIN WAKRVPGFVDLALHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVE GMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLRSLEEKDHIHRVL DKITDTLIHLMAKAGLTLQQQHRRLAQLLLILSHFRHMSNKGMEHLYSMKCKNVVPLYDL LLEMLDAHRLHAPANFGSAPPEDVNQSQLAPTGCTSSHSLQTYYITGEAENFPSTV >sp|Q29040|ESR1_PIG Estrogen receptor OS=Sus scrofa OX=9823 GN=ESR1 PE=1 SV=1 MTMTLHTKASGMALLHQIQANELEPLNRPQLKIPLERPLGEVYVDSSKPAVYNYPEGAAY DFNAAAAASAPVYGQSGLAYGPGSEAAAFGANGLGGFQPLNSVSPSPLVLLHPPPQLSPF LHPHGQQVPYYLENEPSGYAVREAGPPAFYRPNSDNRRQGGRERLASTSDKGSMAMESAK



1



ETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQAC RLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRNEAVPPGDMRSANLWPSPLLIKH TKKNSPVLSLTADQMISALLEAEPPIIYSEYDPTRPLSEASMMGLLTNLADRELVHMINW AKRVPGFLDLSLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEG MVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLD KITDTLIHLMAKAGLTLQQQHRRLAQLLLILSHFRHMSNKGMEHLYNMKCKNVVPLYDLL LEMLDAHRLHAPTNLGGPPPEDMSQSQLATSGSTPSHSLQMYYITGEAENFPTTI



>sp|P57717|ESR1_DANRE Estrogen receptor OS=Danio rerio OX=7955 GN=esr1 PE=2 SV=2 MYPKEEHSAGGISSSVNYLDGAYEYPNPTQTFGTSSPAEPASVGYYPAPPDPHEEHLQTL GGGSSSPLMFAPSSPQLSPYLSHHGGHHTTPHQVSYYLDSSSSTVYRSSVVSSQQAAVGL CEELCSATDRQELYTGSRAAGGFDSGKETRFCAVCSDYASGYHYGVWSCEGCKAFFKRSI QGHNDYVCPATNQCTIDRNRRKSCQACRLRKCYEVGMMKGGIRKDRGGRSVRRERRRSSN EDRDKSSSDQCSRAGVRTTGPQDKRKKRSGGVVSTLCMSPDQVLLLLLGAEPPAVCSRQK HSRPYTEITMMSLLTNMADKELVHMIAWAKKVPGFQDLSLHDQVQLLESSWLEVLMIGLI WRSIHSPGKLIFAQDLILDRSEGECVEGMAEIFDMLLATVARFRSLKLKLEEFVCLKAII LINSGAFSFCSSPVEPLMDNFMVQCMLDNITDALIYCISKSGASLQLQSRRQAQLLLLLS HIRHMSNKGMEHLYRMKCKNRVPLYDLLLEMLDAQRFQSSGKVQRVWSQSEKNPPSTPTT SSSSSNNSPRGGAAAIQSNGACHSHSPDP



>sp|Q9TV98|ESR1_HORSE Estrogen receptor OS=Equus caballus OX=9796 GN=ESR1 PE=2 SV=1 MTMTLHTKASGMALLHQIQGNELETLNLPQFKIPLERPLGEVYVESSKPPVYDYPEGAAY DFNAAAAASASVYGQSGLAYGPGSEAAAFGANGLGGFPPLNSVSPSQLMLLHPPPQLSPY LHPPGQQVPYYLENEPSGYSVCEAGPQAFYRPNADNRRQGGRERLASSGDKGSMAMESAK ETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQAC RLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRNEAGPSGDRRPANFWPSPLLIKH TKKISPVLSLTAEQMISALLDAEPPVLYSEYDATRPFNEASMMGLLTNLADRELVHMINW AKRVPGFVDLSLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEG MVEIFDMLLATSSRLRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLD KMTDTLIHLMAKAGLTLQQHRRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLL EMLDAHRLHAPANHGGAPMEETNQSQLATTGSTSPHSMQTYYITGEAEGFPNTI >sp|P16058|ESR1_ONCMY Estrogen receptor OS=Oncorhynchus mykiss OX=8022 GN=esr1 PE=2 SV=3 MLVRQSHTQISKPLGAPLRSRTTLESHVISPPKLSPQQPTTPNSNMYPEETRGGGGAAAF NYLDGGYDYTAPAQGPAPLYYSTTPQDAHGPPSDGSMQSLGSSPTGPLVFVSSSPQLSPQ LSPFLHPPSHHGLPSQSYYLETSSTPLYRSSVVTNQLSASEEKLCIASDRQQSYSAAGSG VRVFEMANETRYCAVCSDFASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTMDRN RRKSCQACRLRKCYEVGMVKGGLRKDRGGRVLRKDKRYCGPAGDREKPYGDLEHRTAPPQ DGGRNSSSSLNGGGGWRGPRITMPPEQVLFLLQGAEPPALCSRQKVARPYTEVTMMTLLT SMADKELVHMIAWAKKVPGFQELSLHDQVQLLESSWLEVLMIGLIWRSIHCPGKLIFAQD LILDRSEGDCVEGMAEIFDMLLATVSRFRMLKLKPEEFVCLKAIILLNSGAFSFCSNSVE SLHNSSAVESMLDNITDALIHHISHSGASVQQQPRRQAQLLLLLSHIRHMSNKGMEHLYS IKCKNKVPLYDLLLEMLDGHRLQSPGKVAQAGEQTEGPSTTTTTSTGSSIGPMRGSQDTH IRSPGSGVLQYGSPSSDQMPIP



2. Isikan tabel Persentase identitas alignment asam amino untuk reseptor estrogen berikut berdasarakan hasil sequence alignment yang ada lakukan. 2



Jawab : Spesies



Manusia



Sapi



Babi



Kuda



Zebrafish



Ikan rainbow trout



Manusia



100



91,93



91,93



90,24



51,50



49,83



Sapi



91,93



100



95,13



90,91



51,22



50,61



Babi



91,93



95,13



100



91,41



51,13



50,70



Kuda



90,24



90,91



91,41



100



50,66



50,44



Zebrafish



51,50



51,22



51,13



50,66



100



66,61



Ikan rainbow trout



49,83



50,61



50,70



50,44



66,61



100



3. Tampilkan pohon dendogram yang menunjukan hubungan filogenetik dari estrogen receptor keenam spesies tersebut. Jawab :



Bagian B. 1. Tampilkan screen shoot layar hasil pencarian anda mengenai protein OSR1. Jawab :



3



4